Experiment: LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS03570 and BWI76_RS03575 are separated by 56 nucleotides BWI76_RS03575 and BWI76_RS03580 are separated by 25 nucleotides
BWI76_RS03570: BWI76_RS03570 - choline TMA-lyase-activating enzyme, at 738,456 to 739,397
_RS03570
BWI76_RS03575: BWI76_RS03575 - choline trimethylamine-lyase, at 739,454 to 742,840
_RS03575
BWI76_RS03580: BWI76_RS03580 - iron-containing alcohol dehydrogenase, at 742,866 to 744,008
_RS03580
Position (kb) 739
740
741
742
743 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 738.513 kb on + strand at 738.513 kb on + strand at 738.513 kb on + strand at 738.513 kb on + strand at 738.521 kb on - strand at 738.553 kb on + strand, within BWI76_RS03570 at 738.553 kb on + strand, within BWI76_RS03570 at 738.561 kb on - strand, within BWI76_RS03570 at 738.561 kb on - strand, within BWI76_RS03570 at 738.592 kb on + strand, within BWI76_RS03570 at 738.596 kb on + strand, within BWI76_RS03570 at 738.604 kb on - strand, within BWI76_RS03570 at 738.676 kb on + strand, within BWI76_RS03570 at 738.697 kb on - strand at 738.744 kb on - strand, within BWI76_RS03570 at 738.748 kb on + strand, within BWI76_RS03570 at 738.757 kb on - strand, within BWI76_RS03570 at 738.757 kb on - strand, within BWI76_RS03570 at 738.801 kb on - strand, within BWI76_RS03570 at 738.846 kb on - strand, within BWI76_RS03570 at 738.846 kb on - strand, within BWI76_RS03570 at 738.875 kb on + strand, within BWI76_RS03570 at 738.880 kb on + strand, within BWI76_RS03570 at 738.886 kb on - strand, within BWI76_RS03570 at 738.904 kb on + strand, within BWI76_RS03570 at 738.904 kb on + strand, within BWI76_RS03570 at 738.909 kb on - strand, within BWI76_RS03570 at 738.912 kb on - strand, within BWI76_RS03570 at 738.912 kb on - strand, within BWI76_RS03570 at 738.912 kb on - strand, within BWI76_RS03570 at 738.912 kb on - strand, within BWI76_RS03570 at 738.945 kb on + strand, within BWI76_RS03570 at 738.962 kb on + strand, within BWI76_RS03570 at 738.962 kb on + strand, within BWI76_RS03570 at 739.028 kb on - strand, within BWI76_RS03570 at 739.043 kb on - strand, within BWI76_RS03570 at 739.066 kb on - strand, within BWI76_RS03570 at 739.098 kb on - strand, within BWI76_RS03570 at 739.098 kb on - strand, within BWI76_RS03570 at 739.098 kb on - strand, within BWI76_RS03570 at 739.099 kb on + strand, within BWI76_RS03570 at 739.113 kb on - strand, within BWI76_RS03570 at 739.195 kb on - strand, within BWI76_RS03570 at 739.199 kb on + strand, within BWI76_RS03570 at 739.207 kb on - strand, within BWI76_RS03570 at 739.207 kb on - strand, within BWI76_RS03570 at 739.207 kb on - strand, within BWI76_RS03570 at 739.207 kb on - strand, within BWI76_RS03570 at 739.207 kb on - strand, within BWI76_RS03570 at 739.207 kb on - strand, within BWI76_RS03570 at 739.207 kb on - strand, within BWI76_RS03570 at 739.233 kb on - strand, within BWI76_RS03570 at 739.233 kb on - strand, within BWI76_RS03570 at 739.246 kb on + strand, within BWI76_RS03570 at 739.267 kb on + strand, within BWI76_RS03570 at 739.268 kb on - strand, within BWI76_RS03570 at 739.275 kb on - strand, within BWI76_RS03570 at 739.275 kb on - strand, within BWI76_RS03570 at 739.275 kb on - strand, within BWI76_RS03570 at 739.275 kb on - strand, within BWI76_RS03570 at 739.275 kb on - strand, within BWI76_RS03570 at 739.417 kb on + strand at 739.421 kb on + strand at 739.421 