Experiment: LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01225 and BWI76_RS01230 are separated by 43 nucleotides BWI76_RS01230 and BWI76_RS01235 are separated by 49 nucleotides BWI76_RS01235 and BWI76_RS01240 are separated by 155 nucleotides
BWI76_RS01225: BWI76_RS01225 - magnesium transporter CorA, at 251,098 to 252,048
_RS01225
BWI76_RS01230: BWI76_RS01230 - chloramphenicol resistance permease RarD, at 252,092 to 252,985
_RS01230
BWI76_RS01235: BWI76_RS01235 - hypothetical protein, at 253,035 to 253,499
_RS01235
BWI76_RS01240: BWI76_RS01240 - phospholipase A, at 253,655 to 254,527
_RS01240
Position (kb) 252
253 Strain fitness (log2 ratio) -1
0
1
2 at 251.188 kb on - strand at 251.236 kb on + strand, within BWI76_RS01225 at 251.255 kb on + strand, within BWI76_RS01225 at 251.298 kb on + strand, within BWI76_RS01225 at 251.366 kb on + strand, within BWI76_RS01225 at 251.414 kb on + strand, within BWI76_RS01225 at 251.547 kb on + strand, within BWI76_RS01225 at 251.929 kb on - strand, within BWI76_RS01225 at 251.940 kb on + strand, within BWI76_RS01225 at 252.165 kb on - strand at 252.262 kb on + strand, within BWI76_RS01230 at 252.265 kb on - strand, within BWI76_RS01230 at 252.287 kb on - strand, within BWI76_RS01230 at 252.549 kb on + strand, within BWI76_RS01230 at 252.557 kb on - strand, within BWI76_RS01230 at 252.557 kb on - strand, within BWI76_RS01230 at 253.077 kb on - strand at 253.154 kb on + strand, within BWI76_RS01235 at 253.273 kb on + strand, within BWI76_RS01235 at 253.538 kb on - strand at 253.615 kb on - strand at 253.657 kb on + strand at 253.664 kb on + strand at 253.664 kb on + strand at 253.672 kb on - strand at 253.672 kb on - strand at 253.743 kb on + strand, within BWI76_RS01240 at 253.815 kb on + strand, within BWI76_RS01240 at 253.823 kb on - strand, within BWI76_RS01240 at 253.939 kb on - strand, within BWI76_RS01240
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL remove 251,188 - +0.1 251,236 + BWI76_RS01225 0.15 +1.5 251,255 + BWI76_RS01225 0.17 +0.3 251,298 + BWI76_RS01225 0.21 -0.2 251,366 + BWI76_RS01225 0.28 +0.3 251,414 + BWI76_RS01225 0.33 -0.7 251,547 + BWI76_RS01225 0.47 +0.4 251,929 - BWI76_RS01225 0.87 +0.7 251,940 + BWI76_RS01225 0.89 +0.3 252,165 - -1.1 252,262 + BWI76_RS01230 0.19 -0.4 252,265 - BWI76_RS01230 0.19 +1.1 252,287 - BWI76_RS01230 0.22 -0.0 252,549 + BWI76_RS01230 0.51 +2.2 252,557 - BWI76_RS01230 0.52 +0.2 252,557 - BWI76_RS01230 0.52 +1.0 253,077 - +0.6 253,154 + BWI76_RS01235 0.26 +0.4 253,273 + BWI76_RS01235 0.51 +1.0 253,538 - -0.3 253,615 - -0.7 253,657 + -0.0 253,664 + +0.7 253,664 + +0.5 253,672 - +0.7 253,672 - +0.6 253,743 + BWI76_RS01240 0.10 +0.5 253,815 + BWI76_RS01240 0.18 +1.2 253,823 - BWI76_RS01240 0.19 +0.4 253,939 - BWI76_RS01240 0.33 +0.1
Or see this region's nucleotide sequence