Experiment: LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10915 and BWI76_RS10920 are separated by 88 nucleotides BWI76_RS10920 and BWI76_RS10925 are separated by 36 nucleotides BWI76_RS10925 and BWI76_RS10930 are separated by 252 nucleotides
BWI76_RS10915: BWI76_RS10915 - O-acetyl-ADP-ribose deacetylase, at 2,304,960 to 2,305,514
_RS10915
BWI76_RS10920: BWI76_RS10920 - putative TetR family transcriptional regulator, at 2,305,603 to 2,306,151
_RS10920
BWI76_RS10925: BWI76_RS10925 - glucans biosynthesis protein C, at 2,306,188 to 2,307,348
_RS10925
BWI76_RS10930: BWI76_RS10930 - glucan biosynthesis protein G, at 2,307,601 to 2,309,154
_RS10930
Position (kb)
2306
2307
2308 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2305.350 kb on - strand, within BWI76_RS10915 at 2305.417 kb on - strand, within BWI76_RS10915 at 2305.515 kb on - strand at 2305.631 kb on - strand at 2305.631 kb on - strand at 2305.644 kb on + strand at 2305.682 kb on + strand, within BWI76_RS10920 at 2305.694 kb on + strand, within BWI76_RS10920 at 2305.809 kb on - strand, within BWI76_RS10920 at 2305.932 kb on - strand, within BWI76_RS10920 at 2306.156 kb on + strand at 2306.176 kb on - strand at 2306.325 kb on + strand, within BWI76_RS10925 at 2306.366 kb on + strand, within BWI76_RS10925 at 2306.366 kb on + strand, within BWI76_RS10925 at 2306.366 kb on + strand, within BWI76_RS10925 at 2306.374 kb on - strand, within BWI76_RS10925 at 2306.374 kb on - strand, within BWI76_RS10925 at 2306.374 kb on - strand, within BWI76_RS10925 at 2306.374 kb on - strand, within BWI76_RS10925 at 2306.391 kb on + strand, within BWI76_RS10925 at 2306.431 kb on + strand, within BWI76_RS10925 at 2306.666 kb on - strand, within BWI76_RS10925 at 2306.736 kb on - strand, within BWI76_RS10925 at 2306.982 kb on + strand, within BWI76_RS10925 at 2307.040 kb on + strand, within BWI76_RS10925 at 2307.048 kb on - strand, within BWI76_RS10925 at 2307.076 kb on + strand, within BWI76_RS10925 at 2307.229 kb on + strand, within BWI76_RS10925 at 2307.229 kb on + strand at 2307.229 kb on + strand, within BWI76_RS10925 at 2307.440 kb on - strand at 2307.440 kb on - strand at 2307.462 kb on + strand at 2307.470 kb on - strand at 2307.483 kb on + strand at 2307.483 kb on + strand at 2307.491 kb on - strand at 2307.491 kb on - strand at 2307.492 kb on - strand at 2307.635 kb on + strand at 2307.635 kb on + strand at 2307.646 kb on + strand at 2307.654 kb on - strand at 2307.689 kb on + strand at 2307.734 kb on + strand at 2307.742 kb on - strand at 2307.759 kb on + strand, within BWI76_RS10930 at 2307.767 kb on - strand, within BWI76_RS10930 at 2307.767 kb on - strand, within BWI76_RS10930 at 2307.782 kb on + strand, within BWI76_RS10930 at 2307.978 kb on + strand, within BWI76_RS10930 at 2307.991 kb on + strand, within BWI76_RS10930 at 2307.991 kb on + strand, within BWI76_RS10930 at 2307.991 kb on + strand, within BWI76_RS10930 at 2307.991 kb on + strand, within BWI76_RS10930 at 2307.997 kb on - strand, within BWI76_RS10930 at 2307.999 kb on - strand, within BWI76_RS10930 at 2308.325 kb on - strand, within BWI76_RS10930
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL remove 2,305,350 - BWI76_RS10915 0.70 +0.7 2,305,417 - BWI76_RS10915 0.82 -0.2 2,305,515 - +0.3 2,305,631 - +1.2 2,305,631 - +0.7 2,305,644 + -0.8 2,305,682 + BWI76_RS10920 0.14 -2.8 2,305,694 + BWI76_RS10920 0.17 -0.4 2,305,809 - BWI76_RS10920 0.38 -1.8 2,305,932 - BWI76_RS10920 0.60 -0.6 2,306,156 + +0.2 2,306,176 - -0.1 2,306,325 + BWI76_RS10925 0.12 -1.4 2,306,366 + BWI76_RS10925 0.15 -1.5 2,306,366 + BWI76_RS10925 0.15 -1.7 2,306,366 + BWI76_RS10925 0.15 +0.6 2,306,374 - BWI76_RS10925 0.16 +0.1 2,306,374 - BWI76_RS10925 0.16 +0.2 2,306,374 - BWI76_RS10925 0.16 -3.8 2,306,374 - BWI76_RS10925 0.16 +0.1 2,306,391 + BWI76_RS10925 0.17 +0.0 2,306,431 + BWI76_RS10925 0.21 -2.2 2,306,666 - BWI76_RS10925 0.41 -0.6 2,306,736 - BWI76_RS10925 0.47 -0.9 2,306,982 + BWI76_RS10925 0.68 -2.4 2,307,040 + BWI76_RS10925 0.73 +1.5 2,307,048 - BWI76_RS10925 0.74 -0.9 2,307,076 + BWI76_RS10925 0.76 +0.2 2,307,229 + BWI76_RS10925 0.90 +0.6 2,307,229 + -0.1 2,307,229 + BWI76_RS10925 0.90 -0.2 2,307,440 - +1.0 2,307,440 - +0.3 2,307,462 + +0.6 2,307,470 - +0.0 2,307,483 + -0.3 2,307,483 + +2.3 2,307,491 - -1.1 2,307,491 - -1.3 2,307,492 - +0.0 2,307,635 + -2.0 2,307,635 + -2.7 2,307,646 + +0.9 2,307,654 - -1.9 2,307,689 + -0.1 2,307,734 + -4.3 2,307,742 - +0.1 2,307,759 + BWI76_RS10930 0.10 -2.2 2,307,767 - BWI76_RS10930 0.11 -1.6 2,307,767 - BWI76_RS10930 0.11 -2.5 2,307,782 + BWI76_RS10930 0.12 -2.6 2,307,978 + BWI76_RS10930 0.24 -0.5 2,307,991 + BWI76_RS10930 0.25 -4.1 2,307,991 + BWI76_RS10930 0.25 -0.6 2,307,991 + BWI76_RS10930 0.25 -1.6 2,307,991 + BWI76_RS10930 0.25 -1.1 2,307,997 - BWI76_RS10930 0.25 -0.1 2,307,999 - BWI76_RS10930 0.26 -0.9 2,308,325 - BWI76_RS10930 0.47 -0.1
Or see this region's nucleotide sequence