Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS11175

Experiment: LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS11165 and BWI76_RS11170 are separated by 36 nucleotidesBWI76_RS11170 and BWI76_RS11175 are separated by 233 nucleotidesBWI76_RS11175 and BWI76_RS11180 are separated by 225 nucleotidesBWI76_RS11180 and BWI76_RS11185 are separated by 56 nucleotides BWI76_RS11165: BWI76_RS11165 - beta-N-acetylhexosaminidase, at 2,352,837 to 2,353,859 _RS11165 BWI76_RS11170: BWI76_RS11170 - hypothetical protein, at 2,353,896 to 2,354,438 _RS11170 BWI76_RS11175: BWI76_RS11175 - NADH dehydrogenase, at 2,354,672 to 2,355,976 _RS11175 BWI76_RS11180: BWI76_RS11180 - hypothetical protein, at 2,356,202 to 2,356,741 _RS11180 BWI76_RS11185: BWI76_RS11185 - ligand-gated channel protein, at 2,356,798 to 2,358,975 _RS11185 Position (kb) 2354 2355 2356Strain fitness (log2 ratio) -2 -1 0 1at 2353.799 kb on - strandat 2353.821 kb on - strandat 2354.214 kb on - strand, within BWI76_RS11170at 2354.721 kb on + strandat 2356.272 kb on + strand, within BWI76_RS11180at 2356.313 kb on + strand, within BWI76_RS11180at 2356.561 kb on + strand, within BWI76_RS11180at 2356.707 kb on + strandat 2356.867 kb on + strandat 2356.880 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
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2,353,799 - -2.6
2,353,821 - -0.1
2,354,214 - BWI76_RS11170 0.59 +0.7
2,354,721 + +0.1
2,356,272 + BWI76_RS11180 0.13 +0.2
2,356,313 + BWI76_RS11180 0.21 +0.3
2,356,561 + BWI76_RS11180 0.66 -0.1
2,356,707 + -0.1
2,356,867 + -0.6
2,356,880 - +0.9

Or see this region's nucleotide sequence