Experiment: LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS26500 and BWI76_RS26505 overlap by 1 nucleotides BWI76_RS26505 and BWI76_RS26510 overlap by 4 nucleotides BWI76_RS26510 and BWI76_RS26515 overlap by 1 nucleotides BWI76_RS26515 and BWI76_RS26520 overlap by 1 nucleotides
BWI76_RS26500: BWI76_RS26500 - nickel ABC transporter, nickel/metallophore periplasmic binding protein, at 5,484,975 to 5,486,543
_RS26500
BWI76_RS26505: BWI76_RS26505 - nickel ABC transporter permease subunit NikB, at 5,486,543 to 5,487,487
_RS26505
BWI76_RS26510: BWI76_RS26510 - nickel ABC transporter permease subunit NikC, at 5,487,484 to 5,488,317
_RS26510
BWI76_RS26515: BWI76_RS26515 - nickel import ATP-binding protein NikD, at 5,488,317 to 5,489,078
_RS26515
BWI76_RS26520: BWI76_RS26520 - nickel import ATP-binding protein NikE, at 5,489,078 to 5,489,848
_RS26520
Position (kb) 5487
5488
5489 Strain fitness (log2 ratio) -2
-1
0
1 at 5486.493 kb on - strand at 5486.522 kb on + strand at 5486.583 kb on - strand at 5487.167 kb on + strand, within BWI76_RS26505 at 5487.213 kb on + strand, within BWI76_RS26505 at 5487.226 kb on - strand, within BWI76_RS26505 at 5487.305 kb on + strand, within BWI76_RS26505 at 5487.386 kb on - strand, within BWI76_RS26505 at 5487.448 kb on + strand at 5487.663 kb on - strand, within BWI76_RS26510 at 5487.821 kb on + strand, within BWI76_RS26510 at 5487.855 kb on + strand, within BWI76_RS26510 at 5487.880 kb on + strand, within BWI76_RS26510 at 5487.883 kb on - strand, within BWI76_RS26510 at 5487.974 kb on + strand, within BWI76_RS26510 at 5488.014 kb on - strand, within BWI76_RS26510 at 5488.040 kb on + strand, within BWI76_RS26510 at 5488.194 kb on - strand, within BWI76_RS26510 at 5488.222 kb on - strand, within BWI76_RS26510 at 5488.246 kb on + strand at 5488.355 kb on + strand at 5488.588 kb on + strand, within BWI76_RS26515 at 5488.596 kb on - strand, within BWI76_RS26515 at 5488.796 kb on + strand, within BWI76_RS26515 at 5488.972 kb on + strand, within BWI76_RS26515 at 5488.980 kb on - strand, within BWI76_RS26515 at 5489.080 kb on - strand at 5489.089 kb on - strand at 5489.140 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL remove 5,486,493 - -0.8 5,486,522 + +0.2 5,486,583 - -0.1 5,487,167 + BWI76_RS26505 0.66 +0.6 5,487,213 + BWI76_RS26505 0.71 +1.2 5,487,226 - BWI76_RS26505 0.72 +1.5 5,487,305 + BWI76_RS26505 0.81 -0.2 5,487,386 - BWI76_RS26505 0.89 +0.7 5,487,448 + -2.0 5,487,663 - BWI76_RS26510 0.21 +0.7 5,487,821 + BWI76_RS26510 0.40 -0.0 5,487,855 + BWI76_RS26510 0.44 +0.1 5,487,880 + BWI76_RS26510 0.47 -0.0 5,487,883 - BWI76_RS26510 0.48 +1.1 5,487,974 + BWI76_RS26510 0.59 +1.0 5,488,014 - BWI76_RS26510 0.64 -1.1 5,488,040 + BWI76_RS26510 0.67 -0.1 5,488,194 - BWI76_RS26510 0.85 +1.4 5,488,222 - BWI76_RS26510 0.88 -1.1 5,488,246 + -0.1 5,488,355 + -0.5 5,488,588 + BWI76_RS26515 0.36 -0.1 5,488,596 - BWI76_RS26515 0.37 +0.7 5,488,796 + BWI76_RS26515 0.63 -0.4 5,488,972 + BWI76_RS26515 0.86 +0.3 5,488,980 - BWI76_RS26515 0.87 -1.9 5,489,080 - -1.4 5,489,089 - +0.2 5,489,140 + +0.7
Or see this region's nucleotide sequence