Experiment: LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16000 and BWI76_RS16005 overlap by 14 nucleotides BWI76_RS16005 and BWI76_RS16010 are separated by 30 nucleotides BWI76_RS16010 and BWI76_RS16015 are separated by 171 nucleotides BWI76_RS16015 and BWI76_RS16020 are separated by 437 nucleotides
BWI76_RS16000: BWI76_RS16000 - multidrug transporter subunit MdtI, at 3,319,441 to 3,319,770
_RS16000
BWI76_RS16005: BWI76_RS16005 - multidrug transporter subunit MdtJ, at 3,319,757 to 3,320,119
_RS16005
BWI76_RS16010: BWI76_RS16010 - hypothetical protein, at 3,320,150 to 3,320,473
_RS16010
BWI76_RS16015: BWI76_RS16015 - pheromone autoinducer 2 transporter, at 3,320,645 to 3,321,679
_RS16015
BWI76_RS16020: BWI76_RS16020 - malonate decarboxylase subunit alpha, at 3,322,117 to 3,323,772
_RS16020
Position (kb)
3320
3321
3322 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3319.739 kb on - strand at 3319.739 kb on - strand at 3319.745 kb on + strand at 3319.754 kb on + strand at 3319.758 kb on - strand at 3319.762 kb on - strand at 3319.830 kb on + strand, within BWI76_RS16005 at 3319.839 kb on + strand at 3319.852 kb on - strand, within BWI76_RS16005 at 3319.886 kb on + strand, within BWI76_RS16005 at 3319.888 kb on + strand, within BWI76_RS16005 at 3319.888 kb on + strand, within BWI76_RS16005 at 3319.896 kb on - strand, within BWI76_RS16005 at 3319.912 kb on + strand, within BWI76_RS16005 at 3319.912 kb on + strand, within BWI76_RS16005 at 3319.920 kb on - strand, within BWI76_RS16005 at 3319.920 kb on - strand, within BWI76_RS16005 at 3319.920 kb on - strand, within BWI76_RS16005 at 3319.920 kb on - strand, within BWI76_RS16005 at 3319.943 kb on + strand, within BWI76_RS16005 at 3319.944 kb on - strand, within BWI76_RS16005 at 3319.944 kb on - strand, within BWI76_RS16005 at 3319.944 kb on - strand, within BWI76_RS16005 at 3319.945 kb on - strand, within BWI76_RS16005 at 3319.951 kb on - strand, within BWI76_RS16005 at 3319.951 kb on - strand, within BWI76_RS16005 at 3319.952 kb on - strand, within BWI76_RS16005 at 3319.952 kb on - strand, within BWI76_RS16005 at 3320.123 kb on + strand at 3320.131 kb on - strand at 3320.131 kb on - strand at 3320.156 kb on - strand at 3320.214 kb on - strand, within BWI76_RS16010 at 3320.223 kb on - strand, within BWI76_RS16010 at 3320.223 kb on - strand, within BWI76_RS16010 at 3320.227 kb on + strand, within BWI76_RS16010 at 3320.229 kb on + strand, within BWI76_RS16010 at 3320.242 kb on - strand, within BWI76_RS16010 at 3320.245 kb on - strand, within BWI76_RS16010 at 3320.258 kb on - strand, within BWI76_RS16010 at 3320.283 kb on + strand, within BWI76_RS16010 at 3320.285 kb on - strand, within BWI76_RS16010 at 3320.301 kb on - strand, within BWI76_RS16010 at 3320.405 kb on - strand, within BWI76_RS16010 at 3320.411 kb on + strand, within BWI76_RS16010 at 3320.419 kb on - strand, within BWI76_RS16010 at 3320.425 kb on + strand, within BWI76_RS16010 at 3320.432 kb on - strand, within BWI76_RS16010 at 3320.460 kb on - strand at 3320.594 kb on + strand at 3320.594 kb on + strand at 3320.594 kb on + strand at 3320.594 kb on + strand at 3320.594 kb on + strand at 3320.595 kb on + strand at 3320.599 kb on + strand at 