Experiment: LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS26330 and BWI76_RS26335 are separated by 13 nucleotides BWI76_RS26335 and BWI76_RS26340 overlap by 4 nucleotides BWI76_RS26340 and BWI76_RS26345 overlap by 4 nucleotides BWI76_RS26345 and BWI76_RS26350 are separated by 60 nucleotides
BWI76_RS26330: BWI76_RS26330 - ABC transporter ATP-binding protein, at 5,450,614 to 5,451,315
_RS26330
BWI76_RS26335: BWI76_RS26335 - ABC transporter ATP-binding protein, at 5,451,329 to 5,452,096
_RS26335
BWI76_RS26340: BWI76_RS26340 - branched-chain amino acid ABC transporter permease, at 5,452,093 to 5,453,373
_RS26340
BWI76_RS26345: BWI76_RS26345 - branched-chain amino acid ABC transporter permease LivH, at 5,453,370 to 5,454,296
_RS26345
BWI76_RS26350: BWI76_RS26350 - branched chain amino acid ABC transporter substrate-binding protein, at 5,454,357 to 5,455,466
_RS26350
Position (kb) 5452
5453
5454 Strain fitness (log2 ratio) -2
-1
0
1 at 5451.133 kb on + strand, within BWI76_RS26330 at 5451.230 kb on + strand, within BWI76_RS26330 at 5451.290 kb on + strand at 5451.407 kb on + strand, within BWI76_RS26335 at 5451.472 kb on + strand, within BWI76_RS26335 at 5451.591 kb on + strand, within BWI76_RS26335 at 5451.738 kb on - strand, within BWI76_RS26335 at 5452.303 kb on - strand, within BWI76_RS26340 at 5452.311 kb on - strand, within BWI76_RS26340 at 5452.353 kb on - strand, within BWI76_RS26340 at 5452.518 kb on - strand, within BWI76_RS26340 at 5452.548 kb on + strand at 5452.574 kb on + strand, within BWI76_RS26340 at 5452.712 kb on - strand, within BWI76_RS26340 at 5452.712 kb on - strand, within BWI76_RS26340 at 5452.844 kb on + strand, within BWI76_RS26340 at 5452.885 kb on - strand, within BWI76_RS26340 at 5452.951 kb on + strand, within BWI76_RS26340 at 5452.992 kb on + strand, within BWI76_RS26340 at 5453.083 kb on - strand, within BWI76_RS26340 at 5453.341 kb on + strand at 5453.394 kb on + strand at 5453.556 kb on - strand, within BWI76_RS26345 at 5453.622 kb on - strand, within BWI76_RS26345 at 5453.889 kb on - strand, within BWI76_RS26345 at 5453.955 kb on + strand, within BWI76_RS26345 at 5453.973 kb on - strand, within BWI76_RS26345 at 5454.161 kb on - strand, within BWI76_RS26345 at 5454.243 kb on - strand at 5454.253 kb on - strand at 5454.292 kb on + strand at 5454.292 kb on + strand at 5454.370 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL remove 5,451,133 + BWI76_RS26330 0.74 +0.9 5,451,230 + BWI76_RS26330 0.88 -1.3 5,451,290 + -0.7 5,451,407 + BWI76_RS26335 0.10 +1.2 5,451,472 + BWI76_RS26335 0.19 +0.4 5,451,591 + BWI76_RS26335 0.34 +0.8 5,451,738 - BWI76_RS26335 0.53 +0.6 5,452,303 - BWI76_RS26340 0.16 +0.2 5,452,311 - BWI76_RS26340 0.17 +0.7 5,452,353 - BWI76_RS26340 0.20 -1.0 5,452,518 - BWI76_RS26340 0.33 -0.3 5,452,548 + -0.3 5,452,574 + BWI76_RS26340 0.38 -0.0 5,452,712 - BWI76_RS26340 0.48 -0.1 5,452,712 - BWI76_RS26340 0.48 -1.1 5,452,844 + BWI76_RS26340 0.59 +0.4 5,452,885 - BWI76_RS26340 0.62 +0.0 5,452,951 + BWI76_RS26340 0.67 -1.6 5,452,992 + BWI76_RS26340 0.70 +1.2 5,453,083 - BWI76_RS26340 0.77 -0.4 5,453,341 + -0.2 5,453,394 + -1.3 5,453,556 - BWI76_RS26345 0.20 +0.6 5,453,622 - BWI76_RS26345 0.27 +0.5 5,453,889 - BWI76_RS26345 0.56 +0.3 5,453,955 + BWI76_RS26345 0.63 +0.1 5,453,973 - BWI76_RS26345 0.65 -0.0 5,454,161 - BWI76_RS26345 0.85 +1.2 5,454,243 - -1.2 5,454,253 - -1.9 5,454,292 + +1.2 5,454,292 + +0.5 5,454,370 + -1.3
Or see this region's nucleotide sequence