Experiment: LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04815 and BWI76_RS04820 overlap by 1 nucleotides BWI76_RS04820 and BWI76_RS04825 are separated by 347 nucleotides BWI76_RS04825 and BWI76_RS04830 are separated by 29 nucleotides
BWI76_RS04815: BWI76_RS04815 - cell division protein ZapD, at 1,006,026 to 1,006,769
_RS04815
BWI76_RS04820: BWI76_RS04820 - dephospho-CoA kinase, at 1,006,769 to 1,007,389
_RS04820
BWI76_RS04825: BWI76_RS04825 - guanosine monophosphate reductase, at 1,007,737 to 1,008,780
_RS04825
BWI76_RS04830: BWI76_RS04830 - type IV pilin biogenesis protein, at 1,008,810 to 1,010,015
_RS04830
Position (kb) 1007
1008
1009 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 1007.616 kb on - strand at 1007.661 kb on + strand at 1007.665 kb on + strand at 1007.692 kb on + strand at 1007.852 kb on + strand, within BWI76_RS04825 at 1007.862 kb on + strand, within BWI76_RS04825 at 1007.862 kb on + strand, within BWI76_RS04825 at 1007.903 kb on - strand, within BWI76_RS04825 at 1007.967 kb on + strand at 1007.983 kb on - strand, within BWI76_RS04825 at 1008.029 kb on + strand, within BWI76_RS04825 at 1008.037 kb on - strand, within BWI76_RS04825 at 1008.331 kb on - strand, within BWI76_RS04825 at 1008.531 kb on + strand, within BWI76_RS04825 at 1008.539 kb on - strand, within BWI76_RS04825 at 1008.578 kb on + strand, within BWI76_RS04825 at 1008.583 kb on + strand, within BWI76_RS04825 at 1008.878 kb on - strand at 1009.028 kb on + strand, within BWI76_RS04830 at 1009.028 kb on + strand, within BWI76_RS04830 at 1009.267 kb on + strand, within BWI76_RS04830 at 1009.418 kb on + strand, within BWI76_RS04830 at 1009.480 kb on + strand, within BWI76_RS04830 at 1009.486 kb on + strand, within BWI76_RS04830 at 1009.562 kb on - strand, within BWI76_RS04830 at 1009.712 kb on + strand, within BWI76_RS04830 at 1009.714 kb on + strand, within BWI76_RS04830 at 1009.777 kb on + strand, within BWI76_RS04830
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL remove 1,007,616 - +0.3 1,007,661 + -1.0 1,007,665 + -0.1 1,007,692 + +0.0 1,007,852 + BWI76_RS04825 0.11 +0.1 1,007,862 + BWI76_RS04825 0.12 +2.1 1,007,862 + BWI76_RS04825 0.12 -0.7 1,007,903 - BWI76_RS04825 0.16 +1.7 1,007,967 + +1.1 1,007,983 - BWI76_RS04825 0.24 -0.4 1,008,029 + BWI76_RS04825 0.28 -1.3 1,008,037 - BWI76_RS04825 0.29 -2.7 1,008,331 - BWI76_RS04825 0.57 +0.5 1,008,531 + BWI76_RS04825 0.76 -0.7 1,008,539 - BWI76_RS04825 0.77 +0.9 1,008,578 + BWI76_RS04825 0.81 +0.2 1,008,583 + BWI76_RS04825 0.81 -0.4 1,008,878 - -0.1 1,009,028 + BWI76_RS04830 0.18 +1.4 1,009,028 + BWI76_RS04830 0.18 +0.0 1,009,267 + BWI76_RS04830 0.38 +0.2 1,009,418 + BWI76_RS04830 0.50 +2.5 1,009,480 + BWI76_RS04830 0.56 +0.1 1,009,486 + BWI76_RS04830 0.56 +3.1 1,009,562 - BWI76_RS04830 0.62 +1.5 1,009,712 + BWI76_RS04830 0.75 -0.0 1,009,714 + BWI76_RS04830 0.75 +0.0 1,009,777 + BWI76_RS04830 0.80 -0.8
Or see this region's nucleotide sequence