Experiment: LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS13545 and BWI76_RS13550 are separated by 9 nucleotides BWI76_RS13550 and BWI76_RS13555 overlap by 17 nucleotides BWI76_RS13555 and BWI76_RS13560 overlap by 4 nucleotides BWI76_RS13560 and BWI76_RS13565 are separated by 65 nucleotides
BWI76_RS13545: BWI76_RS13545 - N-acetyltransferase, at 2,824,481 to 2,824,990
_RS13545
BWI76_RS13550: BWI76_RS13550 - polar amino acid ABC transporter inner membrane subunit, at 2,825,000 to 2,825,926
_RS13550
BWI76_RS13555: BWI76_RS13555 - ABC transporter, at 2,825,910 to 2,826,689
_RS13555
BWI76_RS13560: BWI76_RS13560 - amino acid ABC transporter substrate-binding subunit, at 2,826,686 to 2,827,579
_RS13560
BWI76_RS13565: BWI76_RS13565 - MFS transporter, at 2,827,645 to 2,828,955
_RS13565
Position (kb) 2825
2826
2827 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 2824.910 kb on + strand, within BWI76_RS13545 at 2825.210 kb on + strand, within BWI76_RS13550 at 2825.514 kb on - strand, within BWI76_RS13550 at 2825.514 kb on - strand, within BWI76_RS13550 at 2825.538 kb on - strand, within BWI76_RS13550 at 2825.652 kb on - strand, within BWI76_RS13550 at 2825.689 kb on + strand, within BWI76_RS13550 at 2825.697 kb on - strand, within BWI76_RS13550 at 2825.716 kb on - strand, within BWI76_RS13550 at 2825.791 kb on + strand, within BWI76_RS13550 at 2825.902 kb on - strand at 2825.906 kb on + strand at 2826.466 kb on - strand, within BWI76_RS13555 at 2826.987 kb on + strand, within BWI76_RS13560
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL remove 2,824,910 + BWI76_RS13545 0.84 -4.1 2,825,210 + BWI76_RS13550 0.23 +0.2 2,825,514 - BWI76_RS13550 0.55 -0.7 2,825,514 - BWI76_RS13550 0.55 -0.4 2,825,538 - BWI76_RS13550 0.58 +1.4 2,825,652 - BWI76_RS13550 0.70 +0.1 2,825,689 + BWI76_RS13550 0.74 +2.5 2,825,697 - BWI76_RS13550 0.75 +0.2 2,825,716 - BWI76_RS13550 0.77 -0.0 2,825,791 + BWI76_RS13550 0.85 +0.5 2,825,902 - +0.8 2,825,906 + -0.6 2,826,466 - BWI76_RS13555 0.71 +0.4 2,826,987 + BWI76_RS13560 0.34 -2.7
Or see this region's nucleotide sequence