Experiment: LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS15055 and BWI76_RS15060 are separated by 429 nucleotides BWI76_RS15060 and BWI76_RS15065 are separated by 114 nucleotides BWI76_RS15065 and BWI76_RS15070 are separated by 36 nucleotides
BWI76_RS15055: BWI76_RS15055 - lysine transporter LysE, at 3,151,275 to 3,151,895
_RS15055
BWI76_RS15060: BWI76_RS15060 - MarR family transcriptional regulator, at 3,152,325 to 3,152,822
_RS15060
BWI76_RS15065: BWI76_RS15065 - hypothetical protein, at 3,152,937 to 3,153,329
_RS15065
BWI76_RS15070: BWI76_RS15070 - non-ribosomal peptide synthetase, at 3,153,366 to 3,157,736
_RS15070
Position (kb)
3152
3153 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3151.390 kb on + strand, within BWI76_RS15055 at 3151.462 kb on + strand, within BWI76_RS15055 at 3151.577 kb on - strand, within BWI76_RS15055 at 3151.596 kb on + strand, within BWI76_RS15055 at 3151.604 kb on - strand, within BWI76_RS15055 at 3151.627 kb on - strand, within BWI76_RS15055 at 3151.662 kb on - strand, within BWI76_RS15055 at 3151.671 kb on - strand, within BWI76_RS15055 at 3151.673 kb on + strand, within BWI76_RS15055 at 3151.680 kb on + strand, within BWI76_RS15055 at 3151.680 kb on + strand, within BWI76_RS15055 at 3151.681 kb on - strand, within BWI76_RS15055 at 3151.682 kb on - strand, within BWI76_RS15055 at 3151.682 kb on - strand, within BWI76_RS15055 at 3151.688 kb on - strand, within BWI76_RS15055 at 3151.688 kb on - strand, within BWI76_RS15055 at 3151.701 kb on - strand, within BWI76_RS15055 at 3151.708 kb on - strand, within BWI76_RS15055 at 3151.708 kb on - strand, within BWI76_RS15055 at 3151.712 kb on + strand, within BWI76_RS15055 at 3151.727 kb on - strand, within BWI76_RS15055 at 3151.746 kb on + strand at 3151.761 kb on - strand, within BWI76_RS15055 at 3151.822 kb on + strand, within BWI76_RS15055 at 3151.843 kb on - strand at 3151.848 kb on - strand at 3151.871 kb on - strand at 3151.951 kb on + strand at 3151.951 kb on + strand at 3151.951 kb on + strand at 3151.951 kb on + strand at 3151.951 kb on + strand at 3151.959 kb on - strand at 3151.959 kb on - strand at 3151.959 kb on - strand at 3151.990 kb on - strand at 3152.129 kb on + strand at 3152.438 kb on - strand, within BWI76_RS15060 at 3152.442 kb on - strand, within BWI76_RS15060 at 3152.457 kb on - strand, within BWI76_RS15060 at 3152.495 kb on - strand, within BWI76_RS15060 at 3152.695 kb on - strand, within BWI76_RS15060 at 3152.729 kb on - strand, within BWI76_RS15060 at 3153.179 kb on - strand, within BWI76_RS15065 at 3153.293 kb on - strand at 3153.293 kb on - strand at 3153.414 kb on - strand at 3153.428 kb on + strand at 3153.428 kb on + strand at 3153.436 kb on - strand at 3153.436 kb on - strand at 3153.436 kb on - strand at 3153.436 kb on - strand at 3153.436 kb on - strand at 3153.436 kb on - strand at 3153.452 kb on + strand at 3153.452 kb on + strand at 3153.452 kb on + strand at 3153.469 kb on - strand at 3153.540 kb on + strand at 3153.544 kb on - strand at 3153.548 kb on - strand at 3153.555 kb on + strand at 3153.555 kb on + strand at 3153.555 kb on + strand at 3153.562 kb on + strand at 3153.568 kb on - strand at 3153.572 kb on + strand at 3153.580 kb on - strand at 3153.778 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL remove 3,151,390 + BWI76_RS15055 0.19 +0.6 3,151,462 + BWI76_RS15055 0.30 +0.1 3,151,577 - BWI76_RS15055 0.49 -0.3 3,151,596 + BWI76_RS15055 0.52 +0.7 3,151,604 - BWI76_RS15055 0.53 -3.5 3,151,627 - BWI76_RS15055 0.57 +0.6 3,151,662 - BWI76_RS15055 0.62 -0.5 3,151,671 - BWI76_RS15055 0.64 -0.1 3,151,673 + BWI76_RS15055 0.64 -0.7 3,151,680 + BWI76_RS15055 0.65 -1.7 3,151,680 + BWI76_RS15055 0.65 -0.4 3,151,681 - BWI76_RS15055 0.65 +0.3 3,151,682 - BWI76_RS15055 0.66 +0.5 3,151,682 - BWI76_RS15055 0.66 -0.1 3,151,688 - BWI76_RS15055 0.67 +0.5 3,151,688 - BWI76_RS15055 0.67 +0.3 3,151,701 - BWI76_RS15055 0.69 +0.3 3,151,708 - BWI76_RS15055 0.70 -0.4 3,151,708 - BWI76_RS15055 0.70 +0.3 3,151,712 + BWI76_RS15055 0.70 -0.7 3,151,727 - BWI76_RS15055 0.73 -0.1 3,151,746 + +1.0 3,151,761 - BWI76_RS15055 0.78 -2.0 3,151,822 + BWI76_RS15055 0.88 -1.0 3,151,843 - +0.6 3,151,848 - -0.1 3,151,871 - +1.1 3,151,951 + +0.1 3,151,951 + +1.3 3,151,951 + -0.6 3,151,951 + +1.0 3,151,951 + -3.1 3,151,959 - +0.3 3,151,959 - -0.3 3,151,959 - -0.4 3,151,990 - -1.7 3,152,129 + +0.1 3,152,438 - BWI76_RS15060 0.23 +3.6 3,152,442 - BWI76_RS15060 0.23 -1.7 3,152,457 - BWI76_RS15060 0.27 -1.6 3,152,495 - BWI76_RS15060 0.34 -1.7 3,152,695 - BWI76_RS15060 0.74 -0.9 3,152,729 - BWI76_RS15060 0.81 -3.3 3,153,179 - BWI76_RS15065 0.62 -0.6 3,153,293 - +1.0 3,153,293 - -1.7 3,153,414 - +0.1 3,153,428 + -2.2 3,153,428 + +1.0 3,153,436 - +0.9 3,153,436 - -0.3 3,153,436 - +1.0 3,153,436 - +1.2 3,153,436 - +0.5 3,153,436 - -2.2 3,153,452 + +3.0 3,153,452 + +1.0 3,153,452 + +0.5 3,153,469 - +2.8 3,153,540 + +1.2 3,153,544 - -0.0 3,153,548 - -0.6 3,153,555 + +1.0 3,153,555 + -0.0 3,153,555 + -2.5 3,153,562 + +1.6 3,153,568 - +1.3 3,153,572 + +1.0 3,153,580 - +0.8 3,153,778 - +0.2
Or see this region's nucleotide sequence