Experiment: LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10530 and BWI76_RS10535 overlap by 1 nucleotides BWI76_RS10535 and BWI76_RS10540 are separated by 16 nucleotides BWI76_RS10540 and BWI76_RS10545 are separated by 227 nucleotides BWI76_RS10545 and BWI76_RS10550 overlap by 1 nucleotides
BWI76_RS10530: BWI76_RS10530 - GntR family transcriptional regulator with, at 2,225,453 to 2,226,169
_RS10530
BWI76_RS10535: BWI76_RS10535 - Asp/Glu/hydantoin racemase, at 2,226,169 to 2,226,912
_RS10535
BWI76_RS10540: BWI76_RS10540 - allantoinase, at 2,226,929 to 2,227,861
_RS10540
BWI76_RS10545: BWI76_RS10545 - hypothetical protein, at 2,228,089 to 2,228,472
_RS10545
BWI76_RS10550: BWI76_RS10550 - AtzE family amidohydrolase, at 2,228,472 to 2,229,869
_RS10550
Position (kb)
2226
2227
2228 Strain fitness (log2 ratio)
-2
-1
0
1 at 2225.939 kb on + strand, within BWI76_RS10530 at 2226.330 kb on - strand, within BWI76_RS10535 at 2226.357 kb on + strand, within BWI76_RS10535 at 2226.595 kb on - strand, within BWI76_RS10535 at 2226.975 kb on + strand at 2227.349 kb on + strand, within BWI76_RS10540 at 2227.392 kb on - strand, within BWI76_RS10540 at 2227.618 kb on - strand, within BWI76_RS10540 at 2227.644 kb on - strand, within BWI76_RS10540 at 2227.667 kb on - strand, within BWI76_RS10540 at 2227.754 kb on - strand, within BWI76_RS10540 at 2227.888 kb on + strand at 2227.909 kb on - strand at 2228.048 kb on + strand at 2228.077 kb on - strand at 2228.117 kb on - strand at 2228.716 kb on - strand, within BWI76_RS10550
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL remove 2,225,939 + BWI76_RS10530 0.68 +0.6 2,226,330 - BWI76_RS10535 0.22 -2.7 2,226,357 + BWI76_RS10535 0.25 -0.1 2,226,595 - BWI76_RS10535 0.57 +0.0 2,226,975 + -1.1 2,227,349 + BWI76_RS10540 0.45 +0.9 2,227,392 - BWI76_RS10540 0.50 +1.0 2,227,618 - BWI76_RS10540 0.74 +0.3 2,227,644 - BWI76_RS10540 0.77 +1.1 2,227,667 - BWI76_RS10540 0.79 -0.6 2,227,754 - BWI76_RS10540 0.88 +0.7 2,227,888 + -0.0 2,227,909 - +0.4 2,228,048 + +0.3 2,228,077 - +0.7 2,228,117 - +0.6 2,228,716 - BWI76_RS10550 0.17 +0.2
Or see this region's nucleotide sequence