Experiment: LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS15345 and BWI76_RS15350 are separated by 149 nucleotides BWI76_RS15350 and BWI76_RS15355 are separated by 67 nucleotides BWI76_RS15355 and BWI76_RS15360 are separated by 423 nucleotides BWI76_RS15360 and BWI76_RS15365 are separated by 88 nucleotides
BWI76_RS15345: BWI76_RS15345 - 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase, at 3,214,024 to 3,215,127
_RS15345
BWI76_RS15350: BWI76_RS15350 - hypothetical protein, at 3,215,277 to 3,215,669
_RS15350
BWI76_RS15355: BWI76_RS15355 - transcriptional regulator FtrA, at 3,215,737 to 3,216,747
_RS15355
BWI76_RS15360: BWI76_RS15360 - hypothetical protein, at 3,217,171 to 3,217,506
_RS15360
BWI76_RS15365: BWI76_RS15365 - hypothetical protein, at 3,217,595 to 3,217,927
_RS15365
Position (kb)
3215
3216
3217 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3214.844 kb on - strand, within BWI76_RS15345 at 3215.104 kb on + strand at 3215.295 kb on + strand at 3215.297 kb on - strand at 3215.303 kb on - strand at 3215.354 kb on - strand, within BWI76_RS15350 at 3216.042 kb on + strand, within BWI76_RS15355 at 3216.052 kb on + strand, within BWI76_RS15355 at 3216.220 kb on + strand, within BWI76_RS15355 at 3216.228 kb on - strand, within BWI76_RS15355 at 3216.510 kb on - strand, within BWI76_RS15355 at 3216.603 kb on - strand, within BWI76_RS15355 at 3216.604 kb on - strand, within BWI76_RS15355 at 3216.609 kb on - strand at 3216.617 kb on + strand at 3216.617 kb on + strand, within BWI76_RS15355 at 3216.625 kb on - strand, within BWI76_RS15355 at 3216.625 kb on - strand, within BWI76_RS15355 at 3216.625 kb on - strand, within BWI76_RS15355 at 3216.654 kb on + strand at 3216.669 kb on - strand at 3216.727 kb on - strand at 3216.805 kb on + strand at 3216.806 kb on + strand at 3216.811 kb on + strand at 3216.825 kb on + strand at 3216.825 kb on + strand at 3216.825 kb on + strand at 3216.825 kb on + strand at 3216.825 kb on + strand at 3216.825 kb on + strand at 3216.833 kb on - strand at 3216.833 kb on - strand at 3216.833 kb on - strand at 3216.833 kb on - strand at 3216.833 kb on - strand at 3216.833 kb on - strand at 3216.834 kb on + strand at 3216.843 kb on - strand at 3216.844 kb on + strand at 3216.844 kb on + strand at 3216.844 kb on + strand at 3216.844 kb on + strand at 3216.844 kb on + strand at 3216.852 kb on - strand at 3216.875 kb on + strand at 3216.875 kb on + strand at 3216.985 kb on + strand at 3216.998 kb on + strand at 3217.026 kb on + strand at 3217.033 kb on - strand at 3217.034 kb on - strand at 3217.034 kb on - strand at 3217.080 kb on - strand at 3217.106 kb on - strand at 3217.123 kb on - strand at 3217.226 kb on + strand, within BWI76_RS15360 at 3217.229 kb on - strand, within BWI76_RS15360 at 3217.234 kb on - strand, within BWI76_RS15360 at 3217.293 kb on + strand, within BWI76_RS15360 at 3217.293 kb on + strand, within BWI76_RS15360 at 3217.463 kb on + strand, within BWI76_RS15360
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL remove 3,214,844 - BWI76_RS15345 0.74 +0.9 3,215,104 + -0.8 3,215,295 + -1.0 3,215,297 - +1.2 3,215,303 - +0.1 3,215,354 - BWI76_RS15350 0.20 -1.9 3,216,042 + BWI76_RS15355 0.30 -1.5 3,216,052 + BWI76_RS15355 0.31 +0.2 3,216,220 + BWI76_RS15355 0.48 -0.0 3,216,228 - BWI76_RS15355 0.49 -0.1 3,216,510 - BWI76_RS15355 0.76 -0.9 3,216,603 - BWI76_RS15355 0.86 -0.3 3,216,604 - BWI76_RS15355 0.86 +0.3 3,216,609 - +1.6 3,216,617 + +1.0 3,216,617 + BWI76_RS15355 0.87 -1.3 3,216,625 - BWI76_RS15355 0.88 +0.6 3,216,625 - BWI76_RS15355 0.88 -1.8 3,216,625 - BWI76_RS15355 0.88 +0.6 3,216,654 + -2.2 3,216,669 - +0.6 3,216,727 - +0.8 3,216,805 + +1.0 3,216,806 + -1.3 3,216,811 + +0.5 3,216,825 + -1.6 3,216,825 + +1.3 3,216,825 + -0.9 3,216,825 + -0.4 3,216,825 + +0.1 3,216,825 + -1.0 3,216,833 - +1.3 3,216,833 - +1.0 3,216,833 - +2.0 3,216,833 - +2.7 3,216,833 - +2.3 3,216,833 - -0.6 3,216,834 + +1.6 3,216,843 - -0.6 3,216,844 + -0.4 3,216,844 + -0.9 3,216,844 + +0.3 3,216,844 + -0.5 3,216,844 + +0.2 3,216,852 - -1.0 3,216,875 + +1.0 3,216,875 + +0.3 3,216,985 + +0.2 3,216,998 + +0.7 3,217,026 + +0.5 3,217,033 - -0.3 3,217,034 - -1.3 3,217,034 - +0.1 3,217,080 - -0.0 3,217,106 - -0.3 3,217,123 - -2.6 3,217,226 + BWI76_RS15360 0.16 +1.3 3,217,229 - BWI76_RS15360 0.17 -1.0 3,217,234 - BWI76_RS15360 0.19 +3.7 3,217,293 + BWI76_RS15360 0.36 +0.7 3,217,293 + BWI76_RS15360 0.36 -1.6 3,217,463 + BWI76_RS15360 0.87 -0.7
Or see this region's nucleotide sequence