Experiment: LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS27210 and BWI76_RS27215 overlap by 1 nucleotides BWI76_RS27215 and BWI76_RS27220 are separated by 59 nucleotides BWI76_RS27220 and BWI76_RS27225 are separated by 111 nucleotides BWI76_RS27225 and BWI76_RS27230 are separated by 245 nucleotides
BWI76_RS27210: BWI76_RS27210 - glycerol-3-phosphate dehydrogenase, at 5,648,179 to 5,649,195
_RS27210
BWI76_RS27215: BWI76_RS27215 - protein-export protein SecB, at 5,649,195 to 5,649,662
_RS27215
BWI76_RS27220: BWI76_RS27220 - glutaredoxin 3, at 5,649,722 to 5,649,973
_RS27220
BWI76_RS27225: BWI76_RS27225 - rhodanese-like domain-containing protein, at 5,650,085 to 5,650,516
_RS27225
BWI76_RS27230: BWI76_RS27230 - 2,3-bisphosphoglycerate-independent phosphoglycerate mutase, at 5,650,762 to 5,652,306
_RS27230
Position (kb)
5650
5651 Strain fitness (log2 ratio)
-2
-1
0
1 at 5649.738 kb on - strand at 5649.898 kb on - strand, within BWI76_RS27220 at 5649.958 kb on - strand at 5650.117 kb on - strand at 5650.164 kb on - strand, within BWI76_RS27225 at 5650.175 kb on - strand, within BWI76_RS27225 at 5650.472 kb on + strand, within BWI76_RS27225 at 5650.561 kb on + strand at 5650.569 kb on - strand at 5650.686 kb on - strand at 5650.861 kb on + strand at 5650.957 kb on - strand, within BWI76_RS27230 at 5650.957 kb on - strand, within BWI76_RS27230 at 5650.965 kb on + strand, within BWI76_RS27230 at 5651.007 kb on - strand, within BWI76_RS27230 at 5651.048 kb on + strand, within BWI76_RS27230 at 5651.056 kb on - strand, within BWI76_RS27230 at 5651.061 kb on - strand, within BWI76_RS27230 at 5651.118 kb on - strand, within BWI76_RS27230 at 5651.289 kb on + strand, within BWI76_RS27230 at 5651.290 kb on + strand, within BWI76_RS27230 at 5651.362 kb on + strand, within BWI76_RS27230 at 5651.400 kb on + strand, within BWI76_RS27230 at 5651.502 kb on - strand, within BWI76_RS27230
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL remove 5,649,738 - -0.5 5,649,898 - BWI76_RS27220 0.70 -0.6 5,649,958 - -0.3 5,650,117 - -0.9 5,650,164 - BWI76_RS27225 0.18 -1.5 5,650,175 - BWI76_RS27225 0.21 -0.5 5,650,472 + BWI76_RS27225 0.90 -2.6 5,650,561 + +0.0 5,650,569 - +0.7 5,650,686 - -1.3 5,650,861 + -1.0 5,650,957 - BWI76_RS27230 0.13 -0.6 5,650,957 - BWI76_RS27230 0.13 -0.3 5,650,965 + BWI76_RS27230 0.13 +1.0 5,651,007 - BWI76_RS27230 0.16 -1.1 5,651,048 + BWI76_RS27230 0.19 -0.7 5,651,056 - BWI76_RS27230 0.19 +0.3 5,651,061 - BWI76_RS27230 0.19 +1.0 5,651,118 - BWI76_RS27230 0.23 +0.3 5,651,289 + BWI76_RS27230 0.34 -1.0 5,651,290 + BWI76_RS27230 0.34 -0.1 5,651,362 + BWI76_RS27230 0.39 -1.6 5,651,400 + BWI76_RS27230 0.41 -0.5 5,651,502 - BWI76_RS27230 0.48 -0.7
Or see this region's nucleotide sequence