Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS01090

Experiment: LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS01085 and BWI76_RS01090 are separated by 56 nucleotidesBWI76_RS01090 and BWI76_RS01095 overlap by 4 nucleotides BWI76_RS01085: BWI76_RS01085 - polysaccharide chain length modulation protein, at 223,330 to 224,376 _RS01085 BWI76_RS01090: BWI76_RS01090 - UDP-N-acetylglucosamine 2-epimerase (non-hydrolyzing), at 224,433 to 225,563 _RS01090 BWI76_RS01095: BWI76_RS01095 - UDP-N-acetyl-D-mannosamine dehydrogenase, at 225,560 to 226,822 _RS01095 Position (kb) 224 225 226Strain fitness (log2 ratio) -3 -2 -1 0 1at 223.438 kb on + strand, within BWI76_RS01085at 223.608 kb on + strand, within BWI76_RS01085at 223.697 kb on + strand, within BWI76_RS01085at 223.917 kb on + strand, within BWI76_RS01085at 224.006 kb on + strand, within BWI76_RS01085at 224.069 kb on + strand, within BWI76_RS01085at 224.097 kb on + strand, within BWI76_RS01085at 224.119 kb on - strand, within BWI76_RS01085at 224.184 kb on - strand, within BWI76_RS01085at 224.637 kb on - strand, within BWI76_RS01090at 224.826 kb on - strand, within BWI76_RS01090at 224.859 kb on + strand, within BWI76_RS01090at 225.000 kb on - strand, within BWI76_RS01090at 225.010 kb on + strand, within BWI76_RS01090at 225.020 kb on + strand, within BWI76_RS01090at 225.502 kb on + strandat 225.589 kb on + strandat 226.034 kb on - strand, within BWI76_RS01095at 226.533 kb on + strand, within BWI76_RS01095at 226.541 kb on - strand, within BWI76_RS01095

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Per-strain Table

Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma virens G41 spores at 2.5*10^6 spores/mL
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223,438 + BWI76_RS01085 0.10 -0.3
223,608 + BWI76_RS01085 0.27 +1.0
223,697 + BWI76_RS01085 0.35 -0.6
223,917 + BWI76_RS01085 0.56 -0.6
224,006 + BWI76_RS01085 0.65 -0.5
224,069 + BWI76_RS01085 0.71 -2.3
224,097 + BWI76_RS01085 0.73 +0.0
224,119 - BWI76_RS01085 0.75 +0.0
224,184 - BWI76_RS01085 0.82 -2.6
224,637 - BWI76_RS01090 0.18 +1.0
224,826 - BWI76_RS01090 0.35 +0.1
224,859 + BWI76_RS01090 0.38 -2.0
225,000 - BWI76_RS01090 0.50 -2.1
225,010 + BWI76_RS01090 0.51 -1.3
225,020 + BWI76_RS01090 0.52 -2.2
225,502 + -3.2
225,589 + -0.3
226,034 - BWI76_RS01095 0.38 -2.1
226,533 + BWI76_RS01095 0.77 -2.6
226,541 - BWI76_RS01095 0.78 -1.6

Or see this region's nucleotide sequence