Experiment: LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS17670 and BWI76_RS17675 overlap by 4 nucleotides BWI76_RS17675 and BWI76_RS17680 overlap by 7 nucleotides BWI76_RS17680 and BWI76_RS17685 are separated by 294 nucleotides
BWI76_RS17670: BWI76_RS17670 - transglutaminase, at 3,670,260 to 3,671,075
_RS17670
BWI76_RS17675: BWI76_RS17675 - hypothetical protein, at 3,671,072 to 3,672,001
_RS17675
BWI76_RS17680: BWI76_RS17680 - hypothetical protein, at 3,671,995 to 3,673,434
_RS17680
BWI76_RS17685: BWI76_RS17685 - beta-glucosidase, at 3,673,729 to 3,676,509
_RS17685
Position (kb)
3671
3672
3673
3674 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3671.105 kb on + strand at 3671.377 kb on - strand, within BWI76_RS17675 at 3671.538 kb on - strand, within BWI76_RS17675 at 3671.694 kb on - strand, within BWI76_RS17675 at 3671.967 kb on + strand at 3672.191 kb on - strand, within BWI76_RS17680 at 3672.268 kb on - strand, within BWI76_RS17680 at 3672.342 kb on + strand, within BWI76_RS17680 at 3672.350 kb on - strand, within BWI76_RS17680 at 3672.429 kb on + strand, within BWI76_RS17680 at 3672.453 kb on + strand, within BWI76_RS17680 at 3672.465 kb on - strand, within BWI76_RS17680 at 3672.657 kb on - strand, within BWI76_RS17680 at 3672.657 kb on - strand, within BWI76_RS17680 at 3672.704 kb on - strand, within BWI76_RS17680 at 3672.745 kb on + strand, within BWI76_RS17680 at 3672.843 kb on + strand, within BWI76_RS17680 at 3672.843 kb on + strand, within BWI76_RS17680 at 3672.848 kb on + strand, within BWI76_RS17680 at 3672.856 kb on - strand, within BWI76_RS17680 at 3673.262 kb on - strand, within BWI76_RS17680 at 3673.508 kb on - strand at 3673.531 kb on - strand at 3673.655 kb on - strand at 3673.679 kb on - strand at 3673.985 kb on - strand at 3674.050 kb on - strand, within BWI76_RS17685 at 3674.050 kb on - strand, within BWI76_RS17685 at 3674.100 kb on + strand, within BWI76_RS17685 at 3674.166 kb on + strand, within BWI76_RS17685 at 3674.217 kb on - strand, within BWI76_RS17685 at 3674.374 kb on + strand, within BWI76_RS17685 at 3674.391 kb on + strand, within BWI76_RS17685
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL remove 3,671,105 + +0.2 3,671,377 - BWI76_RS17675 0.33 +1.5 3,671,538 - BWI76_RS17675 0.50 +1.0 3,671,694 - BWI76_RS17675 0.67 -1.6 3,671,967 + +0.9 3,672,191 - BWI76_RS17680 0.14 +2.0 3,672,268 - BWI76_RS17680 0.19 +1.6 3,672,342 + BWI76_RS17680 0.24 +1.4 3,672,350 - BWI76_RS17680 0.25 -0.7 3,672,429 + BWI76_RS17680 0.30 +0.9 3,672,453 + BWI76_RS17680 0.32 +1.7 3,672,465 - BWI76_RS17680 0.33 +2.1 3,672,657 - BWI76_RS17680 0.46 +0.8 3,672,657 - BWI76_RS17680 0.46 +0.8 3,672,704 - BWI76_RS17680 0.49 -0.0 3,672,745 + BWI76_RS17680 0.52 +0.5 3,672,843 + BWI76_RS17680 0.59 +0.3 3,672,843 + BWI76_RS17680 0.59 -2.6 3,672,848 + BWI76_RS17680 0.59 +0.1 3,672,856 - BWI76_RS17680 0.60 +1.2 3,673,262 - BWI76_RS17680 0.88 -0.1 3,673,508 - -0.4 3,673,531 - -0.7 3,673,655 - +1.0 3,673,679 - +0.8 3,673,985 - -2.0 3,674,050 - BWI76_RS17685 0.12 +0.1 3,674,050 - BWI76_RS17685 0.12 -3.0 3,674,100 + BWI76_RS17685 0.13 +1.4 3,674,166 + BWI76_RS17685 0.16 +0.3 3,674,217 - BWI76_RS17685 0.18 +0.3 3,674,374 + BWI76_RS17685 0.23 -1.9 3,674,391 + BWI76_RS17685 0.24 -0.2
Or see this region's nucleotide sequence