3320.602 kb on - strand at 3320.602 kb on - strand at 3320.602 kb on - strand at 3320.602 kb on - strand at 3320.602 kb on - strand at 3320.602 kb on - strand at 3320.602 kb on - strand at 3320.603 kb on - strand at 3320.613 kb on - strand at 3320.638 kb on + strand at 3320.648 kb on + strand at 3320.704 kb on + strand at 3320.713 kb on + strand at 3320.728 kb on - strand at 3321.032 kb on - strand, within BWI76_RS16015 at 3321.088 kb on + strand, within BWI76_RS16015 at 3321.150 kb on + strand, within BWI76_RS16015 at 3321.477 kb on + strand, within BWI76_RS16015 at 3321.756 kb on - strand at 3321.872 kb on + strand at 3321.891 kb on - strand at 3322.068 kb on + strand at 3322.445 kb on + strand, within BWI76_RS16020 at 3322.509 kb on - strand, within BWI76_RS16020 at 3322.596 kb on + strand, within BWI76_RS16020 at 3322.604 kb on - strand, within BWI76_RS16020
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL remove 3,319,739 - +0.2 3,319,739 - -0.2 3,319,745 + +0.8 3,319,754 + +0.3 3,319,758 - -0.3 3,319,762 - -1.5 3,319,830 + BWI76_RS16005 0.20 -0.3 3,319,839 + -0.9 3,319,852 - BWI76_RS16005 0.26 -0.4 3,319,886 + BWI76_RS16005 0.36 +0.6 3,319,888 + BWI76_RS16005 0.36 -0.3 3,319,888 + BWI76_RS16005 0.36 +1.1 3,319,896 - BWI76_RS16005 0.38 -0.4 3,319,912 + BWI76_RS16005 0.43 +0.1 3,319,912 + BWI76_RS16005 0.43 -2.4 3,319,920 - BWI76_RS16005 0.45 -0.1 3,319,920 - BWI76_RS16005 0.45 +1.4 3,319,920 - BWI76_RS16005 0.45 -0.3 3,319,920 - BWI76_RS16005 0.45 +0.2 3,319,943 + BWI76_RS16005 0.51 +0.7 3,319,944 - BWI76_RS16005 0.52 -0.3 3,319,944 - BWI76_RS16005 0.52 -1.3 3,319,944 - BWI76_RS16005 0.52 +0.1 3,319,945 - BWI76_RS16005 0.52 +0.5 3,319,951 - BWI76_RS16005 0.53 +0.5 3,319,951 - BWI76_RS16005 0.53 -4.2 3,319,952 - BWI76_RS16005 0.54 -0.4 3,319,952 - BWI76_RS16005 0.54 -1.3 3,320,123 + -0.8 3,320,131 - +0.3 3,320,131 - +0.3 3,320,156 - -0.7 3,320,214 - BWI76_RS16010 0.20 +0.5 3,320,223 - BWI76_RS16010 0.23 -0.0 3,320,223 - BWI76_RS16010 0.23 -0.8 3,320,227 + BWI76_RS16010 0.24 +0.4 3,320,229 + BWI76_RS16010 0.24 +0.6 3,320,242 - BWI76_RS16010 0.28 -2.8 3,320,245 - BWI76_RS16010 0.29 -2.3 3,320,258 - BWI76_RS16010 0.33 +1.0 3,320,283 + BWI76_RS16010 0.41 -0.3 3,320,285 - BWI76_RS16010 0.42 +0.7 3,320,301 - BWI76_RS16010 0.47 -2.2 3,320,405 - BWI76_RS16010 0.79 -2.4 3,320,411 + BWI76_RS16010 0.81 -1.7 3,320,419 - BWI76_RS16010 0.83 -1.9 3,320,425 + BWI76_RS16010 0.85 +1.4 3,320,432 - BWI76_RS16010 0.87 +0.1 3,320,460 - +0.6 3,320,594 + -1.0 3,320,594 + +0.7 3,320,594 + +2.0 3,320,594 + +0.9 3,320,594 + -0.5 3,320,595 + +1.2 3,320,599 + +1.1 3,320,602 - +0.3 3,320,602 - +1.1 3,320,602 - +0.4 3,320,602 - +0.5 3,320,602 - -0.4 3,320,602 - -0.9 3,320,602 - +0.0 3,320,603 - +2.0 3,320,613 - +1.3 3,320,638 + +1.6 3,320,648 + +0.1 3,320,704 + +1.8 3,320,713 + +0.7 3,320,728 - +0.4 3,321,032 - BWI76_RS16015 0.37 +1.4 3,321,088 + BWI76_RS16015 0.43 +1.1 3,321,150 + BWI76_RS16015 0.49 +1.9 3,321,477 + BWI76_RS16015 0.80 +0.5 3,321,756 - -1.7 3,321,872 + -3.0 3,321,891 - +0.1 3,322,068 + +0.1 3,322,445 + BWI76_RS16020 0.20 -0.5 3,322,509 - BWI76_RS16020 0.24 +1.3 3,322,596 + BWI76_RS16020 0.29 -0.1 3,322,604 - BWI76_RS16020 0.29 -2.7
Or see this region's nucleotide sequence