Experiment: LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01345 and BWI76_RS01350 are separated by 54 nucleotides BWI76_RS01350 and BWI76_RS01355 are separated by 44 nucleotides BWI76_RS01355 and BWI76_RS01360 are separated by 126 nucleotides
BWI76_RS01345: BWI76_RS01345 - 3-octaprenyl-4-hydroxybenzoate decarboxylase, at 274,756 to 276,225
_RS01345
BWI76_RS01350: BWI76_RS01350 - NAD(P)H-flavin reductase, at 276,280 to 276,981
_RS01350
BWI76_RS01355: BWI76_RS01355 - hypothetical protein, at 277,026 to 277,337
_RS01355
BWI76_RS01360: BWI76_RS01360 - acetyl-CoA C-acyltransferase FadA, at 277,464 to 278,627
_RS01360
Position (kb) 276
277 Strain fitness (log2 ratio) -2
-1
0
1 at 276.235 kb on + strand at 276.235 kb on + strand at 276.262 kb on + strand at 276.308 kb on + strand at 276.740 kb on + strand, within BWI76_RS01350 at 277.128 kb on - strand, within BWI76_RS01355 at 277.128 kb on - strand, within BWI76_RS01355 at 277.174 kb on + strand, within BWI76_RS01355 at 277.174 kb on + strand, within BWI76_RS01355 at 277.182 kb on - strand, within BWI76_RS01355 at 277.213 kb on + strand, within BWI76_RS01355 at 277.221 kb on - strand, within BWI76_RS01355 at 277.252 kb on + strand, within BWI76_RS01355 at 277.277 kb on - strand at 277.306 kb on - strand at 277.344 kb on + strand at 277.426 kb on - strand at 277.458 kb on - strand at 277.470 kb on - strand at 277.497 kb on + strand at 277.545 kb on - strand at 277.545 kb on - strand at 277.562 kb on + strand at 277.602 kb on + strand, within BWI76_RS01360 at 277.602 kb on + strand, within BWI76_RS01360 at 277.652 kb on - strand, within BWI76_RS01360 at 277.814 kb on - strand, within BWI76_RS01360 at 277.907 kb on + strand, within BWI76_RS01360 at 277.933 kb on + strand, within BWI76_RS01360
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL remove 276,235 + +0.8 276,235 + +0.0 276,262 + +0.6 276,308 + -0.3 276,740 + BWI76_RS01350 0.66 -1.3 277,128 - BWI76_RS01355 0.33 -1.9 277,128 - BWI76_RS01355 0.33 +1.2 277,174 + BWI76_RS01355 0.47 +1.6 277,174 + BWI76_RS01355 0.47 +0.4 277,182 - BWI76_RS01355 0.50 +0.6 277,213 + BWI76_RS01355 0.60 +0.2 277,221 - BWI76_RS01355 0.62 -0.2 277,252 + BWI76_RS01355 0.72 +0.4 277,277 - +0.1 277,306 - +1.0 277,344 + -0.9 277,426 - +0.3 277,458 - +0.1 277,470 - -0.5 277,497 + +1.0 277,545 - +1.4 277,545 - +0.8 277,562 + +0.3 277,602 + BWI76_RS01360 0.12 -0.2 277,602 + BWI76_RS01360 0.12 -1.7 277,652 - BWI76_RS01360 0.16 +0.4 277,814 - BWI76_RS01360 0.30 -0.2 277,907 + BWI76_RS01360 0.38 +0.1 277,933 + BWI76_RS01360 0.40 -0.0
Or see this region's nucleotide sequence