Experiment: LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS17275 and BWI76_RS17280 are separated by 89 nucleotides BWI76_RS17280 and BWI76_RS17285 are separated by 112 nucleotides BWI76_RS17285 and BWI76_RS17290 are separated by 42 nucleotides
BWI76_RS17275: BWI76_RS17275 - two-component system response regulator RssB, at 3,579,844 to 3,580,857
_RS17275
BWI76_RS17280: BWI76_RS17280 - patatin family protein, at 3,580,947 to 3,581,846
_RS17280
BWI76_RS17285: BWI76_RS17285 - hypothetical protein, at 3,581,959 to 3,582,417
_RS17285
BWI76_RS17290: BWI76_RS17290 - formyltetrahydrofolate deformylase, at 3,582,460 to 3,583,302
_RS17290
Position (kb)
3580
3581
3582 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3579.992 kb on + strand, within BWI76_RS17275 at 3579.992 kb on + strand, within BWI76_RS17275 at 3580.000 kb on - strand at 3580.000 kb on - strand, within BWI76_RS17275 at 3580.000 kb on - strand, within BWI76_RS17275 at 3580.022 kb on - strand, within BWI76_RS17275 at 3580.061 kb on - strand, within BWI76_RS17275 at 3580.088 kb on + strand, within BWI76_RS17275 at 3580.096 kb on - strand, within BWI76_RS17275 at 3580.117 kb on - strand, within BWI76_RS17275 at 3580.140 kb on - strand, within BWI76_RS17275 at 3580.142 kb on + strand, within BWI76_RS17275 at 3580.142 kb on + strand, within BWI76_RS17275 at 3580.150 kb on - strand, within BWI76_RS17275 at 3580.150 kb on - strand, within BWI76_RS17275 at 3580.150 kb on - strand, within BWI76_RS17275 at 3580.150 kb on - strand, within BWI76_RS17275 at 3580.184 kb on + strand, within BWI76_RS17275 at 3580.184 kb on + strand, within BWI76_RS17275 at 3580.184 kb on + strand, within BWI76_RS17275 at 3580.184 kb on + strand, within BWI76_RS17275 at 3580.192 kb on - strand, within BWI76_RS17275 at 3580.192 kb on - strand, within BWI76_RS17275 at 3580.192 kb on - strand, within BWI76_RS17275 at 3580.219 kb on - strand, within BWI76_RS17275 at 3580.299 kb on - strand, within BWI76_RS17275 at 3580.301 kb on + strand, within BWI76_RS17275 at 3580.373 kb on + strand, within BWI76_RS17275 at 3580.383 kb on + strand, within BWI76_RS17275 at 3580.400 kb on - strand, within BWI76_RS17275 at 3580.400 kb on - strand, within BWI76_RS17275 at 3580.569 kb on + strand, within BWI76_RS17275 at 3580.786 kb on + strand at 3580.794 kb on - strand at 3580.794 kb on - strand at 3580.868 kb on - strand at 3580.868 kb on - strand at 3580.898 kb on + strand at 3580.904 kb on + strand at 3580.949 kb on + strand at 3580.949 kb on + strand at 3580.957 kb on - strand at 3580.977 kb on - strand at 3581.020 kb on + strand at 3581.114 kb on - strand, within BWI76_RS17280 at 3581.423 kb on + strand, within BWI76_RS17280 at 3581.423 kb on + strand, within BWI76_RS17280 