Experiment: LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01720 and BWI76_RS01725 are separated by 123 nucleotides BWI76_RS01725 and BWI76_RS01730 are separated by 11 nucleotides BWI76_RS01730 and BWI76_RS01735 are separated by 188 nucleotides
BWI76_RS01720: BWI76_RS01720 - L-sorbose 1-phosphate reductase (EC 1.1.1.-) <small>(from data)</small>, at 352,507 to 353,736
_RS01720
BWI76_RS01725: BWI76_RS01725 - PTS mannose/fructose/sorbose family transporter subunit IID, at 353,860 to 354,684
_RS01725
BWI76_RS01730: BWI76_RS01730 - PTS mannose/fructose/sorbose transporter subunit IIC, at 354,696 to 355,493
_RS01730
BWI76_RS01735: BWI76_RS01735 - PTS mannose/fructose/sorbose family transporter subunit IIB, at 355,682 to 356,176
_RS01735
Position (kb) 353
354
355 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 352.930 kb on + strand, within BWI76_RS01720 at 353.173 kb on + strand, within BWI76_RS01720 at 353.189 kb on + strand, within BWI76_RS01720 at 353.450 kb on - strand, within BWI76_RS01720 at 353.467 kb on + strand, within BWI76_RS01720 at 353.467 kb on + strand, within BWI76_RS01720 at 353.721 kb on + strand at 353.748 kb on + strand at 353.815 kb on + strand at 353.942 kb on - strand at 353.951 kb on + strand, within BWI76_RS01725 at 353.978 kb on - strand, within BWI76_RS01725 at 353.985 kb on + strand, within BWI76_RS01725 at 353.990 kb on + strand, within BWI76_RS01725 at 354.077 kb on - strand, within BWI76_RS01725 at 354.134 kb on - strand, within BWI76_RS01725 at 354.182 kb on + strand, within BWI76_RS01725 at 354.223 kb on + strand, within BWI76_RS01725 at 354.335 kb on + strand, within BWI76_RS01725 at 354.477 kb on + strand, within BWI76_RS01725 at 354.718 kb on - strand at 354.718 kb on - strand at 354.769 kb on - strand at 354.784 kb on - strand, within BWI76_RS01730 at 354.786 kb on - strand, within BWI76_RS01730 at 354.801 kb on - strand, within BWI76_RS01730 at 354.905 kb on + strand, within BWI76_RS01730 at 354.926 kb on + strand, within BWI76_RS01730 at 355.001 kb on - strand, within BWI76_RS01730 at 355.001 kb on - strand, within BWI76_RS01730 at 355.008 kb on + strand, within BWI76_RS01730 at 355.057 kb on - strand, within BWI76_RS01730 at 355.114 kb on + strand, within BWI76_RS01730 at 355.148 kb on + strand, within BWI76_RS01730 at 355.148 kb on + strand, within BWI76_RS01730 at 355.156 kb on - strand, within BWI76_RS01730 at 355.169 kb on + strand, within BWI76_RS01730 at 355.178 kb on - strand, within BWI76_RS01730 at 355.253 kb on + strand, within BWI76_RS01730 at 355.263 kb on + strand, within BWI76_RS01730 at 355.301 kb on + strand, within BWI76_RS01730 at 355.320 kb on - strand, within BWI76_RS01730 at 355.322 kb on + strand, within BWI76_RS01730 at 355.322 kb on + strand, within BWI76_RS01730 at 355.322 kb on + strand, within BWI76_RS01730 at 355.330 kb on - strand, within BWI76_RS01730 at 355.330 kb on - strand, within BWI76_RS01730 at 355.330 kb on - strand, within BWI76_RS01730 at 355.330 kb on - strand, within BWI76_RS01730 at 355.330 kb on - strand, within BWI76_RS01730 at 355.330 kb on - strand, within BWI76_RS01730 at 355.340 kb on + strand, within BWI76_RS01730 at 355.340 kb on + strand, within BWI76_RS01730 at 355.348 kb on - strand, within BWI76_RS01730 at 355.348 kb on - strand, within BWI76_RS01730 at 355.348 kb on - strand, within BWI76_RS01730 at 355.367 kb on + strand, within BWI76_RS01730 at 355.367 kb on + strand, within BWI76_RS01730 at 355.375 kb on - strand, within BWI76_RS01730 at 355.375 kb on - strand, within BWI76_RS01730 at 355.382 kb on + strand, within BWI76_RS01730 at 355.382 kb on + strand, within BWI76_RS01730 at 355.390 kb on - strand, within