Experiment: LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12695 and BWI76_RS12700 are separated by 277 nucleotides BWI76_RS12700 and BWI76_RS12705 overlap by 50 nucleotides BWI76_RS12705 and BWI76_RS12710 are separated by 43 nucleotides
BWI76_RS12695: BWI76_RS12695 - zinc transporter ZntB, at 2,646,758 to 2,647,741
_RS12695
BWI76_RS12700: BWI76_RS12700 - hypothetical protein, at 2,648,019 to 2,648,273
_RS12700
BWI76_RS12705: BWI76_RS12705 - ATP-dependent RNA helicase, at 2,648,224 to 2,649,597
_RS12705
BWI76_RS12710: BWI76_RS12710 - tRNA 2-thiocytidine(32) synthetase TtcA, at 2,649,641 to 2,650,576
_RS12710
Position (kb)
2648
2649
2650 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2647.398 kb on + strand, within BWI76_RS12695 at 2647.475 kb on - strand, within BWI76_RS12695 at 2647.581 kb on + strand, within BWI76_RS12695 at 2647.706 kb on + strand at 2647.840 kb on + strand at 2647.864 kb on + strand at 2647.872 kb on - strand at 2648.068 kb on + strand, within BWI76_RS12700 at 2648.076 kb on - strand, within BWI76_RS12700 at 2648.396 kb on + strand, within BWI76_RS12705 at 2648.424 kb on - strand, within BWI76_RS12705 at 2648.438 kb on - strand, within BWI76_RS12705 at 2648.438 kb on - strand, within BWI76_RS12705 at 2648.662 kb on + strand, within BWI76_RS12705 at 2648.673 kb on + strand, within BWI76_RS12705 at 2648.815 kb on + strand, within BWI76_RS12705 at 2649.044 kb on + strand, within BWI76_RS12705 at 2649.044 kb on + strand, within BWI76_RS12705 at 2649.533 kb on + strand at 2649.618 kb on - strand at 2649.618 kb on - strand at 2649.766 kb on + strand, within BWI76_RS12710 at 2649.825 kb on - strand, within BWI76_RS12710 at 2649.843 kb on + strand, within BWI76_RS12710 at 2649.878 kb on - strand, within BWI76_RS12710 at 2649.944 kb on - strand, within BWI76_RS12710 at 2650.099 kb on + strand, within BWI76_RS12710 at 2650.159 kb on + strand, within BWI76_RS12710 at 2650.198 kb on - strand, within BWI76_RS12710 at 2650.426 kb on + strand, within BWI76_RS12710
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL remove 2,647,398 + BWI76_RS12695 0.65 +0.2 2,647,475 - BWI76_RS12695 0.73 -1.6 2,647,581 + BWI76_RS12695 0.84 +0.5 2,647,706 + +1.2 2,647,840 + +0.2 2,647,864 + +0.6 2,647,872 - +0.3 2,648,068 + BWI76_RS12700 0.19 -0.8 2,648,076 - BWI76_RS12700 0.22 +0.1 2,648,396 + BWI76_RS12705 0.13 +0.7 2,648,424 - BWI76_RS12705 0.15 +0.5 2,648,438 - BWI76_RS12705 0.16 +0.3 2,648,438 - BWI76_RS12705 0.16 +1.8 2,648,662 + BWI76_RS12705 0.32 -2.0 2,648,673 + BWI76_RS12705 0.33 +1.7 2,648,815 + BWI76_RS12705 0.43 +0.8 2,649,044 + BWI76_RS12705 0.60 +0.1 2,649,044 + BWI76_RS12705 0.60 +0.5 2,649,533 + +1.0 2,649,618 - +0.2 2,649,618 - -2.5 2,649,766 + BWI76_RS12710 0.13 -1.7 2,649,825 - BWI76_RS12710 0.20 +0.4 2,649,843 + BWI76_RS12710 0.22 -0.6 2,649,878 - BWI76_RS12710 0.25 +0.4 2,649,944 - BWI76_RS12710 0.32 +0.1 2,650,099 + BWI76_RS12710 0.49 +0.9 2,650,159 + BWI76_RS12710 0.55 -1.2 2,650,198 - BWI76_RS12710 0.60 -1.0 2,650,426 + BWI76_RS12710 0.84 -1.8
Or see this region's nucleotide sequence