Experiment: LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS17345 and BWI76_RS17350 are separated by 10 nucleotides BWI76_RS17350 and BWI76_RS17355 overlap by 4 nucleotides BWI76_RS17355 and BWI76_RS17360 are separated by 102 nucleotides
BWI76_RS17345: BWI76_RS17345 - nitrate/nitrite two-component system sensor histidine kinase NarX, at 3,594,148 to 3,595,926
_RS17345
BWI76_RS17350: BWI76_RS17350 - DNA-binding response regulator, at 3,595,937 to 3,596,587
_RS17350
BWI76_RS17355: BWI76_RS17355 - YchO family inverse autotransporter domain-containing protein, at 3,596,584 to 3,597,963
_RS17355
BWI76_RS17360: BWI76_RS17360 - nitrate reductase, at 3,598,066 to 3,600,666
_RS17360
Position (kb) 3596
3597
3598 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 3595.701 kb on + strand, within BWI76_RS17345 at 3596.379 kb on + strand, within BWI76_RS17350 at 3596.476 kb on + strand, within BWI76_RS17350 at 3596.924 kb on - strand, within BWI76_RS17355 at 3597.047 kb on - strand, within BWI76_RS17355 at 3597.338 kb on - strand, within BWI76_RS17355 at 3597.382 kb on - strand, within BWI76_RS17355 at 3597.444 kb on - strand, within BWI76_RS17355 at 3597.522 kb on + strand, within BWI76_RS17355 at 3597.537 kb on + strand, within BWI76_RS17355 at 3597.545 kb on - strand, within BWI76_RS17355 at 3597.858 kb on - strand at 3597.879 kb on - strand at 3598.128 kb on - strand at 3598.212 kb on + strand at 3598.911 kb on + strand, within BWI76_RS17360
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL remove 3,595,701 + BWI76_RS17345 0.87 +0.2 3,596,379 + BWI76_RS17350 0.68 -0.7 3,596,476 + BWI76_RS17350 0.83 +0.9 3,596,924 - BWI76_RS17355 0.25 -3.2 3,597,047 - BWI76_RS17355 0.34 +0.0 3,597,338 - BWI76_RS17355 0.55 -1.1 3,597,382 - BWI76_RS17355 0.58 +0.6 3,597,444 - BWI76_RS17355 0.62 -0.5 3,597,522 + BWI76_RS17355 0.68 -0.7 3,597,537 + BWI76_RS17355 0.69 -0.7 3,597,545 - BWI76_RS17355 0.70 -0.9 3,597,858 - -0.4 3,597,879 - +0.2 3,598,128 - -1.0 3,598,212 + -0.5 3,598,911 + BWI76_RS17360 0.32 +0.2
Or see this region's nucleotide sequence