Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS01025

Experiment: LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS01020 and BWI76_RS01025 are separated by 3 nucleotidesBWI76_RS01025 and BWI76_RS01030 overlap by 17 nucleotides BWI76_RS01020: BWI76_RS01020 - DNA helicase Rep, at 207,493 to 209,511 _RS01020 BWI76_RS01025: BWI76_RS01025 - hypothetical protein, at 209,515 to 210,477 _RS01025 BWI76_RS01030: BWI76_RS01030 - cytosine/purine, uracil, thiamine, allantoin permease family protein, at 210,461 to 211,876 _RS01030 Position (kb) 209 210 211Strain fitness (log2 ratio) -2 -1 0 1at 209.478 kb on + strandat 209.635 kb on + strand, within BWI76_RS01025at 209.705 kb on + strand, within BWI76_RS01025at 209.713 kb on - strand, within BWI76_RS01025at 209.829 kb on - strand, within BWI76_RS01025at 209.848 kb on + strand, within BWI76_RS01025at 209.865 kb on - strand, within BWI76_RS01025at 209.865 kb on - strand, within BWI76_RS01025at 209.865 kb on - strand, within BWI76_RS01025at 210.021 kb on + strand, within BWI76_RS01025at 210.031 kb on + strand, within BWI76_RS01025at 210.034 kb on - strand, within BWI76_RS01025at 210.065 kb on + strand, within BWI76_RS01025at 210.128 kb on - strand, within BWI76_RS01025at 210.128 kb on - strand, within BWI76_RS01025at 210.462 kb on - strandat 210.549 kb on + strandat 210.649 kb on + strand, within BWI76_RS01030at 210.652 kb on - strand, within BWI76_RS01030at 210.662 kb on - strand, within BWI76_RS01030at 210.677 kb on + strand, within BWI76_RS01030at 210.757 kb on + strand, within BWI76_RS01030at 211.104 kb on + strand, within BWI76_RS01030at 211.256 kb on - strand, within BWI76_RS01030at 211.441 kb on + strand, within BWI76_RS01030

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Per-strain Table

Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
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209,478 + -2.2
209,635 + BWI76_RS01025 0.12 -0.3
209,705 + BWI76_RS01025 0.20 -2.5
209,713 - BWI76_RS01025 0.21 +1.3
209,829 - BWI76_RS01025 0.33 +1.1
209,848 + BWI76_RS01025 0.35 -0.2
209,865 - BWI76_RS01025 0.36 -0.0
209,865 - BWI76_RS01025 0.36 +0.3
209,865 - BWI76_RS01025 0.36 -2.3
210,021 + BWI76_RS01025 0.53 +0.7
210,031 + BWI76_RS01025 0.54 +1.0
210,034 - BWI76_RS01025 0.54 +1.3
210,065 + BWI76_RS01025 0.57 +0.7
210,128 - BWI76_RS01025 0.64 -1.5
210,128 - BWI76_RS01025 0.64 +0.4
210,462 - +1.3
210,549 + -0.0
210,649 + BWI76_RS01030 0.13 +1.5
210,652 - BWI76_RS01030 0.13 -1.1
210,662 - BWI76_RS01030 0.14 +0.7
210,677 + BWI76_RS01030 0.15 +0.1
210,757 + BWI76_RS01030 0.21 +0.3
211,104 + BWI76_RS01030 0.45 -0.3
211,256 - BWI76_RS01030 0.56 +0.6
211,441 + BWI76_RS01030 0.69 +0.4

Or see this region's nucleotide sequence