Experiment: LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04805 and BWI76_RS04810 are separated by 52 nucleotides BWI76_RS04810 and BWI76_RS04815 are separated by 9 nucleotides BWI76_RS04815 and BWI76_RS04820 overlap by 1 nucleotides BWI76_RS04820 and BWI76_RS04825 are separated by 347 nucleotides
BWI76_RS04805: BWI76_RS04805 - 8-oxo-dGTP diphosphatase, at 1,005,362 to 1,005,769
_RS04805
BWI76_RS04810: BWI76_RS04810 - DNA gyrase inhibitor YacG, at 1,005,822 to 1,006,016
_RS04810
BWI76_RS04815: BWI76_RS04815 - cell division protein ZapD, at 1,006,026 to 1,006,769
_RS04815
BWI76_RS04820: BWI76_RS04820 - dephospho-CoA kinase, at 1,006,769 to 1,007,389
_RS04820
BWI76_RS04825: BWI76_RS04825 - guanosine monophosphate reductase, at 1,007,737 to 1,008,780
_RS04825
Position (kb) 1006
1007
1008 Strain fitness (log2 ratio) -2
-1
0
1
2 at 1006.038 kb on - strand at 1006.523 kb on + strand, within BWI76_RS04815 at 1006.583 kb on + strand, within BWI76_RS04815 at 1006.613 kb on - strand, within BWI76_RS04815 at 1006.623 kb on - strand, within BWI76_RS04815 at 1007.616 kb on - strand at 1007.661 kb on + strand at 1007.665 kb on + strand at 1007.692 kb on + strand at 1007.852 kb on + strand, within BWI76_RS04825 at 1007.862 kb on + strand, within BWI76_RS04825 at 1007.862 kb on + strand, within BWI76_RS04825 at 1007.903 kb on - strand, within BWI76_RS04825 at 1007.967 kb on + strand at 1007.983 kb on - strand, within BWI76_RS04825 at 1008.029 kb on + strand, within BWI76_RS04825 at 1008.037 kb on - strand, within BWI76_RS04825 at 1008.331 kb on - strand, within BWI76_RS04825
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL remove 1,006,038 - +0.4 1,006,523 + BWI76_RS04815 0.67 -0.8 1,006,583 + BWI76_RS04815 0.75 -1.7 1,006,613 - BWI76_RS04815 0.79 +0.3 1,006,623 - BWI76_RS04815 0.80 -2.6 1,007,616 - +0.3 1,007,661 + -1.0 1,007,665 + -0.1 1,007,692 + +0.0 1,007,852 + BWI76_RS04825 0.11 +0.1 1,007,862 + BWI76_RS04825 0.12 +2.1 1,007,862 + BWI76_RS04825 0.12 -0.7 1,007,903 - BWI76_RS04825 0.16 +1.7 1,007,967 + +1.1 1,007,983 - BWI76_RS04825 0.24 -0.4 1,008,029 + BWI76_RS04825 0.28 -1.3 1,008,037 - BWI76_RS04825 0.29 -2.7 1,008,331 - BWI76_RS04825 0.57 +0.5
Or see this region's nucleotide sequence