Experiment: LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS19235 and BWI76_RS19240 are separated by 64 nucleotides BWI76_RS19240 and BWI76_RS19245 are separated by 27 nucleotides BWI76_RS19245 and BWI76_RS19250 are separated by 384 nucleotides
BWI76_RS19235: BWI76_RS19235 - polar amino acid ABC transporter inner membrane subunit, at 4,002,394 to 4,003,086
_RS19235
BWI76_RS19240: BWI76_RS19240 - ABC transporter substrate-binding protein, at 4,003,151 to 4,003,924
_RS19240
BWI76_RS19245: BWI76_RS19245 - dimethylargininase, at 4,003,952 to 4,004,713
_RS19245
BWI76_RS19250: BWI76_RS19250 - multidrug transporter subunit MdtA, at 4,005,098 to 4,006,342
_RS19250
Position (kb) 4003
4004
4005 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 4003.014 kb on + strand, within BWI76_RS19235 at 4003.060 kb on + strand at 4003.221 kb on + strand at 4003.403 kb on - strand, within BWI76_RS19240 at 4003.893 kb on - strand at 4003.973 kb on + strand at 4004.152 kb on - strand, within BWI76_RS19245 at 4004.159 kb on - strand, within BWI76_RS19245 at 4004.271 kb on - strand, within BWI76_RS19245 at 4004.273 kb on + strand, within BWI76_RS19245 at 4004.588 kb on + strand, within BWI76_RS19245 at 4004.662 kb on - strand at 4004.741 kb on + strand at 4004.805 kb on - strand at 4004.994 kb on + strand at 4005.307 kb on - strand, within BWI76_RS19250 at 4005.364 kb on - strand, within BWI76_RS19250 at 4005.514 kb on - strand, within BWI76_RS19250
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL remove 4,003,014 + BWI76_RS19235 0.89 -1.1 4,003,060 + -0.2 4,003,221 + +0.4 4,003,403 - BWI76_RS19240 0.33 +1.2 4,003,893 - +0.0 4,003,973 + +0.2 4,004,152 - BWI76_RS19245 0.26 +0.5 4,004,159 - BWI76_RS19245 0.27 +3.4 4,004,271 - BWI76_RS19245 0.42 +0.3 4,004,273 + BWI76_RS19245 0.42 -2.4 4,004,588 + BWI76_RS19245 0.83 -0.7 4,004,662 - +0.5 4,004,741 + -1.7 4,004,805 - +0.3 4,004,994 + -1.7 4,005,307 - BWI76_RS19250 0.17 +0.1 4,005,364 - BWI76_RS19250 0.21 +0.7 4,005,514 - BWI76_RS19250 0.33 +1.0
Or see this region's nucleotide sequence