Experiment: LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS15905 and BWI76_RS15910 are separated by 60 nucleotides BWI76_RS15910 and BWI76_RS15915 overlap by 7 nucleotides BWI76_RS15915 and BWI76_RS15920 are separated by 102 nucleotides BWI76_RS15920 and BWI76_RS15925 are separated by 148 nucleotides
BWI76_RS15905: BWI76_RS15905 - hypothetical protein, at 3,300,090 to 3,300,431
_RS15905
BWI76_RS15910: BWI76_RS15910 - spermidine acetyltransferase, at 3,300,492 to 3,301,052
_RS15910
BWI76_RS15915: BWI76_RS15915 - YnfC family lipoprotein, at 3,301,046 to 3,301,756
_RS15915
BWI76_RS15920: BWI76_RS15920 - hypothetical protein, at 3,301,859 to 3,302,110
_RS15920
BWI76_RS15925: BWI76_RS15925 - dimethyl sulfoxide reductase subunit A, at 3,302,259 to 3,304,694
_RS15925
Position (kb)
3301
3302 Strain fitness (log2 ratio)
-2
-1
0
1 at 3300.394 kb on + strand, within BWI76_RS15905 at 3300.402 kb on - strand at 3300.402 kb on - strand at 3300.545 kb on - strand at 3300.545 kb on - strand at 3300.847 kb on + strand, within BWI76_RS15910 at 3301.015 kb on - strand at 3301.038 kb on + strand at 3301.082 kb on - strand at 3301.114 kb on - strand at 3301.123 kb on + strand, within BWI76_RS15915 at 3301.234 kb on + strand, within BWI76_RS15915 at 3301.677 kb on + strand, within BWI76_RS15915 at 3301.689 kb on + strand at 3301.702 kb on + strand at 3301.779 kb on - strand at 3301.871 kb on + strand at 3301.887 kb on - strand, within BWI76_RS15920 at 3302.134 kb on - strand at 3302.473 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL remove 3,300,394 + BWI76_RS15905 0.89 +1.2 3,300,402 - -0.4 3,300,402 - -2.3 3,300,545 - +0.7 3,300,545 - +0.3 3,300,847 + BWI76_RS15910 0.63 -0.4 3,301,015 - +0.3 3,301,038 + +1.2 3,301,082 - +1.2 3,301,114 - +0.3 3,301,123 + BWI76_RS15915 0.11 +0.5 3,301,234 + BWI76_RS15915 0.26 +1.6 3,301,677 + BWI76_RS15915 0.89 -0.0 3,301,689 + -1.2 3,301,702 + +0.5 3,301,779 - +0.2 3,301,871 + -1.3 3,301,887 - BWI76_RS15920 0.11 -0.5 3,302,134 - -1.3 3,302,473 - +1.1
Or see this region's nucleotide sequence