Experiment: LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12020 and BWI76_RS12025 are separated by 134 nucleotides BWI76_RS12025 and BWI76_RS12030 overlap by 104 nucleotides BWI76_RS12030 and BWI76_RS12035 are separated by 9 nucleotides BWI76_RS12035 and BWI76_RS12040 are separated by 7 nucleotides BWI76_RS12040 and BWI76_RS12045 overlap by 4 nucleotides
BWI76_RS12020: BWI76_RS12020 - antimicrobial peptide ABC transporter permease SapC, at 2,528,944 to 2,529,834
_RS12020
BWI76_RS12025: BWI76_RS12025 - integrase, at 2,529,969 to 2,531,198
_RS12025
BWI76_RS12030: BWI76_RS12030 - hypothetical protein, at 2,531,095 to 2,531,409
_RS12030
BWI76_RS12035: BWI76_RS12035 - hypothetical protein, at 2,531,419 to 2,531,658
_RS12035
BWI76_RS12040: BWI76_RS12040 - hypothetical protein, at 2,531,666 to 2,531,974
_RS12040
BWI76_RS12045: BWI76_RS12045 - hypothetical protein, at 2,531,971 to 2,532,690
_RS12045
Position (kb)
2529
2530
2531
2532 Strain fitness (log2 ratio)
-1
0
1 at 2529.985 kb on + strand at 2530.010 kb on - strand at 2530.010 kb on - strand at 2530.010 kb on - strand at 2530.125 kb on - strand, within BWI76_RS12025 at 2530.170 kb on + strand, within BWI76_RS12025 at 2530.269 kb on - strand, within BWI76_RS12025 at 2530.458 kb on - strand at 2530.504 kb on + strand, within BWI76_RS12025 at 2530.504 kb on + strand, within BWI76_RS12025 at 2530.504 kb on + strand, within BWI76_RS12025 at 2530.675 kb on + strand, within BWI76_RS12025 at 2530.727 kb on + strand, within BWI76_RS12025 at 2530.735 kb on - strand, within BWI76_RS12025 at 2531.006 kb on - strand, within BWI76_RS12025 at 2531.085 kb on - strand at 2531.104 kb on + strand at 2531.143 kb on + strand at 2531.149 kb on + strand at 2531.151 kb on - strand at 2531.152 kb on - strand at 2531.226 kb on + strand, within BWI76_RS12030 at 2531.234 kb on - strand at 2531.313 kb on - strand, within BWI76_RS12030 at 2531.423 kb on - strand at 2531.461 kb on + strand, within BWI76_RS12035 at 2531.461 kb on + strand, within BWI76_RS12035 at 2531.461 kb on + strand, within BWI76_RS12035 at 2531.469 kb on - strand, within BWI76_RS12035 at 2531.469 kb on - strand, within BWI76_RS12035 at 2531.469 kb on - strand, within BWI76_RS12035 at 2531.708 kb on + strand, within BWI76_RS12040 at 2531.983 kb on + strand at 2532.114 kb on - strand, within BWI76_RS12045
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL remove 2,529,985 + -0.7 2,530,010 - -0.7 2,530,010 - +0.9 2,530,010 - -0.5 2,530,125 - BWI76_RS12025 0.13 -0.2 2,530,170 + BWI76_RS12025 0.16 -1.3 2,530,269 - BWI76_RS12025 0.24 +0.5 2,530,458 - -1.2 2,530,504 + BWI76_RS12025 0.43 -0.9 2,530,504 + BWI76_RS12025 0.43 -0.1 2,530,504 + BWI76_RS12025 0.43 -1.2 2,530,675 + BWI76_RS12025 0.57 -0.3 2,530,727 + BWI76_RS12025 0.62 -1.5 2,530,735 - BWI76_RS12025 0.62 -0.6 2,531,006 - BWI76_RS12025 0.84 +0.0 2,531,085 - -0.3 2,531,104 + -0.3 2,531,143 + +0.4 2,531,149 + -0.3 2,531,151 - -0.9 2,531,152 - +0.2 2,531,226 + BWI76_RS12030 0.42 +0.3 2,531,234 - -0.4 2,531,313 - BWI76_RS12030 0.69 +1.2 2,531,423 - +0.7 2,531,461 + BWI76_RS12035 0.17 +0.0 2,531,461 + BWI76_RS12035 0.17 -0.3 2,531,461 + BWI76_RS12035 0.17 -0.2 2,531,469 - BWI76_RS12035 0.21 +0.8 2,531,469 - BWI76_RS12035 0.21 -0.5 2,531,469 - BWI76_RS12035 0.21 -0.3 2,531,708 + BWI76_RS12040 0.14 -1.0 2,531,983 + -0.3 2,532,114 - BWI76_RS12045 0.20 -0.9
Or see this region's nucleotide sequence