Experiment: LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS23675 and BWI76_RS23680 are separated by 11 nucleotides BWI76_RS23680 and BWI76_RS23685 are separated by 36 nucleotides BWI76_RS23685 and BWI76_RS23690 are separated by 1 nucleotides BWI76_RS23690 and BWI76_RS23695 are separated by 249 nucleotides
BWI76_RS23675: BWI76_RS23675 - allulose-6-phosphate 3-epimerase, at 4,941,491 to 4,942,180
_RS23675
BWI76_RS23680: BWI76_RS23680 - ribokinase, at 4,942,192 to 4,943,133
_RS23680
BWI76_RS23685: BWI76_RS23685 - MFS transporter, at 4,943,170 to 4,944,504
_RS23685
BWI76_RS23690: BWI76_RS23690 - putative glyoxalase/bleomycin resistance protein/dioxygenase, at 4,944,506 to 4,944,895
_RS23690
BWI76_RS23695: BWI76_RS23695 - deoxyribonyl-CoA synthetase <small>(from data)</small>, at 4,945,145 to 4,946,701
_RS23695
Position (kb) 4943
4944
4945 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4942.239 kb on + strand at 4942.428 kb on - strand, within BWI76_RS23680 at 4942.663 kb on + strand, within BWI76_RS23680 at 4942.717 kb on + strand, within BWI76_RS23680 at 4942.927 kb on - strand, within BWI76_RS23680 at 4942.927 kb on - strand, within BWI76_RS23680 at 4942.996 kb on + strand, within BWI76_RS23680 at 4943.142 kb on - strand at 4943.159 kb on - strand at 4943.252 kb on + strand at 4943.473 kb on + strand, within BWI76_RS23685 at 4943.476 kb on - strand, within BWI76_RS23685 at 4943.476 kb on - strand, within BWI76_RS23685 at 4943.523 kb on + strand, within BWI76_RS23685 at 4943.531 kb on - strand, within BWI76_RS23685 at 4943.531 kb on - strand, within BWI76_RS23685 at 4943.532 kb on - strand, within BWI76_RS23685 at 4943.552 kb on + strand, within BWI76_RS23685 at 4943.619 kb on - strand, within BWI76_RS23685 at 4943.806 kb on + strand, within BWI76_RS23685 at 4944.125 kb on - strand, within BWI76_RS23685 at 4944.176 kb on + strand, within BWI76_RS23685 at 4944.290 kb on + strand, within BWI76_RS23685 at 4944.290 kb on + strand, within BWI76_RS23685 at 4944.298 kb on - strand, within BWI76_RS23685 at 4944.345 kb on - strand, within BWI76_RS23685 at 4944.633 kb on + strand, within BWI76_RS23690 at 4944.682 kb on + strand, within BWI76_RS23690 at 4944.957 kb on + strand at 4944.965 kb on - strand at 4945.069 kb on - strand at 4945.185 kb on + strand at 4945.303 kb on + strand, within BWI76_RS23695 at 4945.323 kb on - strand, within BWI76_RS23695 at 4945.347 kb on - strand, within BWI76_RS23695 at 4945.423 kb on + strand, within BWI76_RS23695 at 4945.424 kb on - strand, within BWI76_RS23695 at 4945.493 kb on + strand, within BWI76_RS23695 at 4945.501 kb on - strand, within BWI76_RS23695
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL remove 4,942,239 + +1.4 4,942,428 - BWI76_RS23680 0.25 -3.3 4,942,663 + BWI76_RS23680 0.50 -0.2 4,942,717 + BWI76_RS23680 0.56 +0.2 4,942,927 - BWI76_RS23680 0.78 -1.7 4,942,927 - BWI76_RS23680 0.78 -0.2 4,942,996 + BWI76_RS23680 0.85 -0.9 4,943,142 - -1.5 4,943,159 - -0.7 4,943,252 + -1.0 4,943,473 + BWI76_RS23685 0.23 +1.5 4,943,476 - BWI76_RS23685 0.23 -2.3 4,943,476 - BWI76_RS23685 0.23 +1.3 4,943,523 + BWI76_RS23685 0.26 +0.6 4,943,531 - BWI76_RS23685 0.27 +0.3 4,943,531 - BWI76_RS23685 0.27 +0.7 4,943,532 - BWI76_RS23685 0.27 +0.5 4,943,552 + BWI76_RS23685 0.29 +0.4 4,943,619 - BWI76_RS23685 0.34 -0.8 4,943,806 + BWI76_RS23685 0.48 +0.9 4,944,125 - BWI76_RS23685 0.72 -0.9 4,944,176 + BWI76_RS23685 0.75 -0.2 4,944,290 + BWI76_RS23685 0.84 +2.4 4,944,290 + BWI76_RS23685 0.84 -0.2 4,944,298 - BWI76_RS23685 0.84 -0.0 4,944,345 - BWI76_RS23685 0.88 +1.1 4,944,633 + BWI76_RS23690 0.33 +0.9 4,944,682 + BWI76_RS23690 0.45 +1.6 4,944,957 + -1.2 4,944,965 - -1.5 4,945,069 - -0.4 4,945,185 + +0.5 4,945,303 + BWI76_RS23695 0.10 +0.6 4,945,323 - BWI76_RS23695 0.11 +0.9 4,945,347 - BWI76_RS23695 0.13 -0.8 4,945,423 + BWI76_RS23695 0.18 -0.9 4,945,424 - BWI76_RS23695 0.18 +0.0 4,945,493 + BWI76_RS23695 0.22 -0.2 4,945,501 - BWI76_RS23695 0.23 +0.7
Or see this region's nucleotide sequence