Experiment: LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS26495 and BWI76_RS26500 are separated by 374 nucleotides BWI76_RS26500 and BWI76_RS26505 overlap by 1 nucleotides BWI76_RS26505 and BWI76_RS26510 overlap by 4 nucleotides
BWI76_RS26495: BWI76_RS26495 - LysR family transcriptional regulator, at 5,483,740 to 5,484,600
_RS26495
BWI76_RS26500: BWI76_RS26500 - nickel ABC transporter, nickel/metallophore periplasmic binding protein, at 5,484,975 to 5,486,543
_RS26500
BWI76_RS26505: BWI76_RS26505 - nickel ABC transporter permease subunit NikB, at 5,486,543 to 5,487,487
_RS26505
BWI76_RS26510: BWI76_RS26510 - nickel ABC transporter permease subunit NikC, at 5,487,484 to 5,488,317
_RS26510
Position (kb) 5484
5485
5486
5487 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 5483.993 kb on + strand, within BWI76_RS26495 at 5484.001 kb on - strand, within BWI76_RS26495 at 5484.083 kb on + strand, within BWI76_RS26495 at 5484.417 kb on + strand, within BWI76_RS26495 at 5484.585 kb on + strand at 5484.585 kb on + strand at 5484.616 kb on - strand at 5484.616 kb on - strand at 5484.716 kb on + strand at 5485.097 kb on + strand at 5485.151 kb on + strand, within BWI76_RS26500 at 5485.225 kb on + strand, within BWI76_RS26500 at 5485.225 kb on + strand, within BWI76_RS26500 at 5485.225 kb on + strand, within BWI76_RS26500 at 5485.313 kb on - strand, within BWI76_RS26500 at 5485.480 kb on - strand, within BWI76_RS26500 at 5485.638 kb on - strand, within BWI76_RS26500 at 5485.643 kb on - strand, within BWI76_RS26500 at 5485.711 kb on - strand, within BWI76_RS26500 at 5485.796 kb on - strand, within BWI76_RS26500 at 5485.836 kb on + strand, within BWI76_RS26500 at 5485.839 kb on - strand, within BWI76_RS26500 at 5485.886 kb on - strand, within BWI76_RS26500 at 5486.002 kb on + strand, within BWI76_RS26500 at 5486.303 kb on + strand, within BWI76_RS26500 at 5486.464 kb on + strand at 5486.472 kb on - strand at 5486.493 kb on - strand at 5486.522 kb on + strand at 5486.583 kb on - strand at 5487.167 kb on + strand, within BWI76_RS26505 at 5487.213 kb on + strand, within BWI76_RS26505 at 5487.226 kb on - strand, within BWI76_RS26505 at 5487.305 kb on + strand, within BWI76_RS26505 at 5487.386 kb on - strand, within BWI76_RS26505 at 5487.448 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL remove 5,483,993 + BWI76_RS26495 0.29 +0.6 5,484,001 - BWI76_RS26495 0.30 +1.8 5,484,083 + BWI76_RS26495 0.40 +1.5 5,484,417 + BWI76_RS26495 0.79 +0.3 5,484,585 + +0.2 5,484,585 + -0.2 5,484,616 - +0.1 5,484,616 - -1.1 5,484,716 + +1.2 5,485,097 + +0.3 5,485,151 + BWI76_RS26500 0.11 -0.1 5,485,225 + BWI76_RS26500 0.16 +0.1 5,485,225 + BWI76_RS26500 0.16 -1.8 5,485,225 + BWI76_RS26500 0.16 +0.2 5,485,313 - BWI76_RS26500 0.22 +0.8 5,485,480 - BWI76_RS26500 0.32 +0.1 5,485,638 - BWI76_RS26500 0.42 +0.7 5,485,643 - BWI76_RS26500 0.43 +0.7 5,485,711 - BWI76_RS26500 0.47 -0.8 5,485,796 - BWI76_RS26500 0.52 +2.8 5,485,836 + BWI76_RS26500 0.55 +0.5 5,485,839 - BWI76_RS26500 0.55 +0.3 5,485,886 - BWI76_RS26500 0.58 +0.6 5,486,002 + BWI76_RS26500 0.65 +0.2 5,486,303 + BWI76_RS26500 0.85 +0.9 5,486,464 + +0.4 5,486,472 - +0.4 5,486,493 - -0.8 5,486,522 + +0.2 5,486,583 - -0.1 5,487,167 + BWI76_RS26505 0.66 +0.6 5,487,213 + BWI76_RS26505 0.71 +1.2 5,487,226 - BWI76_RS26505 0.72 +1.5 5,487,305 + BWI76_RS26505 0.81 -0.2 5,487,386 - BWI76_RS26505 0.89 +0.7 5,487,448 + -2.0
Or see this region's nucleotide sequence