Experiment: LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20270 and BWI76_RS20275 are separated by 2 nucleotides BWI76_RS20275 and BWI76_RS20280 are separated by 58 nucleotides BWI76_RS20280 and BWI76_RS20285 are separated by 56 nucleotides BWI76_RS20285 and BWI76_RS20290 are separated by 208 nucleotides
BWI76_RS20270: BWI76_RS20270 - hypothetical protein, at 4,248,064 to 4,249,083
_RS20270
BWI76_RS20275: BWI76_RS20275 - NUDIX hydrolase, at 4,249,086 to 4,249,640
_RS20275
BWI76_RS20280: BWI76_RS20280 - phosphodiesterase, at 4,249,699 to 4,250,250
_RS20280
BWI76_RS20285: BWI76_RS20285 - glutathione S-transferase, at 4,250,307 to 4,250,951
_RS20285
BWI76_RS20290: BWI76_RS20290 - thiol:disulfide oxidoreductase, at 4,251,160 to 4,251,789
_RS20290
Position (kb) 4249
4250
4251 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4248.726 kb on - strand, within BWI76_RS20270 at 4248.732 kb on + strand, within BWI76_RS20270 at 4248.845 kb on + strand, within BWI76_RS20270 at 4248.908 kb on + strand at 4249.402 kb on - strand, within BWI76_RS20275 at 4249.673 kb on - strand at 4249.776 kb on + strand, within BWI76_RS20280 at 4249.885 kb on - strand, within BWI76_RS20280 at 4249.922 kb on + strand, within BWI76_RS20280 at 4250.187 kb on + strand, within BWI76_RS20280 at 4250.187 kb on + strand, within BWI76_RS20280 at 4250.226 kb on - strand at 4250.372 kb on - strand, within BWI76_RS20285 at 4250.639 kb on - strand, within BWI76_RS20285 at 4250.849 kb on + strand, within BWI76_RS20285 at 4250.946 kb on + strand at 4251.047 kb on + strand at 4251.055 kb on - strand at 4251.062 kb on - strand at 4251.082 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL remove 4,248,726 - BWI76_RS20270 0.65 -0.3 4,248,732 + BWI76_RS20270 0.65 +0.9 4,248,845 + BWI76_RS20270 0.77 -0.4 4,248,908 + +2.5 4,249,402 - BWI76_RS20275 0.57 -0.7 4,249,673 - +0.3 4,249,776 + BWI76_RS20280 0.14 +1.2 4,249,885 - BWI76_RS20280 0.34 -1.7 4,249,922 + BWI76_RS20280 0.40 -1.4 4,250,187 + BWI76_RS20280 0.88 +1.2 4,250,187 + BWI76_RS20280 0.88 -2.0 4,250,226 - +0.5 4,250,372 - BWI76_RS20285 0.10 +0.2 4,250,639 - BWI76_RS20285 0.51 -0.7 4,250,849 + BWI76_RS20285 0.84 -1.3 4,250,946 + +0.3 4,251,047 + +1.1 4,251,055 - -0.3 4,251,062 - +0.2 4,251,082 + +0.2
Or see this region's nucleotide sequence