Experiment: D,L-Malic Acid (C) and Ammonium chloride (N); with MOPS; with chloride
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFLU_RS02200 and PFLU_RS02205 are separated by 50 nucleotides PFLU_RS02205 and PFLU_RS02210 are separated by 334 nucleotides
PFLU_RS02200: PFLU_RS02200 - mechanosensitive channel MscK, at 490,640 to 494,023
_RS02200
PFLU_RS02205: PFLU_RS02205 - potassium/proton antiporter, at 494,074 to 495,816
_RS02205
PFLU_RS02210: PFLU_RS02210 - methyl-accepting chemotaxis protein, at 496,151 to 497,776
_RS02210
Position (kb) 494
495
496 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 493.176 kb on + strand, within PFLU_RS02200 at 493.176 kb on + strand, within PFLU_RS02200 at 493.177 kb on - strand, within PFLU_RS02200 at 493.177 kb on - strand, within PFLU_RS02200 at 493.489 kb on + strand, within PFLU_RS02200 at 493.489 kb on + strand, within PFLU_RS02200 at 493.489 kb on + strand, within PFLU_RS02200 at 493.489 kb on + strand, within PFLU_RS02200 at 493.490 kb on - strand, within PFLU_RS02200 at 493.490 kb on - strand, within PFLU_RS02200 at 493.490 kb on - strand, within PFLU_RS02200 at 493.490 kb on - strand, within PFLU_RS02200 at 493.490 kb on - strand, within PFLU_RS02200 at 493.490 kb on - strand, within PFLU_RS02200 at 493.490 kb on - strand, within PFLU_RS02200 at 493.490 kb on - strand, within PFLU_RS02200 at 493.490 kb on - strand, within PFLU_RS02200 at 493.490 kb on - strand, within PFLU_RS02200 at 493.490 kb on - strand, within PFLU_RS02200 at 493.490 kb on - strand, within PFLU_RS02200 at 493.534 kb on + strand, within PFLU_RS02200 at 493.534 kb on + strand, within PFLU_RS02200 at 493.535 kb on - strand, within PFLU_RS02200 at 493.535 kb on - strand, within PFLU_RS02200 at 493.535 kb on - strand, within PFLU_RS02200 at 493.535 kb on - strand, within PFLU_RS02200 at 493.661 kb on + strand, within PFLU_RS02200 at 493.661 kb on + strand, within PFLU_RS02200 at 493.661 kb on + strand, within PFLU_RS02200 at 493.662 kb on - strand, within PFLU_RS02200 at 493.662 kb on - strand, within PFLU_RS02200 at 493.815 kb on + strand at 493.816 kb on - strand at 493.816 kb on - strand at 493.816 kb on - strand at 493.836 kb on + strand at 493.836 kb on + strand at 493.836 kb on + strand at 493.837 kb on - strand at 493.837 kb on - strand at 493.837 kb on - strand at 493.969 kb on + strand at 494.023 kb on + strand at 494.023 kb on + strand at 494.024 kb on - strand at 494.024 kb on - strand at 494.024 kb on - strand at 494.279 kb on + strand, within PFLU_RS02205 at 494.279 kb on + strand, within PFLU_RS02205 at 494.280 kb on - strand, within PFLU_RS02205 at 494.320 kb on + strand, within PFLU_RS02205 at 494.320 kb on + strand, within PFLU_RS02205 at 494.320 kb on + strand, within PFLU_RS02205 at 494.321 kb on - strand, within