Experiment: LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16740 and BWI76_RS16745 are separated by 106 nucleotides BWI76_RS16745 and BWI76_RS16750 are separated by 10 nucleotides BWI76_RS16750 and BWI76_RS16755 are separated by 23 nucleotides BWI76_RS16755 and BWI76_RS16760 are separated by 57 nucleotides
BWI76_RS16740: BWI76_RS16740 - transcriptional regulator, at 3,467,493 to 3,468,365
_RS16740
BWI76_RS16745: BWI76_RS16745 - alpha-acetolactate decarboxylase, at 3,468,472 to 3,469,251
_RS16745
BWI76_RS16750: BWI76_RS16750 - acetolactate synthase, at 3,469,262 to 3,470,941
_RS16750
BWI76_RS16755: BWI76_RS16755 - acetoin reductase, at 3,470,965 to 3,471,735
_RS16755
BWI76_RS16760: BWI76_RS16760 - lipase, at 3,471,793 to 3,472,863
_RS16760
Position (kb) 3469
3470
3471 Strain fitness (log2 ratio) -2
-1
0
1
2 at 3468.518 kb on - strand at 3468.524 kb on - strand at 3468.549 kb on - strand at 3468.559 kb on + strand, within BWI76_RS16745 at 3468.576 kb on - strand, within BWI76_RS16745 at 3468.576 kb on - strand, within BWI76_RS16745 at 3468.585 kb on + strand, within BWI76_RS16745 at 3468.585 kb on + strand, within BWI76_RS16745 at 3468.800 kb on + strand, within BWI76_RS16745 at 3469.030 kb on - strand, within BWI76_RS16745 at 3469.079 kb on + strand, within BWI76_RS16745 at 3469.307 kb on - strand at 3469.523 kb on - strand, within BWI76_RS16750 at 3469.701 kb on - strand, within BWI76_RS16750 at 3469.998 kb on - strand, within BWI76_RS16750 at 3470.542 kb on - strand, within BWI76_RS16750 at 3470.625 kb on - strand, within BWI76_RS16750 at 3470.627 kb on + strand, within BWI76_RS16750 at 3470.662 kb on + strand, within BWI76_RS16750 at 3470.746 kb on + strand, within BWI76_RS16750 at 3470.925 kb on - strand at 3471.142 kb on - strand, within BWI76_RS16755 at 3471.229 kb on - strand, within BWI76_RS16755 at 3471.754 kb on - strand at 3471.859 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL remove 3,468,518 - -0.1 3,468,524 - +0.9 3,468,549 - +0.6 3,468,559 + BWI76_RS16745 0.11 +1.3 3,468,576 - BWI76_RS16745 0.13 +0.3 3,468,576 - BWI76_RS16745 0.13 +0.3 3,468,585 + BWI76_RS16745 0.14 +2.3 3,468,585 + BWI76_RS16745 0.14 +0.5 3,468,800 + BWI76_RS16745 0.42 +0.8 3,469,030 - BWI76_RS16745 0.72 -0.2 3,469,079 + BWI76_RS16745 0.78 +0.7 3,469,307 - +0.5 3,469,523 - BWI76_RS16750 0.16 +0.2 3,469,701 - BWI76_RS16750 0.26 +0.8 3,469,998 - BWI76_RS16750 0.44 +1.5 3,470,542 - BWI76_RS16750 0.76 +0.8 3,470,625 - BWI76_RS16750 0.81 +0.6 3,470,627 + BWI76_RS16750 0.81 +0.4 3,470,662 + BWI76_RS16750 0.83 -1.9 3,470,746 + BWI76_RS16750 0.88 -0.1 3,470,925 - +0.8 3,471,142 - BWI76_RS16755 0.23 +0.8 3,471,229 - BWI76_RS16755 0.34 +1.1 3,471,754 - +0.0 3,471,859 - -0.2
Or see this region's nucleotide sequence