at 3581.581 kb on - strand, within BWI76_RS17280 at 3581.997 kb on - strand at 3582.047 kb on + strand, within BWI76_RS17285 at 3582.105 kb on + strand, within BWI76_RS17285 at 3582.151 kb on + strand, within BWI76_RS17285 at 3582.182 kb on + strand, within BWI76_RS17285 at 3582.221 kb on + strand, within BWI76_RS17285 at 3582.270 kb on - strand, within BWI76_RS17285 at 3582.321 kb on + strand, within BWI76_RS17285 at 3582.321 kb on + strand, within BWI76_RS17285 at 3582.482 kb on - strand at 3582.500 kb on - strand at 3582.663 kb on - strand, within BWI76_RS17290 at 3582.663 kb on - strand at 3582.706 kb on - strand, within BWI76_RS17290 at 3582.763 kb on - strand, within BWI76_RS17290
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL remove 3,579,992 + BWI76_RS17275 0.15 -2.6 3,579,992 + BWI76_RS17275 0.15 -0.1 3,580,000 - -0.7 3,580,000 - BWI76_RS17275 0.15 +0.0 3,580,000 - BWI76_RS17275 0.15 -2.2 3,580,022 - BWI76_RS17275 0.18 -0.2 3,580,061 - BWI76_RS17275 0.21 +0.3 3,580,088 + BWI76_RS17275 0.24 +1.4 3,580,096 - BWI76_RS17275 0.25 +0.8 3,580,117 - BWI76_RS17275 0.27 +1.9 3,580,140 - BWI76_RS17275 0.29 +1.2 3,580,142 + BWI76_RS17275 0.29 +0.2 3,580,142 + BWI76_RS17275 0.29 +0.8 3,580,150 - BWI76_RS17275 0.30 +0.2 3,580,150 - BWI76_RS17275 0.30 -0.5 3,580,150 - BWI76_RS17275 0.30 -0.1 3,580,150 - BWI76_RS17275 0.30 -0.4 3,580,184 + BWI76_RS17275 0.34 +0.6 3,580,184 + BWI76_RS17275 0.34 -0.1 3,580,184 + BWI76_RS17275 0.34 -1.7 3,580,184 + BWI76_RS17275 0.34 +0.6 3,580,192 - BWI76_RS17275 0.34 -0.2 3,580,192 - BWI76_RS17275 0.34 +1.0 3,580,192 - BWI76_RS17275 0.34 +0.9 3,580,219 - BWI76_RS17275 0.37 +0.1 3,580,299 - BWI76_RS17275 0.45 -1.1 3,580,301 + BWI76_RS17275 0.45 +0.5 3,580,373 + BWI76_RS17275 0.52 +0.4 3,580,383 + BWI76_RS17275 0.53 +1.6 3,580,400 - BWI76_RS17275 0.55 +0.7 3,580,400 - BWI76_RS17275 0.55 -0.1 3,580,569 + BWI76_RS17275 0.71 -0.2 3,580,786 + +0.1 3,580,794 - -0.1 3,580,794 - -0.9 3,580,868 - +0.3 3,580,868 - +0.5 3,580,898 + +0.6 3,580,904 + -0.9 3,580,949 + +0.6 3,580,949 + -1.3 3,580,957 - -0.0 3,580,977 - +1.0 3,581,020 + -0.4 3,581,114 - BWI76_RS17280 0.19 -3.2 3,581,423 + BWI76_RS17280 0.53 +0.3 3,581,423 + BWI76_RS17280 0.53 -1.0 3,581,581 - BWI76_RS17280 0.70 -0.2 3,581,997 - -0.6 3,582,047 + BWI76_RS17285 0.19 -0.3 3,582,105 + BWI76_RS17285 0.32 +1.2 3,582,151 + BWI76_RS17285 0.42 +1.2 3,582,182 + BWI76_RS17285 0.49 -0.9 3,582,221 + BWI76_RS17285 0.57 +0.2 3,582,270 - BWI76_RS17285 0.68 -1.7 3,582,321 + BWI76_RS17285 0.79 -1.1 3,582,321 + BWI76_RS17285 0.79 +0.3 3,582,482 - +0.2 3,582,500 - +0.7 3,582,663 - BWI76_RS17290 0.24 -1.7 3,582,663 - -0.1 3,582,706 - BWI76_RS17290 0.29 -0.9 3,582,763 - BWI76_RS17290 0.36 +0.4
Or see this region's nucleotide sequence