Experiment: LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12305 and BWI76_RS12310 are separated by 137 nucleotides BWI76_RS12310 and BWI76_RS12315 are separated by 507 nucleotides BWI76_RS12315 and BWI76_RS12320 are separated by 13 nucleotides BWI76_RS12320 and BWI76_RS12325 are separated by 93 nucleotides
BWI76_RS12305: BWI76_RS12305 - hypothetical protein, at 2,568,592 to 2,569,074
_RS12305
BWI76_RS12310: BWI76_RS12310 - hypothetical protein, at 2,569,212 to 2,569,427
_RS12310
BWI76_RS12315: BWI76_RS12315 - hypothetical protein, at 2,569,935 to 2,570,459
_RS12315
BWI76_RS12320: BWI76_RS12320 - hypothetical protein, at 2,570,473 to 2,570,847
_RS12320
BWI76_RS12325: BWI76_RS12325 - hypothetical protein, at 2,570,941 to 2,574,228
_RS12325
Position (kb)
2569
2570
2571 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2568.937 kb on + strand, within BWI76_RS12305 at 2569.001 kb on - strand, within BWI76_RS12305 at 2569.032 kb on + strand at 2569.040 kb on - strand at 2569.087 kb on + strand at 2569.432 kb on + strand at 2569.459 kb on + strand at 2569.459 kb on + strand at 2569.467 kb on - strand at 2569.467 kb on - strand at 2569.467 kb on - strand at 2569.485 kb on + strand at 2569.485 kb on - strand at 2569.490 kb on + strand at 2569.490 kb on + strand at 2569.490 kb on + strand at 2569.498 kb on - strand at 2569.498 kb on - strand at 2569.733 kb on + strand at 2569.742 kb on - strand at 2569.760 kb on + strand at 2569.778 kb on + strand at 2569.868 kb on + strand at 2569.931 kb on + strand at 2569.937 kb on + strand at 2569.937 kb on + strand at 2570.305 kb on - strand, within BWI76_RS12315 at 2570.404 kb on + strand, within BWI76_RS12315 at 2570.421 kb on + strand at 2570.423 kb on + strand at 2570.423 kb on + strand at 2570.497 kb on + strand at 2570.507 kb on + strand at 2570.534 kb on - strand, within BWI76_RS12320 at 2570.587 kb on + strand, within BWI76_RS12320 at 2570.592 kb on + strand, within BWI76_RS12320 at 2570.605 kb on - strand, within BWI76_RS12320 at 2570.611 kb on + strand, within BWI76_RS12320 at 2570.624 kb on + strand, within BWI76_RS12320 at 2570.624 kb on + strand, within BWI76_RS12320 at 2570.634 kb on + strand, within BWI76_RS12320 at 2570.647 kb on + strand, within BWI76_RS12320 at 2570.676 kb on + strand, within BWI76_RS12320 at 2570.676 kb on + strand, within BWI76_RS12320 at 2570.680 kb on + strand at 2570.697 kb on + strand, within BWI76_RS12320 at 2570.700 kb on + strand, within BWI76_RS12320 at 2570.701 kb on + strand, within BWI76_RS12320 at 2570.707 kb on + strand, within BWI76_RS12320 at 2570.707 kb on + strand, within BWI76_RS12320 at 2570.707 kb on + strand, within BWI76_RS12320 at 2570.707 kb on + strand, within BWI76_RS12320 at 2570.745 kb on + strand, within BWI76_RS12320 at 2570.848 kb on + strand at 2570.971 kb on + strand at 2570.979 kb on - strand at 2570.979 kb on - strand at 2571.009 kb on + strand at 2571.051 kb on - strand at 2571.059 kb on - strand at 2571.103 kb on + strand at 2571.106 kb on - strand at 2571.157 kb on - strand at 2571.208 kb on + strand at 2571.302 kb on + strand, within BWI76_RS12325 at 2571.310 kb on - strand, within BWI76_RS12325 at 2571.368 kb on + strand at 2571.393 kb on + strand, within BWI76_RS12325
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL remove 2,568,937 + BWI76_RS12305 0.71 -1.5 2,569,001 - BWI76_RS12305 0.85 -0.3 2,569,032 + -1.8 2,569,040 - -1.7 2,569,087 + +0.2 2,569,432 + -1.9 2,569,459 + -0.4 2,569,459 + -2.3 2,569,467 - -2.6 2,569,467 - +1.2 2,569,467 - -0.8 2,569,485 + -1.1 2,569,485 - +1.9 2,569,490 + -1.7 2,569,490 + -0.7 2,569,490 + -1.5 2,569,498 - -0.5 2,569,498 - -0.4 2,569,733 + -0.7 2,569,742 - -0.7 2,569,760 + -2.3 2,569,778 + +0.9 2,569,868 + -0.4 2,569,931 + +0.1 2,569,937 + -1.9 2,569,937 + -1.4 2,570,305 - BWI76_RS12315 0.70 -1.4 2,570,404 + BWI76_RS12315 0.89 -1.1 2,570,421 + -1.7 2,570,423 + +0.9 2,570,423 + +0.9 2,570,497 + -1.4 2,570,507 + +1.6 2,570,534 - BWI76_RS12320 0.16 -0.7 2,570,587 + BWI76_RS12320 0.30 +0.2 2,570,592 + BWI76_RS12320 0.32 -2.6 2,570,605 - BWI76_RS12320 0.35 +0.9 2,570,611 + BWI76_RS12320 0.37 -2.9 2,570,624 + BWI76_RS12320 0.40 -1.3 2,570,624 + BWI76_RS12320 0.40 -0.9 2,570,634 + BWI76_RS12320 0.43 -0.7 2,570,647 + BWI76_RS12320 0.46 -0.1 2,570,676 + BWI76_RS12320 0.54 -0.2 2,570,676 + BWI76_RS12320 0.54 -0.1 2,570,680 + -0.1 2,570,697 + BWI76_RS12320 0.60 -0.6 2,570,700 + BWI76_RS12320 0.61 -0.7 2,570,701 + BWI76_RS12320 0.61 -1.5 2,570,707 + BWI76_RS12320 0.62 -0.7 2,570,707 + BWI76_RS12320 0.62 -1.5 2,570,707 + BWI76_RS12320 0.62 -0.8 2,570,707 + BWI76_RS12320 0.62 -0.8 2,570,745 + BWI76_RS12320 0.73 -1.1 2,570,848 + -1.4 2,570,971 + -1.4 2,570,979 - -0.7 2,570,979 - -0.2 2,571,009 + +0.7 2,571,051 - -1.1 2,571,059 - -1.7 2,571,103 + -0.3 2,571,106 - -1.7 2,571,157 - +1.7 2,571,208 + -1.0 2,571,302 + BWI76_RS12325 0.11 -0.5 2,571,310 - BWI76_RS12325 0.11 -1.4 2,571,368 + -1.1 2,571,393 + BWI76_RS12325 0.14 -1.9
Or see this region's nucleotide sequence