Experiment: LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16805 and BWI76_RS16810 are separated by 95 nucleotides BWI76_RS16810 and BWI76_RS16815 overlap by 4 nucleotides BWI76_RS16815 and BWI76_RS16820 are separated by 16 nucleotides
BWI76_RS16805: BWI76_RS16805 - basic amino acid ABC transporter substrate-binding protein, at 3,484,185 to 3,484,952
_RS16805
BWI76_RS16810: BWI76_RS16810 - RpiR family transcriptional regulator, at 3,485,048 to 3,485,947
_RS16810
BWI76_RS16815: BWI76_RS16815 - periplasmic binding protein, at 3,485,944 to 3,486,891
_RS16815
BWI76_RS16820: BWI76_RS16820 - iron ABC transporter, at 3,486,908 to 3,487,960
_RS16820
Position (kb) 3485
3486
3487 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 3484.949 kb on - strand at 3485.168 kb on + strand, within BWI76_RS16810 at 3485.322 kb on - strand, within BWI76_RS16810 at 3485.639 kb on - strand, within BWI76_RS16810 at 3485.694 kb on - strand, within BWI76_RS16810 at 3486.017 kb on + strand at 3486.017 kb on + strand at 3486.032 kb on - strand at 3486.033 kb on - strand at 3486.588 kb on - strand, within BWI76_RS16815 at 3486.654 kb on + strand, within BWI76_RS16815 at 3487.049 kb on + strand, within BWI76_RS16820 at 3487.075 kb on + strand, within BWI76_RS16820 at 3487.353 kb on - strand, within BWI76_RS16820 at 3487.378 kb on + strand, within BWI76_RS16820 at 3487.656 kb on - strand, within BWI76_RS16820 at 3487.656 kb on - strand, within BWI76_RS16820 at 3487.738 kb on + strand, within BWI76_RS16820
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL remove 3,484,949 - +0.4 3,485,168 + BWI76_RS16810 0.13 +0.5 3,485,322 - BWI76_RS16810 0.30 -0.8 3,485,639 - BWI76_RS16810 0.66 +0.1 3,485,694 - BWI76_RS16810 0.72 -0.9 3,486,017 + +1.2 3,486,017 + -2.8 3,486,032 - +0.0 3,486,033 - +2.7 3,486,588 - BWI76_RS16815 0.68 -1.9 3,486,654 + BWI76_RS16815 0.75 -1.7 3,487,049 + BWI76_RS16820 0.13 +0.8 3,487,075 + BWI76_RS16820 0.16 -1.2 3,487,353 - BWI76_RS16820 0.42 +0.4 3,487,378 + BWI76_RS16820 0.45 +1.0 3,487,656 - BWI76_RS16820 0.71 -0.7 3,487,656 - BWI76_RS16820 0.71 +0.7 3,487,738 + BWI76_RS16820 0.79 +0.6
Or see this region's nucleotide sequence