Experiment: LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS24745 and BWI76_RS24750 are separated by 2 nucleotides BWI76_RS24750 and BWI76_RS24755 are separated by 150 nucleotides BWI76_RS24755 and BWI76_RS24760 are separated by 46 nucleotides BWI76_RS24760 and BWI76_RS24765 are separated by 2 nucleotides
BWI76_RS24745: BWI76_RS24745 - membrane protein, at 5,161,944 to 5,162,606
_RS24745
BWI76_RS24750: BWI76_RS24750 - membrane protein, at 5,162,609 to 5,162,992
_RS24750
BWI76_RS24755: BWI76_RS24755 - hypothetical protein, at 5,163,143 to 5,163,511
_RS24755
BWI76_RS24760: BWI76_RS24760 - membrane protein, at 5,163,558 to 5,163,863
_RS24760
BWI76_RS24765: BWI76_RS24765 - membrane protein, at 5,163,866 to 5,164,264
_RS24765
Position (kb) 5162
5163 Strain fitness (log2 ratio) -2
-1
0
1 at 5161.621 kb on - strand at 5161.652 kb on - strand at 5161.678 kb on + strand at 5161.688 kb on + strand at 5161.836 kb on - strand at 5161.903 kb on - strand at 5161.926 kb on + strand at 5161.964 kb on + strand at 5162.032 kb on - strand, within BWI76_RS24745 at 5162.100 kb on - strand, within BWI76_RS24745 at 5162.194 kb on + strand, within BWI76_RS24745 at 5162.238 kb on + strand, within BWI76_RS24745 at 5162.451 kb on + strand, within BWI76_RS24745 at 5162.648 kb on + strand, within BWI76_RS24750 at 5162.688 kb on - strand, within BWI76_RS24750 at 5162.743 kb on + strand, within BWI76_RS24750 at 5162.866 kb on - strand, within BWI76_RS24750 at 5162.982 kb on + strand at 5163.059 kb on - strand at 5163.069 kb on + strand at 5163.084 kb on + strand at 5163.105 kb on - strand at 5163.105 kb on - strand at 5163.133 kb on + strand at 5163.419 kb on - strand, within BWI76_RS24755 at 5163.489 kb on + strand at 5163.523 kb on + strand at 5163.917 kb on + strand, within BWI76_RS24765 at 5163.920 kb on - strand, within BWI76_RS24765 at 5163.927 kb on + strand, within BWI76_RS24765
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL remove 5,161,621 - +0.4 5,161,652 - -1.4 5,161,678 + -2.1 5,161,688 + -0.1 5,161,836 - -1.4 5,161,903 - +0.4 5,161,926 + +0.4 5,161,964 + +1.0 5,162,032 - BWI76_RS24745 0.13 +0.2 5,162,100 - BWI76_RS24745 0.24 +1.3 5,162,194 + BWI76_RS24745 0.38 -0.3 5,162,238 + BWI76_RS24745 0.44 +1.2 5,162,451 + BWI76_RS24745 0.76 +1.0 5,162,648 + BWI76_RS24750 0.10 -0.4 5,162,688 - BWI76_RS24750 0.21 -2.5 5,162,743 + BWI76_RS24750 0.35 -1.6 5,162,866 - BWI76_RS24750 0.67 -1.4 5,162,982 + +1.1 5,163,059 - -1.1 5,163,069 + -2.1 5,163,084 + -0.3 5,163,105 - -0.4 5,163,105 - -1.2 5,163,133 + -0.8 5,163,419 - BWI76_RS24755 0.75 +1.4 5,163,489 + -1.1 5,163,523 + -0.2 5,163,917 + BWI76_RS24765 0.13 -1.0 5,163,920 - BWI76_RS24765 0.14 -0.6 5,163,927 + BWI76_RS24765 0.15 -2.2
Or see this region's nucleotide sequence