Experiment: LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS23950 and BWI76_RS23955 overlap by 4 nucleotides BWI76_RS23955 and BWI76_RS23960 are separated by 121 nucleotides BWI76_RS23960 and BWI76_RS23965 are separated by 90 nucleotides
BWI76_RS23950: BWI76_RS23950 - succinate CoA transferase, at 4,998,900 to 5,000,366
_RS23950
BWI76_RS23955: BWI76_RS23955 - LysR family transcriptional regulator, at 5,000,363 to 5,001,259
_RS23955
BWI76_RS23960: BWI76_RS23960 - oxidative stress defense protein, at 5,001,381 to 5,002,148
_RS23960
BWI76_RS23965: BWI76_RS23965 - arginine exporter protein, at 5,002,239 to 5,002,874
_RS23965
Position (kb) 5000
5001
5002 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4999.466 kb on + strand, within BWI76_RS23950 at 4999.603 kb on + strand, within BWI76_RS23950 at 4999.746 kb on - strand, within BWI76_RS23950 at 4999.746 kb on - strand, within BWI76_RS23950 at 4999.850 kb on - strand, within BWI76_RS23950 at 4999.873 kb on + strand, within BWI76_RS23950 at 4999.932 kb on - strand, within BWI76_RS23950 at 4999.980 kb on + strand, within BWI76_RS23950 at 4999.999 kb on + strand, within BWI76_RS23950 at 5000.007 kb on - strand, within BWI76_RS23950 at 5000.146 kb on + strand, within BWI76_RS23950 at 5000.199 kb on - strand, within BWI76_RS23950 at 5000.350 kb on + strand at 5000.350 kb on + strand at 5000.533 kb on - strand, within BWI76_RS23955 at 5000.579 kb on + strand, within BWI76_RS23955 at 5000.624 kb on + strand, within BWI76_RS23955 at 5000.851 kb on + strand, within BWI76_RS23955 at 5000.969 kb on + strand, within BWI76_RS23955 at 5001.080 kb on - strand, within BWI76_RS23955 at 5001.112 kb on + strand, within BWI76_RS23955 at 5001.474 kb on - strand, within BWI76_RS23960 at 5001.603 kb on - strand, within BWI76_RS23960 at 5001.644 kb on - strand, within BWI76_RS23960 at 5001.702 kb on - strand, within BWI76_RS23960 at 5001.769 kb on + strand, within BWI76_RS23960 at 5001.873 kb on - strand, within BWI76_RS23960 at 5001.936 kb on - strand, within BWI76_RS23960 at 5002.184 kb on - strand at 5002.252 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL remove 4,999,466 + BWI76_RS23950 0.39 +0.0 4,999,603 + BWI76_RS23950 0.48 -0.9 4,999,746 - BWI76_RS23950 0.58 -0.8 4,999,746 - BWI76_RS23950 0.58 +0.4 4,999,850 - BWI76_RS23950 0.65 +0.2 4,999,873 + BWI76_RS23950 0.66 -0.5 4,999,932 - BWI76_RS23950 0.70 -2.2 4,999,980 + BWI76_RS23950 0.74 -0.2 4,999,999 + BWI76_RS23950 0.75 +0.4 5,000,007 - BWI76_RS23950 0.75 +0.4 5,000,146 + BWI76_RS23950 0.85 -3.1 5,000,199 - BWI76_RS23950 0.89 +0.4 5,000,350 + -0.7 5,000,350 + -0.5 5,000,533 - BWI76_RS23955 0.19 +1.5 5,000,579 + BWI76_RS23955 0.24 +1.0 5,000,624 + BWI76_RS23955 0.29 +0.3 5,000,851 + BWI76_RS23955 0.54 +0.6 5,000,969 + BWI76_RS23955 0.68 +0.1 5,001,080 - BWI76_RS23955 0.80 -2.1 5,001,112 + BWI76_RS23955 0.84 +0.5 5,001,474 - BWI76_RS23960 0.12 -0.1 5,001,603 - BWI76_RS23960 0.29 -0.3 5,001,644 - BWI76_RS23960 0.34 -0.0 5,001,702 - BWI76_RS23960 0.42 +2.7 5,001,769 + BWI76_RS23960 0.51 +0.9 5,001,873 - BWI76_RS23960 0.64 -1.7 5,001,936 - BWI76_RS23960 0.72 +0.8 5,002,184 - +0.4 5,002,252 + -1.1
Or see this region's nucleotide sequence