Experiment: LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12385 and BWI76_RS12390 are separated by 135 nucleotides BWI76_RS12390 and BWI76_RS12395 are separated by 349 nucleotides BWI76_RS12395 and BWI76_RS12400 are separated by 59 nucleotides
BWI76_RS12385: BWI76_RS12385 - DNA polymerase V subunit UmuC, at 2,586,868 to 2,588,133
_RS12385
BWI76_RS12390: BWI76_RS12390 - antibiotic biosynthesis monooxygenase, at 2,588,269 to 2,588,568
_RS12390
BWI76_RS12395: BWI76_RS12395 - zinc-binding alcohol dehydrogenase, at 2,588,918 to 2,589,901
_RS12395
BWI76_RS12400: BWI76_RS12400 - TetR family transcriptional regulator, at 2,589,961 to 2,590,617
_RS12400
Position (kb)
2588
2589
2590 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2587.963 kb on + strand, within BWI76_RS12385 at 2588.096 kb on + strand at 2588.104 kb on - strand at 2588.116 kb on - strand at 2588.230 kb on - strand at 2588.365 kb on + strand at 2588.435 kb on + strand, within BWI76_RS12390 at 2588.494 kb on + strand, within BWI76_RS12390 at 2588.624 kb on - strand at 2588.624 kb on - strand at 2588.649 kb on - strand at 2588.675 kb on - strand at 2588.710 kb on + strand at 2588.951 kb on + strand at 2588.994 kb on - strand at 2589.188 kb on - strand, within BWI76_RS12395 at 2589.353 kb on + strand, within BWI76_RS12395 at 2589.381 kb on - strand, within BWI76_RS12395 at 2589.495 kb on + strand, within BWI76_RS12395 at 2589.581 kb on + strand, within BWI76_RS12395 at 2589.605 kb on + strand, within BWI76_RS12395 at 2589.660 kb on + strand, within BWI76_RS12395 at 2589.660 kb on + strand, within BWI76_RS12395 at 2589.660 kb on + strand, within BWI76_RS12395 at 2589.661 kb on - strand, within BWI76_RS12395 at 2589.668 kb on - strand, within BWI76_RS12395 at 2589.678 kb on - strand, within BWI76_RS12395 at 2589.687 kb on + strand, within BWI76_RS12395 at 2589.702 kb on + strand, within BWI76_RS12395 at 2589.708 kb on + strand, within BWI76_RS12395 at 2589.718 kb on + strand, within BWI76_RS12395 at 2589.750 kb on + strand, within BWI76_RS12395 at 2589.781 kb on - strand, within BWI76_RS12395 at 2589.789 kb on + strand, within BWI76_RS12395 at 2589.790 kb on + strand, within BWI76_RS12395 at 2589.797 kb on - strand, within BWI76_RS12395 at 2589.805 kb on + strand at 2589.807 kb on - strand at 2589.810 kb on + strand at 2589.813 kb on + strand at 2589.813 kb on - strand at 2589.815 kb on + strand at 2589.815 kb on - strand at 2589.822 kb on - strand at 2589.834 kb on + strand at 2589.854 kb on + strand at 2589.910 kb on + strand at 2589.910 kb on + strand at 2589.922 kb on + strand at 2589.956 kb on + strand at 2589.956 kb on + strand at 2589.964 kb on + strand at 2589.966 kb on + strand at 2589.972 kb on - strand at 2589.972 kb on - strand at 2589.972 kb on - strand at 2589.974 kb on - strand at 2589.979 kb on - strand at 2589.989 kb on + strand at 2590.005 kb on - strand at 2590.039 kb on - strand, within BWI76_RS12400 at 2590.039 kb on - strand, within BWI76_RS12400 at 2590.044 kb on - strand, within BWI76_RS12400 at 2590.085 kb on - strand, within BWI76_RS12400 at 2590.086 kb on - strand, within BWI76_RS12400 at 2590.201 kb on - strand, within BWI76_RS12400 at 2590.238 kb on - strand, within BWI76_RS12400 at 2590.340 kb on - strand, within BWI76_RS12400 at 2590.353 kb on - strand, within BWI76_RS12400 at 2590.362 kb on - strand at 2590.407 kb on - strand, within BWI76_RS12400 at 2590.524 kb on + strand, within BWI76_RS12400 at 2590.532 kb on - strand, within BWI76_RS12400 at 2590.532 kb on - strand, within