Experiment: LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS11410 and BWI76_RS11415 are separated by 128 nucleotides BWI76_RS11415 and BWI76_RS11420 are separated by 314 nucleotides BWI76_RS11420 and BWI76_RS11425 are separated by 35 nucleotides BWI76_RS11425 and BWI76_RS11430 are separated by 297 nucleotides BWI76_RS11430 and BWI76_RS11435 overlap by 6 nucleotides BWI76_RS11435 and BWI76_RS11440 overlap by 14 nucleotides
BWI76_RS11410: BWI76_RS11410 - leucine efflux protein, at 2,404,826 to 2,405,464
_RS11410
BWI76_RS11415: BWI76_RS11415 - hypothetical protein, at 2,405,593 to 2,405,856
_RS11415
BWI76_RS11420: BWI76_RS11420 - hypothetical protein, at 2,406,171 to 2,406,368
_RS11420
BWI76_RS11425: BWI76_RS11425 - GAF domain/diguanylate cyclase, at 2,406,404 to 2,407,417
_RS11425
BWI76_RS11430: BWI76_RS11430 - lipoprotein, at 2,407,715 to 2,407,954
_RS11430
BWI76_RS11435: BWI76_RS11435 - hypothetical protein, at 2,407,949 to 2,408,293
_RS11435
BWI76_RS11440: BWI76_RS11440 - hypothetical protein, at 2,408,280 to 2,408,789
_RS11440
Position (kb)
2406
2407
2408 Strain fitness (log2 ratio)
-2
-1
0
1 at 2405.461 kb on - strand at 2405.737 kb on - strand at 2405.958 kb on - strand at 2405.973 kb on - strand at 2406.528 kb on - strand, within BWI76_RS11425 at 2406.891 kb on + strand, within BWI76_RS11425 at 2406.899 kb on - strand, within BWI76_RS11425 at 2406.899 kb on - strand, within BWI76_RS11425 at 2406.899 kb on - strand, within BWI76_RS11425 at 2407.016 kb on - strand, within BWI76_RS11425 at 2407.252 kb on - strand, within BWI76_RS11425 at 2407.602 kb on + strand at 2407.722 kb on + strand at 2407.750 kb on + strand, within BWI76_RS11430 at 2408.018 kb on - strand, within BWI76_RS11435 at 2408.109 kb on - strand, within BWI76_RS11435 at 2408.257 kb on + strand, within BWI76_RS11435
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL remove 2,405,461 - +0.1 2,405,737 - +1.1 2,405,958 - -0.1 2,405,973 - +0.6 2,406,528 - BWI76_RS11425 0.12 +0.0 2,406,891 + BWI76_RS11425 0.48 -0.6 2,406,899 - BWI76_RS11425 0.49 -0.7 2,406,899 - BWI76_RS11425 0.49 -0.2 2,406,899 - BWI76_RS11425 0.49 -1.3 2,407,016 - BWI76_RS11425 0.60 -1.9 2,407,252 - BWI76_RS11425 0.84 +0.0 2,407,602 + -0.1 2,407,722 + +0.7 2,407,750 + BWI76_RS11430 0.15 -0.9 2,408,018 - BWI76_RS11435 0.20 -0.9 2,408,109 - BWI76_RS11435 0.46 -1.5 2,408,257 + BWI76_RS11435 0.89 +1.7
Or see this region's nucleotide sequence