Experiment: LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS07520 and BWI76_RS07525 are separated by 18 nucleotides BWI76_RS07525 and BWI76_RS07530 are separated by 2 nucleotides BWI76_RS07530 and BWI76_RS07535 are separated by 3 nucleotides
BWI76_RS07520: BWI76_RS07520 - ABC transporter substrate-binding protein, at 1,591,167 to 1,592,831
_RS07520
BWI76_RS07525: BWI76_RS07525 - ABC transporter permease, at 1,592,850 to 1,593,803
_RS07525
BWI76_RS07530: BWI76_RS07530 - binding-protein-dependent transport system inner membrane protein, at 1,593,806 to 1,594,699
_RS07530
BWI76_RS07535: BWI76_RS07535 - dipeptide/oligopeptide/nickel ABC transporter ATP-binding protein, at 1,594,703 to 1,595,746
_RS07535
Position (kb) 1592
1593
1594 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 1592.148 kb on + strand, within BWI76_RS07520 at 1592.194 kb on - strand, within BWI76_RS07520 at 1592.396 kb on - strand, within BWI76_RS07520 at 1592.458 kb on - strand, within BWI76_RS07520 at 1592.573 kb on - strand, within BWI76_RS07520 at 1592.935 kb on - strand at 1593.006 kb on - strand, within BWI76_RS07525 at 1593.134 kb on + strand, within BWI76_RS07525 at 1593.180 kb on + strand, within BWI76_RS07525 at 1593.234 kb on - strand, within BWI76_RS07525 at 1593.281 kb on + strand, within BWI76_RS07525 at 1593.463 kb on + strand, within BWI76_RS07525 at 1593.775 kb on + strand at 1593.808 kb on + strand at 1594.222 kb on + strand, within BWI76_RS07530 at 1594.316 kb on - strand, within BWI76_RS07530 at 1594.397 kb on + strand, within BWI76_RS07530 at 1594.521 kb on - strand, within BWI76_RS07530 at 1594.597 kb on + strand, within BWI76_RS07530
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL remove 1,592,148 + BWI76_RS07520 0.59 +0.0 1,592,194 - BWI76_RS07520 0.62 -1.9 1,592,396 - BWI76_RS07520 0.74 +0.2 1,592,458 - BWI76_RS07520 0.78 +0.0 1,592,573 - BWI76_RS07520 0.84 -0.1 1,592,935 - -1.6 1,593,006 - BWI76_RS07525 0.16 -1.0 1,593,134 + BWI76_RS07525 0.30 -0.8 1,593,180 + BWI76_RS07525 0.35 -0.7 1,593,234 - BWI76_RS07525 0.40 -3.8 1,593,281 + BWI76_RS07525 0.45 +1.6 1,593,463 + BWI76_RS07525 0.64 -1.0 1,593,775 + -3.3 1,593,808 + +0.6 1,594,222 + BWI76_RS07530 0.47 -0.6 1,594,316 - BWI76_RS07530 0.57 +0.0 1,594,397 + BWI76_RS07530 0.66 +0.8 1,594,521 - BWI76_RS07530 0.80 +0.1 1,594,597 + BWI76_RS07530 0.88 -0.1
Or see this region's nucleotide sequence