kb on + strand at 739.426 kb on + strand at 739.426 kb on + strand at 739.426 kb on + strand at 739.429 kb on - strand at 739.434 kb on - strand at 739.434 kb on - strand at 739.450 kb on - strand at 739.500 kb on + strand at 739.500 kb on + strand at 739.500 kb on + strand at 739.503 kb on - strand at 739.508 kb on - strand at 739.508 kb on - strand at 739.508 kb on - strand at 739.510 kb on + strand at 739.510 kb on + strand at 739.510 kb on + strand at 739.510 kb on + strand at 739.518 kb on - strand at 739.518 kb on - strand at 739.518 kb on - strand at 739.518 kb on - strand at 739.518 kb on - strand at 739.524 kb on + strand at 739.534 kb on - strand at 739.652 kb on + strand at 739.669 kb on + strand at 739.706 kb on - strand at 739.812 kb on - strand, within BWI76_RS03575 at 739.860 kb on + strand, within BWI76_RS03575 at 739.918 kb on + strand, within BWI76_RS03575 at 739.926 kb on - strand, within BWI76_RS03575 at 739.931 kb on - strand, within BWI76_RS03575 at 739.940 kb on - strand, within BWI76_RS03575 at 739.968 kb on + strand, within BWI76_RS03575 at 739.995 kb on - strand, within BWI76_RS03575 at 740.521 kb on + strand, within BWI76_RS03575 at 740.641 kb on - strand, within BWI76_RS03575 at 740.666 kb on + strand, within BWI76_RS03575 at 740.710 kb on + strand, within BWI76_RS03575 at 740.758 kb on - strand, within BWI76_RS03575 at 740.850 kb on + strand, within BWI76_RS03575 at 740.876 kb on + strand, within BWI76_RS03575 at 740.907 kb on - strand, within BWI76_RS03575 at 740.920 kb on - strand, within BWI76_RS03575 at 740.981 kb on - strand, within BWI76_RS03575 at 740.983 kb on + strand, within BWI76_RS03575 at 741.052 kb on + strand, within BWI76_RS03575 at 741.120 kb on - strand, within BWI76_RS03575 at 741.144 kb on - strand, within BWI76_RS03575 at 741.329 kb on + strand, within BWI76_RS03575 at 741.428 kb on + strand, within BWI76_RS03575 at 741.436 kb on - strand, within BWI76_RS03575 at 741.436 kb on - strand, within BWI76_RS03575 at 741.545 kb on - strand, within BWI76_RS03575 at 741.552 kb on - strand, within BWI76_RS03575 at 741.787 kb on + strand, within BWI76_RS03575 at 741.819 kb on + strand, within BWI76_RS03575 at 741.997 kb on - strand, within BWI76_RS03575 at 742.279 kb on - strand, within BWI76_RS03575 at 742.322 kb on - strand, within BWI76_RS03575 at 742.612 kb on + strand at 742.819 kb on + strand at 742.819 kb on + strand at 742.827 kb on - strand at 742.827 kb on - strand at 742.841 kb on + strand at 742.841 kb on + strand at 742.841 kb on + strand at 742.888 kb on + strand at 743.040 kb on + strand, within BWI76_RS03580 at 743.073 kb on - strand, within BWI76_RS03580 at 743.102 kb on - strand, within BWI76_RS03580 at 743.292 kb on + strand, within BWI76_RS03580 at 743.655 kb on + strand, within BWI76_RS03580 at 743.685 kb on + strand, within BWI76_RS03580
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL remove 738,513 + -0.1 738,513 + +0.6 738,513 + +0.5 738,513 + +0.9 738,521 - -0.1 738,553 + BWI76_RS03570 0.10 +0.0 738,553 + BWI76_RS03570 0.10 +0.8 738,561 - BWI76_RS03570 0.11 -3.4 738,561 - BWI76_RS03570 0.11 -0.1 738,592 + BWI76_RS03570 0.14 +0.4 738,596 + BWI76_RS03570 0.15 +1.0 738,604 - BWI76_RS03570 0.16 +0.4 738,676 + BWI76_RS03570 0.23 -0.1 738,697 - +3.8 738,744 - BWI76_RS03570 0.31 -0.5 738,748 + BWI76_RS03570 0.31 -1.9 738,757 - BWI76_RS03570 0.32 +0.2 738,757 - BWI76_RS03570 0.32 +0.8 738,801 - BWI76_RS03570 0.37 -0.4 738,846 - BWI76_RS03570 0.41 -0.7 738,846 - BWI76_RS03570 0.41 +0.1 738,875 + BWI76_RS03570 0.44 -2.0 738,880 + BWI76_RS03570 0.45 -0.3 738,886 - BWI76_RS03570 0.46 -1.1 738,904 + BWI76_RS03570 0.48 +1.2 738,904 + BWI76_RS03570 0.48 +0.5 738,909 - BWI76_RS03570 0.48 +1.2 738,912 - BWI76_RS03570 0.48 +0.2 738,912 - BWI76_RS03570 0.48 +1.3 738,912 - BWI76_RS03570 0.48 -0.2 738,912 - BWI76_RS03570 0.48 +0.5 738,945 + BWI76_RS03570 0.52 +0.2 738,962 + BWI76_RS03570 0.54 +0.0 738,962 + BWI76_RS03570 0.54 -1.0 739,028 - BWI76_RS03570 0.61 +0.3 739,043 - BWI76_RS03570 0.62 +0.3 739,066 - BWI76_RS03570 0.65 -0.3 739,098 - BWI76_RS03570 0.68 +0.4 739,098 - BWI76_RS03570 0.68 -0.2 739,098 - BWI76_RS03570 0.68 -0.3 739,099 + BWI76_RS03570 0.68 +0.8 739,113 - BWI76_RS03570 0.70 -0.9 739,195 - BWI76_RS03570 0.78 +1.0 739,199 + BWI76_RS03570 0.79 +0.3 739,207 - BWI76_RS03570 0.80 -1.3 739,207 - BWI76_RS03570 0.80 -0.1 739,207 - BWI76_RS03570 0.80 +0.5 739,207 - BWI76_RS03570 0.80 +0.3 739,207 - BWI76_RS03570 0.80 -0.6 739,207 - BWI76_RS03570 0.80 -1.1 739,207 - BWI76_RS03570 0.80 +0.5 739,233 - BWI76_RS03570 0.82 -1.1 739,233 - BWI76_RS03570 0.82 -0.6 739,246 + BWI76_RS03570 0.84 -0.5 739,267 + BWI76_RS03570 0.86 +0.7 739,268 - BWI76_RS03570 0.86 +1.2 739,275 - BWI76_RS03570 0.87 +0.9 739,275 - BWI76_RS03570 0.87 +0.3 739,275 - BWI76_RS03570 0.87 +0.6 739,275 - BWI76_RS03570 0.87 -1.3 739,275 - BWI76_RS03570 0.87 +0.1 739,417 + +0.4 739,421 + -0.7 739,421 + -2.1 739,426 + -0.7 739,426 + -1.8 739,426 + +1.0 739,429 - +0.7 739,434 - -0.5 739,434 - +0.5 739,450 - +2.0 739,500 + +0.5 739,500 + -0.2 739,500 + +0.5 739,503 - +0.5 739,508 - +0.6 739,508 - +0.6 739,508 - -0.2 739,510 + +0.9 739,510 + -1.0 739,510 + -3.8 739,510 + -1.1 739,518 - +1.3 739,518 - -0.6 739,518 - +0.3 739,518 - +1.0 739,518 - -2.0 739,524 + -0.8 739,534 - +0.4 739,652 + -0.2 739,669 + -1.9 739,706 - +2.1 739,812 - BWI76_RS03575 0.11 -4.2 739,860 + BWI76_RS03575 0.12 -2.6 739,918 + BWI76_RS03575 0.14 +0.2 739,926 - BWI76_RS03575 0.14 +0.5 739,931 - BWI76_RS03575 0.14 +0.1 739,940 - BWI76_RS03575 0.14 +1.3 739,968 + BWI76_RS03575 0.15 -2.2 739,995 - BWI76_RS03575 0.16 +0.1 740,521 + BWI76_RS03575 0.32 -0.7 740,641 - BWI76_RS03575 0.35 -0.7 740,666 + BWI76_RS03575 0.36 -1.1 740,710 + BWI76_RS03575 0.37 +0.0 740,758 - BWI76_RS03575 0.39 -0.7 740,850 + BWI76_RS03575 0.41 +2.5 740,876 + BWI76_RS03575 0.42 +1.1 740,907 - BWI76_RS03575 0.43 +0.3 740,920 - BWI76_RS03575 0.43 -0.4 740,981 - BWI76_RS03575 0.45 -0.5 740,983 + BWI76_RS03575 0.45 -0.7 741,052 + BWI76_RS03575 0.47 -1.3 741,120 - BWI76_RS03575 0.49 -1.1 741,144 - BWI76_RS03575 0.50 +0.7 741,329 + BWI76_RS03575 0.55 -0.8 741,428 + BWI76_RS03575 0.58 -1.9 741,436 - BWI76_RS03575 0.59 +0.5 741,436 - BWI76_RS03575 0.59 -0.3 741,545 - BWI76_RS03575 0.62 +0.5 741,552 - BWI76_RS03575 0.62 -0.6 741,787 + BWI76_RS03575 0.69 -1.7 741,819 + BWI76_RS03575 0.70 -0.8 741,997 - BWI76_RS03575 0.75 +0.6 742,279 - BWI76_RS03575 0.83 +1.2 742,322 - BWI76_RS03575 0.85 +0.9 742,612 + +0.9 742,819 + +0.6 742,819 + -0.6 742,827 - -0.2 742,827 - +0.4 742,841 + -0.0 742,841 + -1.1 742,841 + +1.3 742,888 + -0.5 743,040 + BWI76_RS03580 0.15 +0.8 743,073 - BWI76_RS03580 0.18 -0.9 743,102 - BWI76_RS03580 0.21 -0.0 743,292 + BWI76_RS03580 0.37 +0.7 743,655 + BWI76_RS03580 0.69 +1.4 743,685 + BWI76_RS03580 0.72 +0.1
Or see this region's nucleotide sequence