Experiment: LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS27040 and BWI76_RS27045 are separated by 122 nucleotides BWI76_RS27045 and BWI76_RS27050 are separated by 60 nucleotides BWI76_RS27050 and BWI76_RS27055 overlap by 59 nucleotides
BWI76_RS27040: BWI76_RS27040 - xylose ABC transporter permease, at 5,610,773 to 5,611,954
_RS27040
BWI76_RS27045: BWI76_RS27045 - XylR family transcriptional regulator, at 5,612,077 to 5,613,255
_RS27045
BWI76_RS27050: BWI76_RS27050 - hypothetical protein, at 5,613,316 to 5,614,143
_RS27050
BWI76_RS27055: BWI76_RS27055 - hypothetical protein, at 5,614,085 to 5,614,378
_RS27055
Position (kb) 5612
5613
5614 Strain fitness (log2 ratio) -2
-1
0
1 at 5611.131 kb on - strand, within BWI76_RS27040 at 5611.248 kb on + strand, within BWI76_RS27040 at 5611.267 kb on - strand, within BWI76_RS27040 at 5611.379 kb on - strand, within BWI76_RS27040 at 5611.399 kb on + strand, within BWI76_RS27040 at 5611.399 kb on + strand, within BWI76_RS27040 at 5611.695 kb on + strand, within BWI76_RS27040 at 5611.778 kb on + strand, within BWI76_RS27040 at 5611.786 kb on - strand, within BWI76_RS27040 at 5611.843 kb on + strand at 5611.843 kb on + strand at 5611.936 kb on - strand at 5611.951 kb on - strand at 5611.958 kb on - strand at 5612.000 kb on - strand at 5612.039 kb on + strand at 5612.039 kb on + strand at 5612.047 kb on - strand at 5612.049 kb on + strand at 5612.062 kb on + strand at 5612.096 kb on + strand at 5612.125 kb on + strand at 5612.133 kb on - strand at 5612.133 kb on - strand at 5612.133 kb on - strand at 5612.133 kb on - strand at 5612.163 kb on + strand at 5612.163 kb on + strand at 5612.394 kb on + strand, within BWI76_RS27045 at 5612.743 kb on - strand, within BWI76_RS27045 at 5612.743 kb on - strand, within BWI76_RS27045 at 5612.772 kb on + strand, within BWI76_RS27045 at 5612.780 kb on - strand, within BWI76_RS27045 at 5612.906 kb on - strand, within BWI76_RS27045 at 5612.954 kb on + strand, within BWI76_RS27045 at 5612.979 kb on + strand, within BWI76_RS27045 at 5612.986 kb on - strand, within BWI76_RS27045 at 5613.212 kb on + strand at 5613.214 kb on - strand at 5613.378 kb on + strand at 5613.454 kb on - strand, within BWI76_RS27050 at 5613.679 kb on - strand, within BWI76_RS27050 at 5613.698 kb on + strand, within BWI76_RS27050 at 5613.874 kb on + strand, within BWI76_RS27050 at 5613.877 kb on - strand, within BWI76_RS27050 at 5614.122 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL remove 5,611,131 - BWI76_RS27040 0.30 +0.5 5,611,248 + BWI76_RS27040 0.40 +0.8 5,611,267 - BWI76_RS27040 0.42 -2.2 5,611,379 - BWI76_RS27040 0.51 -0.6 5,611,399 + BWI76_RS27040 0.53 -0.4 5,611,399 + BWI76_RS27040 0.53 +0.4 5,611,695 + BWI76_RS27040 0.78 +0.7 5,611,778 + BWI76_RS27040 0.85 -0.5 5,611,786 - BWI76_RS27040 0.86 +0.0 5,611,843 + +0.4 5,611,843 + +1.0 5,611,936 - -0.9 5,611,951 - +1.0 5,611,958 - -0.1 5,612,000 - -0.6 5,612,039 + +0.2 5,612,039 + +0.4 5,612,047 - +0.4 5,612,049 + +1.4 5,612,062 + -0.3 5,612,096 + +0.4 5,612,125 + +0.4 5,612,133 - +0.3 5,612,133 - +1.4 5,612,133 - +1.1 5,612,133 - -0.6 5,612,163 + -0.3 5,612,163 + -1.8 5,612,394 + BWI76_RS27045 0.27 +0.2 5,612,743 - BWI76_RS27045 0.56 -1.9 5,612,743 - BWI76_RS27045 0.56 +1.2 5,612,772 + BWI76_RS27045 0.59 +0.5 5,612,780 - BWI76_RS27045 0.60 +0.4 5,612,906 - BWI76_RS27045 0.70 +0.5 5,612,954 + BWI76_RS27045 0.74 +0.6 5,612,979 + BWI76_RS27045 0.77 +1.3 5,612,986 - BWI76_RS27045 0.77 +1.0 5,613,212 + -0.6 5,613,214 - -1.2 5,613,378 + -0.3 5,613,454 - BWI76_RS27050 0.17 -0.0 5,613,679 - BWI76_RS27050 0.44 +0.1 5,613,698 + BWI76_RS27050 0.46 +0.2 5,613,874 + BWI76_RS27050 0.67 +0.3 5,613,877 - BWI76_RS27050 0.68 -0.4 5,614,122 - -0.6
Or see this region's nucleotide sequence