PFLU_RS02205 at 494.341 kb on + strand, within PFLU_RS02205 at 494.341 kb on + strand, within PFLU_RS02205 at 494.342 kb on - strand, within PFLU_RS02205 at 494.342 kb on - strand, within PFLU_RS02205 at 494.342 kb on - strand, within PFLU_RS02205 at 494.445 kb on + strand, within PFLU_RS02205 at 494.445 kb on + strand, within PFLU_RS02205 at 494.446 kb on - strand, within PFLU_RS02205 at 494.446 kb on - strand, within PFLU_RS02205 at 494.446 kb on - strand, within PFLU_RS02205 at 494.446 kb on - strand, within PFLU_RS02205 at 494.448 kb on + strand, within PFLU_RS02205 at 494.449 kb on - strand, within PFLU_RS02205 at 494.639 kb on - strand, within PFLU_RS02205 at 494.710 kb on - strand, within PFLU_RS02205 at 494.729 kb on + strand, within PFLU_RS02205 at 494.729 kb on + strand, within PFLU_RS02205 at 494.729 kb on + strand, within PFLU_RS02205 at 494.729 kb on + strand, within PFLU_RS02205 at 494.730 kb on - strand, within PFLU_RS02205 at 494.730 kb on - strand, within PFLU_RS02205 at 494.730 kb on - strand, within PFLU_RS02205 at 494.730 kb on - strand, within PFLU_RS02205 at 494.730 kb on - strand, within PFLU_RS02205 at 494.949 kb on + strand, within PFLU_RS02205 at 494.949 kb on + strand, within PFLU_RS02205 at 494.950 kb on - strand, within PFLU_RS02205 at 495.012 kb on + strand, within PFLU_RS02205 at 495.013 kb on - strand, within PFLU_RS02205 at 495.071 kb on + strand, within PFLU_RS02205 at 495.071 kb on + strand, within PFLU_RS02205 at 495.072 kb on - strand, within PFLU_RS02205 at 495.072 kb on - strand, within PFLU_RS02205 at 495.153 kb on - strand, within PFLU_RS02205 at 495.465 kb on + strand, within PFLU_RS02205 at 495.514 kb on - strand, within PFLU_RS02205 at 495.604 kb on + strand, within PFLU_RS02205 at 495.604 kb on + strand, within PFLU_RS02205 at 495.604 kb on + strand, within PFLU_RS02205 at 495.605 kb on - strand, within PFLU_RS02205 at 495.605 kb on - strand, within PFLU_RS02205 at 495.605 kb on - strand, within PFLU_RS02205 at 495.635 kb on + strand, within PFLU_RS02205 at 495.636 kb on - strand, within PFLU_RS02205 at 495.636 kb on - strand, within PFLU_RS02205 at 495.636 kb on - strand, within PFLU_RS02205 at 495.636 kb on - strand, within PFLU_RS02205 at 495.647 kb on + strand at 495.647 kb on + strand at 495.647 kb on + strand at 495.648 kb on - strand at 495.648 kb on - strand at 495.648 kb on - strand at 495.651 kb on + strand at 495.652 kb on - strand at 495.748 kb on + strand at 495.748 kb on + strand at 495.748 kb on + strand at 495.749 kb on - strand at 495.749 kb on - strand at 495.749 kb on - strand at 495.823 kb on + strand at 495.823 kb on + strand at 495.824 kb on - strand at 495.824 kb on - strand at 495.824 kb on - strand at 495.884 kb on + strand at 495.884 kb on + strand at 495.884 kb on + strand at 495.884 