Experiment: LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00410 and BWI76_RS00415 are separated by 133 nucleotides BWI76_RS00415 and BWI76_RS00420 overlap by 7 nucleotides BWI76_RS00420 and BWI76_RS00425 overlap by 4 nucleotides
BWI76_RS00410: BWI76_RS00410 - translational GTPase TypA, at 85,322 to 87,145
_RS00410
BWI76_RS00415: BWI76_RS00415 - glucose-1-phosphatase, at 87,279 to 87,878
_RS00415
BWI76_RS00420: BWI76_RS00420 - hypothetical protein, at 87,872 to 88,732
_RS00420
BWI76_RS00425: BWI76_RS00425 - D-tyrosyl-tRNA(Tyr) deacylase, at 88,729 to 89,166
_RS00425
Position (kb) 87
88 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 87.087 kb on + strand at 87.269 kb on - strand at 87.414 kb on - strand, within BWI76_RS00415 at 87.492 kb on + strand, within BWI76_RS00415 at 87.523 kb on + strand, within BWI76_RS00415 at 87.543 kb on - strand, within BWI76_RS00415 at 87.604 kb on - strand, within BWI76_RS00415 at 87.614 kb on + strand, within BWI76_RS00415 at 87.772 kb on - strand, within BWI76_RS00415 at 87.796 kb on + strand, within BWI76_RS00415 at 87.804 kb on - strand, within BWI76_RS00415 at 87.882 kb on - strand at 88.062 kb on + strand, within BWI76_RS00420 at 88.140 kb on + strand, within BWI76_RS00420 at 88.295 kb on - strand, within BWI76_RS00420 at 88.316 kb on - strand, within BWI76_RS00420 at 88.464 kb on + strand, within BWI76_RS00420 at 88.464 kb on - strand, within BWI76_RS00420 at 88.472 kb on - strand, within BWI76_RS00420 at 88.484 kb on - strand, within BWI76_RS00420 at 88.523 kb on + strand, within BWI76_RS00420 at 88.531 kb on - strand, within BWI76_RS00420 at 88.599 kb on - strand, within BWI76_RS00420 at 88.648 kb on + strand at 88.736 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL remove 87,087 + +1.3 87,269 - +0.9 87,414 - BWI76_RS00415 0.23 -2.2 87,492 + BWI76_RS00415 0.35 -1.5 87,523 + BWI76_RS00415 0.41 -1.3 87,543 - BWI76_RS00415 0.44 +0.0 87,604 - BWI76_RS00415 0.54 -1.3 87,614 + BWI76_RS00415 0.56 -2.0 87,772 - BWI76_RS00415 0.82 +0.9 87,796 + BWI76_RS00415 0.86 -1.5 87,804 - BWI76_RS00415 0.88 -1.3 87,882 - -0.3 88,062 + BWI76_RS00420 0.22 +1.3 88,140 + BWI76_RS00420 0.31 +1.5 88,295 - BWI76_RS00420 0.49 +1.3 88,316 - BWI76_RS00420 0.52 -0.4 88,464 + BWI76_RS00420 0.69 +0.6 88,464 - BWI76_RS00420 0.69 +0.5 88,472 - BWI76_RS00420 0.70 -0.9 88,484 - BWI76_RS00420 0.71 -1.4 88,523 + BWI76_RS00420 0.76 -2.2 88,531 - BWI76_RS00420 0.77 +0.7 88,599 - BWI76_RS00420 0.84 -2.9 88,648 + -0.2 88,736 + -0.7
Or see this region's nucleotide sequence