Experiment: LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS07685 and BWI76_RS07690 are separated by 53 nucleotides BWI76_RS07690 and BWI76_RS07695 are separated by 67 nucleotides BWI76_RS07695 and BWI76_RS07700 overlap by 8 nucleotides BWI76_RS07700 and BWI76_RS07705 are separated by 64 nucleotides
BWI76_RS07685: BWI76_RS07685 - transketolase, at 1,628,162 to 1,629,097
_RS07685
BWI76_RS07690: BWI76_RS07690 - enterochelin synthetase component D, at 1,629,151 to 1,629,774
_RS07690
BWI76_RS07695: BWI76_RS07695 - TonB-dependent siderophore receptor, at 1,629,842 to 1,632,091
_RS07695
BWI76_RS07700: BWI76_RS07700 - hypothetical protein, at 1,632,084 to 1,632,297
_RS07700
BWI76_RS07705: BWI76_RS07705 - enterochelin esterase, at 1,632,362 to 1,633,561
_RS07705
Position (kb)
1629
1630
1631
1632
1633 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1629.163 kb on + strand at 1629.281 kb on - strand, within BWI76_RS07690 at 1629.496 kb on + strand, within BWI76_RS07690 at 1629.507 kb on + strand, within BWI76_RS07690 at 1629.515 kb on - strand, within BWI76_RS07690 at 1629.542 kb on + strand, within BWI76_RS07690 at 1629.685 kb on - strand, within BWI76_RS07690 at 1630.091 kb on + strand, within BWI76_RS07695 at 1630.099 kb on - strand, within BWI76_RS07695 at 1630.143 kb on + strand, within BWI76_RS07695 at 1630.186 kb on + strand at 1630.189 kb on - strand, within BWI76_RS07695 at 1630.391 kb on + strand, within BWI76_RS07695 at 1630.574 kb on + strand, within BWI76_RS07695 at 1630.574 kb on + strand, within BWI76_RS07695 at 1630.582 kb on - strand, within BWI76_RS07695 at 1630.582 kb on - strand, within BWI76_RS07695 at 1630.611 kb on + strand, within BWI76_RS07695 at 1630.712 kb on - strand, within BWI76_RS07695 at 1630.849 kb on - strand, within BWI76_RS07695 at 1630.927 kb on + strand, within BWI76_RS07695 at 1631.014 kb on + strand, within BWI76_RS07695 at 1631.539 kb on + strand, within BWI76_RS07695 at 1631.758 kb on + strand, within BWI76_RS07695 at 1631.842 kb on + strand, within BWI76_RS07695 at 1631.850 kb on - strand, within BWI76_RS07695 at 1632.194 kb on + strand, within BWI76_RS07700 at 1632.372 kb on - strand at 1632.427 kb on - strand at 1632.635 kb on - strand, within BWI76_RS07705 at 1632.875 kb on - strand, within BWI76_RS07705 at 1632.971 kb on + strand, within BWI76_RS07705
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL remove 1,629,163 + -0.1 1,629,281 - BWI76_RS07690 0.21 +0.5 1,629,496 + BWI76_RS07690 0.55 +0.4 1,629,507 + BWI76_RS07690 0.57 -0.0 1,629,515 - BWI76_RS07690 0.58 -0.5 1,629,542 + BWI76_RS07690 0.63 +0.1 1,629,685 - BWI76_RS07690 0.86 +0.4 1,630,091 + BWI76_RS07695 0.11 +0.4 1,630,099 - BWI76_RS07695 0.11 -1.3 1,630,143 + BWI76_RS07695 0.13 +1.9 1,630,186 + -0.5 1,630,189 - BWI76_RS07695 0.15 +1.4 1,630,391 + BWI76_RS07695 0.24 -0.3 1,630,574 + BWI76_RS07695 0.33 -2.8 1,630,574 + BWI76_RS07695 0.33 +3.0 1,630,582 - BWI76_RS07695 0.33 +0.1 1,630,582 - BWI76_RS07695 0.33 -1.3 1,630,611 + BWI76_RS07695 0.34 +0.9 1,630,712 - BWI76_RS07695 0.39 +0.4 1,630,849 - BWI76_RS07695 0.45 +1.5 1,630,927 + BWI76_RS07695 0.48 -0.2 1,631,014 + BWI76_RS07695 0.52 -2.3 1,631,539 + BWI76_RS07695 0.75 +0.4 1,631,758 + BWI76_RS07695 0.85 -0.3 1,631,842 + BWI76_RS07695 0.89 +0.7 1,631,850 - BWI76_RS07695 0.89 -0.5 1,632,194 + BWI76_RS07700 0.51 +1.1 1,632,372 - +0.2 1,632,427 - +1.1 1,632,635 - BWI76_RS07705 0.23 -0.4 1,632,875 - BWI76_RS07705 0.43 -1.7 1,632,971 + BWI76_RS07705 0.51 +1.6
Or see this region's nucleotide sequence