Experiment: LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS26000 and BWI76_RS26005 are separated by 89 nucleotides BWI76_RS26005 and BWI76_RS26010 are separated by 31 nucleotides BWI76_RS26010 and BWI76_RS26015 overlap by 11 nucleotides BWI76_RS26015 and BWI76_RS26020 are separated by 108 nucleotides
BWI76_RS26000: BWI76_RS26000 - aspartate aminotransferase family protein, at 5,373,847 to 5,375,067
_RS26000
BWI76_RS26005: BWI76_RS26005 - glutamine amidotransferase, at 5,375,157 to 5,375,720
_RS26005
BWI76_RS26010: BWI76_RS26010 - cell filamentation protein Fic, at 5,375,752 to 5,376,354
_RS26010
BWI76_RS26015: BWI76_RS26015 - DUF2559 domain-containing protein, at 5,376,344 to 5,376,511
_RS26015
BWI76_RS26020: BWI76_RS26020 - peptidylprolyl isomerase A, at 5,376,620 to 5,377,189
_RS26020
Position (kb) 5375
5376 Strain fitness (log2 ratio) -1
0
1 at 5374.262 kb on + strand, within BWI76_RS26000 at 5374.302 kb on + strand, within BWI76_RS26000 at 5374.312 kb on + strand, within BWI76_RS26000 at 5374.312 kb on + strand, within BWI76_RS26000 at 5374.315 kb on - strand, within BWI76_RS26000 at 5374.396 kb on + strand, within BWI76_RS26000 at 5374.418 kb on - strand, within BWI76_RS26000 at 5374.475 kb on - strand, within BWI76_RS26000 at 5374.649 kb on + strand, within BWI76_RS26000 at 5374.823 kb on - strand, within BWI76_RS26000 at 5374.970 kb on + strand at 5374.978 kb on - strand at 5375.294 kb on + strand, within BWI76_RS26005 at 5375.367 kb on - strand, within BWI76_RS26005 at 5375.781 kb on - strand at 5375.781 kb on - strand at 5375.850 kb on - strand, within BWI76_RS26010 at 5375.870 kb on - strand, within BWI76_RS26010 at 5375.982 kb on - strand, within BWI76_RS26010 at 5376.018 kb on + strand, within BWI76_RS26010 at 5376.137 kb on - strand, within BWI76_RS26010 at 5376.275 kb on + strand, within BWI76_RS26010 at 5376.299 kb on + strand at 5376.307 kb on - strand at 5376.388 kb on - strand, within BWI76_RS26015 at 5376.602 kb on - strand at 5376.626 kb on - strand at 5376.662 kb on - strand at 5376.679 kb on + strand, within BWI76_RS26020
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL remove 5,374,262 + BWI76_RS26000 0.34 +0.7 5,374,302 + BWI76_RS26000 0.37 -0.6 5,374,312 + BWI76_RS26000 0.38 +0.7 5,374,312 + BWI76_RS26000 0.38 +0.4 5,374,315 - BWI76_RS26000 0.38 -0.2 5,374,396 + BWI76_RS26000 0.45 -0.4 5,374,418 - BWI76_RS26000 0.47 -0.4 5,374,475 - BWI76_RS26000 0.51 -0.3 5,374,649 + BWI76_RS26000 0.66 +0.4 5,374,823 - BWI76_RS26000 0.80 -1.4 5,374,970 + -0.4 5,374,978 - +0.1 5,375,294 + BWI76_RS26005 0.24 +1.4 5,375,367 - BWI76_RS26005 0.37 +0.5 5,375,781 - +0.6 5,375,781 - -1.1 5,375,850 - BWI76_RS26010 0.16 +0.2 5,375,870 - BWI76_RS26010 0.20 +0.0 5,375,982 - BWI76_RS26010 0.38 +0.5 5,376,018 + BWI76_RS26010 0.44 -1.7 5,376,137 - BWI76_RS26010 0.64 +0.2 5,376,275 + BWI76_RS26010 0.87 +0.5 5,376,299 + +0.2 5,376,307 - +1.5 5,376,388 - BWI76_RS26015 0.26 +0.2 5,376,602 - -0.2 5,376,626 - -0.0 5,376,662 - +0.1 5,376,679 + BWI76_RS26020 0.10 -0.6
Or see this region's nucleotide sequence