Experiment: LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02265 and BWI76_RS02270 are separated by 224 nucleotides BWI76_RS02270 and BWI76_RS02275 are separated by 36 nucleotides BWI76_RS02275 and BWI76_RS02280 are separated by 115 nucleotides
BWI76_RS02265: BWI76_RS02265 - putative transmembrane protein, at 465,855 to 466,535
_RS02265
BWI76_RS02270: BWI76_RS02270 - DSBA oxidoreductase, at 466,760 to 467,542
_RS02270
BWI76_RS02275: BWI76_RS02275 - TetR family transcriptional regulator, at 467,579 to 468,175
_RS02275
BWI76_RS02280: BWI76_RS02280 - NAD(P)H dehydrogenase (quinone), at 468,291 to 468,878
_RS02280
Position (kb)
466
467
468 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 465.801 kb on - strand at 465.853 kb on + strand at 465.907 kb on - strand at 465.907 kb on - strand at 465.925 kb on - strand, within BWI76_RS02265 at 466.201 kb on - strand, within BWI76_RS02265 at 466.315 kb on - strand, within BWI76_RS02265 at 466.421 kb on + strand, within BWI76_RS02265 at 466.429 kb on - strand, within BWI76_RS02265 at 466.434 kb on - strand, within BWI76_RS02265 at 466.589 kb on + strand at 466.589 kb on + strand at 466.589 kb on + strand at 466.591 kb on - strand at 466.597 kb on - strand at 466.606 kb on - strand at 466.614 kb on + strand at 466.695 kb on + strand at 466.740 kb on - strand at 466.859 kb on + strand, within BWI76_RS02270 at 466.926 kb on - strand, within BWI76_RS02270 at 466.958 kb on + strand, within BWI76_RS02270 at 466.971 kb on - strand, within BWI76_RS02270 at 466.993 kb on - strand, within BWI76_RS02270 at 467.193 kb on - strand, within BWI76_RS02270 at 467.256 kb on - strand, within BWI76_RS02270 at 467.605 kb on - strand at 467.639 kb on - strand, within BWI76_RS02275 at 467.668 kb on - strand, within BWI76_RS02275 at 467.693 kb on + strand, within BWI76_RS02275 at 467.725 kb on + strand at 467.938 kb on - strand, within BWI76_RS02275 at 467.963 kb on - strand, within BWI76_RS02275 at 468.144 kb on - strand at 468.151 kb on - strand at 468.320 kb on + strand at 468.351 kb on - strand, within BWI76_RS02280 at 468.483 kb on - strand, within BWI76_RS02280 at 468.504 kb on - strand, within BWI76_RS02280
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL remove 465,801 - -0.3 465,853 + -0.5 465,907 - +0.6 465,907 - +0.2 465,925 - BWI76_RS02265 0.10 -1.3 466,201 - BWI76_RS02265 0.51 +0.1 466,315 - BWI76_RS02265 0.68 +0.1 466,421 + BWI76_RS02265 0.83 -0.3 466,429 - BWI76_RS02265 0.84 +0.3 466,434 - BWI76_RS02265 0.85 -0.2 466,589 + -1.6 466,589 + -1.3 466,589 + -0.6 466,591 - +2.3 466,597 - -0.2 466,606 - +0.0 466,614 + -2.2 466,695 + -0.3 466,740 - +1.0 466,859 + BWI76_RS02270 0.13 +0.3 466,926 - BWI76_RS02270 0.21 +0.5 466,958 + BWI76_RS02270 0.25 +0.1 466,971 - BWI76_RS02270 0.27 -0.1 466,993 - BWI76_RS02270 0.30 +1.0 467,193 - BWI76_RS02270 0.55 +1.7 467,256 - BWI76_RS02270 0.63 +1.2 467,605 - +0.2 467,639 - BWI76_RS02275 0.10 +0.0 467,668 - BWI76_RS02275 0.15 -0.1 467,693 + BWI76_RS02275 0.19 +0.3 467,725 + +0.0 467,938 - BWI76_RS02275 0.60 -1.0 467,963 - BWI76_RS02275 0.64 +0.8 468,144 - +0.5 468,151 - +1.0 468,320 + +0.4 468,351 - BWI76_RS02280 0.10 +0.7 468,483 - BWI76_RS02280 0.33 +0.6 468,504 - BWI76_RS02280 0.36 +0.4
Or see this region's nucleotide sequence