kb on + strand at 495.884 kb on + strand at 495.884 kb on + strand at 495.885 kb on - strand at 495.885 kb on - strand at 495.885 kb on - strand at 495.885 kb on - strand at 495.885 kb on - strand at 495.885 kb on - strand at 496.058 kb on + strand at 496.058 kb on + strand at 496.058 kb on + strand at 496.058 kb on + strand at 496.058 kb on + strand at 496.058 kb on + strand at 496.059 kb on - strand at 496.059 kb on - strand at 496.059 kb on - strand at 496.059 kb on - strand at 496.059 kb on - strand at 496.059 kb on - strand at 496.059 kb on - strand at 496.059 kb on - strand at 496.064 kb on + strand at 496.153 kb on - strand at 496.153 kb on - strand at 496.169 kb on + strand at 496.169 kb on + strand at 496.170 kb on - strand at 496.250 kb on + strand at 496.250 kb on + strand at 496.715 kb on + strand, within PFLU_RS02210 at 496.715 kb on + strand, within PFLU_RS02210 at 496.715 kb on + strand, within PFLU_RS02210 at 496.715 kb on + strand, within PFLU_RS02210 at 496.715 kb on + strand, within PFLU_RS02210 at 496.715 kb on + strand, within PFLU_RS02210 at 496.716 kb on - strand, within PFLU_RS02210 at 496.716 kb on - strand, within PFLU_RS02210 at 496.716 kb on - strand, within PFLU_RS02210 at 496.716 kb on - strand, within PFLU_RS02210
Per-strain Table
Position Strand Gene LocusTag Fraction D,L-Malic Acid (C) and Ammonium chloride (N); with MOPS; with chloride remove 493,176 + PFLU_RS02200 0.75 +1.2 493,176 + PFLU_RS02200 0.75 -1.1 493,177 - PFLU_RS02200 0.75 -1.4 493,177 - PFLU_RS02200 0.75 -1.1 493,489 + PFLU_RS02200 0.84 +1.8 493,489 + PFLU_RS02200 0.84 +0.6 493,489 + PFLU_RS02200 0.84 +1.8 493,489 + PFLU_RS02200 0.84 -2.4 493,490 - PFLU_RS02200 0.84 -0.6 493,490 - PFLU_RS02200 0.84 -0.2 493,490 - PFLU_RS02200 0.84 +1.3 493,490 - PFLU_RS02200 0.84 -1.1 493,490 - PFLU_RS02200 0.84 +2.8 493,490 - PFLU_RS02200 0.84 -0.8 493,490 - PFLU_RS02200 0.84 -0.8 493,490 - PFLU_RS02200 0.84 -0.7 493,490 - PFLU_RS02200 0.84 -0.7 493,490 - PFLU_RS02200 0.84 +1.0 493,490 - PFLU_RS02200 0.84 +1.3 493,490 - PFLU_RS02200 0.84 +1.1 493,534 + PFLU_RS02200 0.86 -0.4 493,534 + PFLU_RS02200 0.86 -1.6 493,535 - PFLU_RS02200 0.86 +0.8 493,535 - PFLU_RS02200 0.86 +1.2 493,535 - PFLU_RS02200 0.86 +1.2 493,535 - PFLU_RS02200 0.86 -1.3 493,661 + PFLU_RS02200 0.89 -1.6 493,661 + PFLU_RS02200 0.89 -2.1 493,661 + PFLU_RS02200 0.89 +1.2 493,662 - PFLU_RS02200 0.89 +4.3 493,662 - PFLU_RS02200 0.89 +1.5 493,815 + +3.6 493,816 - -1.5 493,816 - +0.8 493,816 - -0.2 493,836 + -0.6 493,836 + +2.1 493,836 + +1.8 493,837 - -0.0 493,837 - +0.4 493,837 - -1.6 493,969 + +1.2 494,023 + +1.6 494,023 + -0.6 494,024 - -0.0 494,024 - -1.6 494,024 - +1.8 494,279 + PFLU_RS02205 0.12 +0.4 494,279 + PFLU_RS02205 0.12 -1.9 494,280 - PFLU_RS02205 0.12 +1.6 494,320 + PFLU_RS02205 0.14 -1.4 494,320 + PFLU_RS02205 0.14 +1.5 494,320 + PFLU_RS02205 0.14 -1.9 494,321 - PFLU_RS02205 0.14 -2.0 494,341 + PFLU_RS02205 0.15 -1.8 494,341 + PFLU_RS02205 0.15 -0.0 494,342 - PFLU_RS02205 0.15 -0.0 494,342 - PFLU_RS02205 0.15 +1.2 494,342 - PFLU_RS02205 0.15 -0.7 494,445 + PFLU_RS02205 0.21 -1.3 494,445 + PFLU_RS02205 0.21 -0.4 494,446 - PFLU_RS02205 0.21 +1.5 494,446 - PFLU_RS02205 0.21 +0.6 494,446 - PFLU_RS02205 0.21 +0.2 494,446 - PFLU_RS02205 0.21 -1.6 494,448 + PFLU_RS02205 0.21 +0.8 494,449 - PFLU_RS02205 0.22 -1.2 494,639 - PFLU_RS02205 0.32 -1.0 494,710 - PFLU_RS02205 0.36 -0.0 494,729 + PFLU_RS02205 0.38 -1.9 494,729 + PFLU_RS02205 0.38 +0.2 494,729 + PFLU_RS02205 0.38 -0.9 494,729 + PFLU_RS02205 0.38 -0.8 494,730 - PFLU_RS02205 0.38 -1.9 494,730 - PFLU_RS02205 0.38 -0.0 494,730 - PFLU_RS02205 0.38 -1.0 494,730 - PFLU_RS02205 0.38 -2.9 494,730 - PFLU_RS02205 0.38 +1.2 494,949 + PFLU_RS02205 0.50 -0.9 494,949 + PFLU_RS02205 0.50 +0.4 494,950 - PFLU_RS02205 0.50 -1.3 495,012 + PFLU_RS02205 0.54 +0.2 495,013 - PFLU_RS02205 0.54 +1.6 495,071 + PFLU_RS02205 0.57 -2.2 495,071 + PFLU_RS02205 0.57 +2.8 495,072 - PFLU_RS02205 0.57 +0.8 495,072 - PFLU_RS02205 0.57 -2.7 495,153 - PFLU_RS02205 0.62 -0.0 495,465 + PFLU_RS02205 0.80 -2.0 495,514 - PFLU_RS02205 0.83 -1.7 495,604 + PFLU_RS02205 0.88 -1.8 495,604 + PFLU_RS02205 0.88 -1.2 495,604 + PFLU_RS02205 0.88 +1.7 495,605 - PFLU_RS02205 0.88 -0.8 495,605 - PFLU_RS02205 0.88 +0.9 495,605 - PFLU_RS02205 0.88 -2.1 495,635 + PFLU_RS02205 0.90 -1.6 495,636 - PFLU_RS02205 0.90 -2.8 495,636 - PFLU_RS02205 0.90 -0.4 495,636 - PFLU_RS02205 0.90 -0.0 495,636 - PFLU_RS02205 0.90 -1.1 495,647 + +0.5 495,647 + +2.1 495,647 + +2.1 495,648 - +0.7 495,648 - +1.8 495,648 - -1.6 495,651 + +0.2 495,652 - +2.0 495,748 + +3.3 495,748 + +0.6 495,748 + -1.0 495,749 - -0.2 495,749 - -1.6 495,749 - -2.1 495,823 + +0.8 495,823 + -0.8 495,824 - -2.1 495,824 - +2.4 495,824 - -1.1 495,884 + -1.1 495,884 + -1.5 495,884 + -0.6 495,884 + +0.9 495,884 + +0.8 495,884 + +0.6 495,885 - -0.2 495,885 - -1.4 495,885 - -0.2 495,885 - +0.1 495,885 - +3.2 495,885 - +2.3 496,058 + +1.1 496,058 + -0.9 496,058 + -1.3 496,058 + +1.3 496,058 + -0.2 496,058 + +1.0 496,059 - -1.4 496,059 - +2.7 496,059 - +1.5 496,059 - -2.7 496,059 - -2.3 496,059 - +1.2 496,059 - +0.3 496,059 - -1.6 496,064 + +1.8 496,153 - +1.9 496,153 - -0.2 496,169 + +2.2 496,169 + -1.1 496,170 - -1.4 496,250 + +2.8 496,250 + -1.8 496,715 + PFLU_RS02210 0.35 -1.3 496,715 + PFLU_RS02210 0.35 +1.1 496,715 + PFLU_RS02210 0.35 +1.1 496,715 + PFLU_RS02210 0.35 -0.8 496,715 + PFLU_RS02210 0.35 -0.4 496,715 + PFLU_RS02210 0.35 +1.7 496,716 - PFLU_RS02210 0.35 -1.2 496,716 - PFLU_RS02210 0.35 +3.8 496,716 - PFLU_RS02210 0.35 -2.1 496,716 - PFLU_RS02210 0.35 +1.9
Or see this region's nucleotide sequence