BWI76_RS01730 at 355.390 kb on - strand at 355.390 kb on - strand, within BWI76_RS01730 at 355.390 kb on - strand, within BWI76_RS01730 at 355.393 kb on + strand, within BWI76_RS01730 at 355.400 kb on + strand, within BWI76_RS01730 at 355.408 kb on + strand, within BWI76_RS01730 at 355.408 kb on + strand, within BWI76_RS01730 at 355.408 kb on + strand, within BWI76_RS01730 at 355.408 kb on + strand, within BWI76_RS01730 at 355.408 kb on + strand, within BWI76_RS01730 at 355.408 kb on - strand, within BWI76_RS01730 at 355.414 kb on + strand at 355.416 kb on - strand at 355.416 kb on - strand at 355.416 kb on - strand at 355.416 kb on - strand at 355.421 kb on - strand at 355.425 kb on - strand at 355.429 kb on + strand at 355.432 kb on - strand at 355.434 kb on - strand at 355.437 kb on + strand at 355.439 kb on + strand at 355.509 kb on + strand at 355.509 kb on + strand at 355.509 kb on + strand at 355.510 kb on + strand at 355.511 kb on + strand at 355.511 kb on - strand at 355.517 kb on - strand at 355.517 kb on - strand at 355.517 kb on - strand at 355.519 kb on - strand at 355.537 kb on - strand at 355.545 kb on - strand at 355.546 kb on + strand at 355.546 kb on - strand at 355.548 kb on + strand at 355.548 kb on + strand at 355.548 kb on + strand at 355.550 kb on + strand at 355.550 kb on + strand at 355.550 kb on + strand at 355.550 kb on + strand at 355.550 kb on + strand at 355.550 kb on + strand at 355.550 kb on + strand at 355.550 kb on + strand at 355.550 kb on + strand at 355.550 kb on + strand at 355.550 kb on + strand at 355.550 kb on + strand at 355.550 kb on + strand at 355.550 kb on + strand at 355.550 kb on + strand at 355.550 kb on + strand at 355.550 kb on + strand at 355.550 kb on + strand at 355.550 kb on + strand at 355.550 kb on + strand at 355.550 kb on + strand at 355.550 kb on + strand at 355.550 kb on + strand at 355.550 kb on + strand at 355.550 kb on + strand at 355.550 kb on + strand at 355.550 kb on + strand at 355.550 kb on + strand at 355.556 kb on - strand at 355.556 kb on - strand at 355.556 kb on - strand at 355.556 kb on - strand at 355.558 kb on - strand at 355.558 kb on - strand at 355.558 kb on - strand at 355.558 kb on - strand at 355.558 kb on - strand at 355.558 kb on - strand at 355.558 kb on - strand at 355.558 kb on - strand at 355.558 kb on - strand at 355.558 kb on - strand at 355.558 kb on - strand at 355.558 kb on - strand at 355.558 kb on - strand at 355.558 kb on - strand at 355.558 kb on - strand at 355.558 kb on - strand at 355.558 kb on - strand at 355.558 kb on - strand at 355.558 kb on - strand at 355.558 kb on - strand at 355.558 kb on - strand at 355.558 kb on - strand at 355.558 kb on - strand at 355.558 kb on - strand at 355.558 kb on - strand at 355.558 kb on - strand at 355.560 kb on + strand at 355.570 kb on + strand at 355.575 kb on + strand at 355.585 kb on + strand at 355.591 kb on + strand at 355.595 kb on + strand at 355.599 kb on + strand at 355.599 kb on - strand at 355.604 kb on - strand at 355.609 kb on + strand at 355.609 kb on - strand at 355.611 kb on + strand at 355.617 kb on - strand at 355.619 kb on - strand at 355.619 kb on - strand at 355.619 kb on - strand at 355.642 kb on + strand at 355.646 kb on - strand at 355.648 kb on + strand at 355.656 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL remove 352,930 + BWI76_RS01720 0.34 -0.4 353,173 + BWI76_RS01720 0.54 +0.1 353,189 + BWI76_RS01720 0.55 +0.1 353,450 - BWI76_RS01720 0.77 -0.2 353,467 + BWI76_RS01720 0.78 -0.1 353,467 + BWI76_RS01720 0.78 -0.3 353,721 + -1.3 353,748 + +2.5 353,815 + -0.1 353,942 - +1.3 353,951 + BWI76_RS01725 0.11 +1.3 353,978 - BWI76_RS01725 0.14 +0.7 353,985 + BWI76_RS01725 0.15 +1.1 353,990 + BWI76_RS01725 0.16 +0.3 354,077 - BWI76_RS01725 0.26 -0.3 354,134 - BWI76_RS01725 0.33 -2.2 354,182 + BWI76_RS01725 0.39 +1.7 354,223 + BWI76_RS01725 0.44 +0.3 354,335 + BWI76_RS01725 0.58 +0.1 354,477 + BWI76_RS01725 0.75 +1.1 354,718 - -0.1 354,718 - +1.3 354,769 - -1.0 354,784 - BWI76_RS01730 0.11 -0.8 354,786 - BWI76_RS01730 0.11 +0.2 354,801 - BWI76_RS01730 0.13 +0.8 354,905 + BWI76_RS01730 0.26 +0.3 354,926 + BWI76_RS01730 0.29 -0.8 355,001 - BWI76_RS01730 0.38 -1.9 355,001 - BWI76_RS01730 0.38 -1.2 355,008 + BWI76_RS01730 0.39 -0.2 355,057 - BWI76_RS01730 0.45 +0.3 355,114 + BWI76_RS01730 0.52 -0.1 355,148 + BWI76_RS01730 0.57 -0.2 355,148 + BWI76_RS01730 0.57 +0.5 355,156 - BWI76_RS01730 0.58 +0.3 355,169 + BWI76_RS01730 0.59 -0.2 355,178 - BWI76_RS01730 0.60 +0.9 355,253 + BWI76_RS01730 0.70 -0.7 355,263 + BWI76_RS01730 0.71 +0.1 355,301 + BWI76_RS01730 0.76 -0.5 355,320 - BWI76_RS01730 0.78 -0.4 355,322 + BWI76_RS01730 0.78 +1.3 355,322 + BWI76_RS01730 0.78 -1.0 355,322 + BWI76_RS01730 0.78 +1.2 355,330 - BWI76_RS01730 0.79 -0.9 355,330 - BWI76_RS01730 0.79 -2.2 355,330 - BWI76_RS01730 0.79 +0.1 355,330 - BWI76_RS01730 0.79 +1.3 355,330 - BWI76_RS01730 0.79 -1.1 355,330 - BWI76_RS01730 0.79 +0.4 355,340 + BWI76_RS01730 0.81 -1.5 355,340 + BWI76_RS01730 0.81 -0.3 355,348 - BWI76_RS01730 0.82 -0.3 355,348 - BWI76_RS01730 0.82 -0.3 355,348 - BWI76_RS01730 0.82 -0.9 355,367 + BWI76_RS01730 0.84 -0.3 355,367 + BWI76_RS01730 0.84 +0.3 355,375 - BWI76_RS01730 0.85 +0.4 355,375 - BWI76_RS01730 0.85 -1.0 355,382 + BWI76_RS01730 0.86 -1.9 355,382 + BWI76_RS01730 0.86 +0.0 355,390 - BWI76_RS01730 0.87 -0.2 355,390 - +1.3 355,390 - BWI76_RS01730 0.87 +0.9 355,390 - BWI76_RS01730 0.87 +0.2 355,393 + BWI76_RS01730 0.87 +0.5 355,400 + BWI76_RS01730 0.88 -1.4 355,408 + BWI76_RS01730 0.89 -2.0 355,408 + BWI76_RS01730 0.89 -1.3 355,408 + BWI76_RS01730 0.89 +1.9 355,408 + BWI76_RS01730 0.89 +0.5 355,408 + BWI76_RS01730 0.89 +2.0 355,408 - BWI76_RS01730 0.89 +0.3 355,414 + +0.6 355,416 - +0.1 355,416 - +0.6 355,416 - -2.5 355,416 - -0.2 355,421 - -0.6 355,425 - +0.5 355,429 + -0.7 355,432 - -0.5 355,434 - +2.0 355,437 + +1.2 355,439 + -1.5 355,509 + -0.6 355,509 + -0.0 355,509 + +0.9 355,510 + +0.7 355,511 + -0.3 355,511 - -2.1 355,517 - -1.5 355,517 - +0.7 355,517 - +0.1 355,519 - +0.1 355,537 - +0.2 355,545 - +0.3 355,546 + +0.6 355,546 - -1.5 355,548 + +0.6 355,548 + -1.3 355,548 + +1.3 355,550 + -0.5 355,550 + +1.3 355,550 + +0.7 355,550 + +0.1 355,550 + -0.7 355,550 + -0.4 355,550 + +1.1 355,550 + -0.1 355,550 + +0.3 355,550 + -1.5 355,550 + -0.8 355,550 + -0.1 355,550 + -0.7 355,550 + +0.5 355,550 + +1.3 355,550 + -0.8 355,550 + -0.4 355,550 + +1.6 355,550 + -0.3 355,550 + -0.4 355,550 + +1.0 355,550 + -1.2 355,550 + -1.0 355,550 + -2.7 355,550 + +0.2 355,550 + +0.5 355,550 + +0.9 355,550 + +0.3 355,556 - +0.7 355,556 - -0.4 355,556 - -0.6 355,556 - +0.7 355,558 - -2.2 355,558 - +0.2 355,558 - +0.3 355,558 - +1.3 355,558 - +1.9 355,558 - -3.0 355,558 - +0.4 355,558 - -1.3 355,558 - +0.0 355,558 - +0.6 355,558 - -0.0 355,558 - -1.1 355,558 - -0.4 355,558 - -0.7 355,558 - +1.1 355,558 - +0.3 355,558 - +0.3 355,558 - +0.6 355,558 - +1.6 355,558 - +1.6 355,558 - -1.5 355,558 - -0.1 355,558 - -1.7 355,558 - +0.1 355,558 - +0.3 355,558 - +0.9 355,560 + -1.0 355,570 + -0.7 355,575 + +0.5 355,585 + +1.2 355,591 + -0.3 355,595 + -1.7 355,599 + +1.9 355,599 - -0.1 355,604 - +2.1 355,609 + -1.4 355,609 - -3.0 355,611 + -0.7 355,617 - +0.5 355,619 - -1.5 355,619 - -1.2 355,619 - -0.2 355,642 + +1.9 355,646 - +0.3 355,648 + -0.8 355,656 - -0.4
Or see this region's nucleotide sequence