BWI76_RS12400 at 2590.532 kb on - strand, within BWI76_RS12400 at 2590.532 kb on - strand, within BWI76_RS12400 at 2590.532 kb on - strand, within BWI76_RS12400 at 2590.613 kb on + strand at 2590.621 kb on - strand at 2590.621 kb on - strand at 2590.679 kb on - strand at 2590.743 kb on + strand at 2590.751 kb on - strand at 2590.751 kb on - strand at 2590.751 kb on - strand at 2590.751 kb on - strand at 2590.757 kb on + strand at 2590.765 kb on - strand at 2590.775 kb on - strand at 2590.775 kb on - strand at 2590.775 kb on - strand at 2590.775 kb on - strand at 2590.779 kb on - strand at 2590.781 kb on - strand at 2590.791 kb on - strand at 2590.801 kb on + strand at 2590.814 kb on + strand at 2590.822 kb on - strand at 2590.822 kb on - strand at 2590.822 kb on - strand at 2590.822 kb on - strand at 2590.822 kb on - strand at 2590.824 kb on + strand at 2590.835 kb on + strand at 2590.859 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL remove 2,587,963 + BWI76_RS12385 0.86 +0.2 2,588,096 + -0.0 2,588,104 - +0.2 2,588,116 - -2.5 2,588,230 - -2.2 2,588,365 + -1.4 2,588,435 + BWI76_RS12390 0.55 +0.1 2,588,494 + BWI76_RS12390 0.75 -0.4 2,588,624 - -2.2 2,588,624 - +0.1 2,588,649 - -2.9 2,588,675 - -1.1 2,588,710 + +0.5 2,588,951 + -1.8 2,588,994 - -0.4 2,589,188 - BWI76_RS12395 0.27 -3.1 2,589,353 + BWI76_RS12395 0.44 -1.5 2,589,381 - BWI76_RS12395 0.47 -1.6 2,589,495 + BWI76_RS12395 0.59 -0.6 2,589,581 + BWI76_RS12395 0.67 -1.4 2,589,605 + BWI76_RS12395 0.70 -0.4 2,589,660 + BWI76_RS12395 0.75 +0.3 2,589,660 + BWI76_RS12395 0.75 -0.8 2,589,660 + BWI76_RS12395 0.75 -0.6 2,589,661 - BWI76_RS12395 0.76 -0.9 2,589,668 - BWI76_RS12395 0.76 -1.8 2,589,678 - BWI76_RS12395 0.77 -3.2 2,589,687 + BWI76_RS12395 0.78 -1.3 2,589,702 + BWI76_RS12395 0.80 -0.4 2,589,708 + BWI76_RS12395 0.80 -0.5 2,589,718 + BWI76_RS12395 0.81 -1.4 2,589,750 + BWI76_RS12395 0.85 -2.8 2,589,781 - BWI76_RS12395 0.88 -0.8 2,589,789 + BWI76_RS12395 0.89 -1.8 2,589,790 + BWI76_RS12395 0.89 -2.5 2,589,797 - BWI76_RS12395 0.89 +0.5 2,589,805 + +0.1 2,589,807 - +1.4 2,589,810 + +0.8 2,589,813 + -0.6 2,589,813 - -2.5 2,589,815 + -0.2 2,589,815 - +1.8 2,589,822 - -2.3 2,589,834 + +0.2 2,589,854 + -0.6 2,589,910 + -3.1 2,589,910 + -0.1 2,589,922 + -1.0 2,589,956 + -0.4 2,589,956 + -0.4 2,589,964 + +1.2 2,589,966 + -0.8 2,589,972 - +0.3 2,589,972 - -0.6 2,589,972 - -1.1 2,589,974 - -0.0 2,589,979 - -0.2 2,589,989 + -1.1 2,590,005 - -0.9 2,590,039 - BWI76_RS12400 0.12 -0.6 2,590,039 - BWI76_RS12400 0.12 -1.1 2,590,044 - BWI76_RS12400 0.13 -0.2 2,590,085 - BWI76_RS12400 0.19 -1.6 2,590,086 - BWI76_RS12400 0.19 -0.3 2,590,201 - BWI76_RS12400 0.37 -0.8 2,590,238 - BWI76_RS12400 0.42 -2.4 2,590,340 - BWI76_RS12400 0.58 -1.1 2,590,353 - BWI76_RS12400 0.60 -2.3 2,590,362 - +0.1 2,590,407 - BWI76_RS12400 0.68 +0.4 2,590,524 + BWI76_RS12400 0.86 -0.8 2,590,532 - BWI76_RS12400 0.87 -1.8 2,590,532 - BWI76_RS12400 0.87 +0.2 2,590,532 - BWI76_RS12400 0.87 +1.2 2,590,532 - BWI76_RS12400 0.87 +0.6 2,590,532 - BWI76_RS12400 0.87 +2.5 2,590,613 + -3.1 2,590,621 - +1.2 2,590,621 - -0.9 2,590,679 - -1.2 2,590,743 + -0.4 2,590,751 - -0.8 2,590,751 - +0.7 2,590,751 - -2.1 2,590,751 - -2.1 2,590,757 + -1.4 2,590,765 - -0.8 2,590,775 - +1.1 2,590,775 - -2.5 2,590,775 - -1.1 2,590,775 - -0.3 2,590,779 - +1.2 2,590,781 - -0.7 2,590,791 - +0.2 2,590,801 + -0.8 2,590,814 + -1.6 2,590,822 - -3.1 2,590,822 - -1.4 2,590,822 - +0.7 2,590,822 - +0.2 2,590,822 - -1.1 2,590,824 + -0.8 2,590,835 + -2.1 2,590,859 + +0.5
Or see this region's nucleotide sequence