Experiment: LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10365 and BWI76_RS10370 are separated by 15 nucleotides BWI76_RS10370 and BWI76_RS10375 are separated by 16 nucleotides BWI76_RS10375 and BWI76_RS10380 are separated by 13 nucleotides BWI76_RS10380 and BWI76_RS10385 are separated by 142 nucleotides
BWI76_RS10365: BWI76_RS10365 - class IV aminotransferase, at 2,195,080 to 2,196,045
_RS10365
BWI76_RS10370: BWI76_RS10370 - ABC transporter-like protein, at 2,196,061 to 2,196,840
_RS10370
BWI76_RS10375: BWI76_RS10375 - polar amino acid ABC transporter inner membrane subunit, at 2,196,857 to 2,197,522
_RS10375
BWI76_RS10380: BWI76_RS10380 - polar amino acid ABC transporter inner membrane subunit, at 2,197,536 to 2,198,207
_RS10380
BWI76_RS10385: BWI76_RS10385 - MmgE/PrpD family protein, at 2,198,350 to 2,199,759
_RS10385
Position (kb)
2196
2197
2198 Strain fitness (log2 ratio)
-1
0
1 at 2196.102 kb on - strand at 2196.210 kb on + strand, within BWI76_RS10370 at 2196.293 kb on - strand, within BWI76_RS10370 at 2196.369 kb on + strand, within BWI76_RS10370 at 2196.452 kb on - strand, within BWI76_RS10370 at 2196.630 kb on + strand, within BWI76_RS10370 at 2196.630 kb on + strand, within BWI76_RS10370 at 2196.630 kb on + strand, within BWI76_RS10370 at 2196.638 kb on - strand, within BWI76_RS10370 at 2196.638 kb on - strand, within BWI76_RS10370 at 2196.638 kb on - strand, within BWI76_RS10370 at 2196.844 kb on - strand at 2196.909 kb on + strand at 2196.924 kb on + strand, within BWI76_RS10375 at 2197.144 kb on - strand, within BWI76_RS10375 at 2197.233 kb on - strand, within BWI76_RS10375 at 2197.334 kb on - strand, within BWI76_RS10375 at 2197.551 kb on - strand at 2197.617 kb on - strand, within BWI76_RS10380 at 2197.683 kb on - strand, within BWI76_RS10380 at 2198.137 kb on + strand, within BWI76_RS10380 at 2198.140 kb on + strand at 2198.209 kb on + strand at 2198.262 kb on + strand at 2198.433 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^5 spores/mL remove 2,196,102 - +0.4 2,196,210 + BWI76_RS10370 0.19 +0.9 2,196,293 - BWI76_RS10370 0.30 -0.1 2,196,369 + BWI76_RS10370 0.39 -0.5 2,196,452 - BWI76_RS10370 0.50 -0.2 2,196,630 + BWI76_RS10370 0.73 +0.9 2,196,630 + BWI76_RS10370 0.73 -0.4 2,196,630 + BWI76_RS10370 0.73 -0.2 2,196,638 - BWI76_RS10370 0.74 +0.0 2,196,638 - BWI76_RS10370 0.74 +0.8 2,196,638 - BWI76_RS10370 0.74 +0.8 2,196,844 - -0.1 2,196,909 + +0.1 2,196,924 + BWI76_RS10375 0.10 +0.4 2,197,144 - BWI76_RS10375 0.43 +1.1 2,197,233 - BWI76_RS10375 0.56 +0.4 2,197,334 - BWI76_RS10375 0.72 +0.7 2,197,551 - +0.6 2,197,617 - BWI76_RS10380 0.12 +1.0 2,197,683 - BWI76_RS10380 0.22 +0.2 2,198,137 + BWI76_RS10380 0.89 +0.1 2,198,140 + +1.6 2,198,209 + +1.1 2,198,262 + -0.4 2,198,433 + -0.1
Or see this region's nucleotide sequence