Experiment: LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS23650 and BWI76_RS23655 are separated by 112 nucleotides BWI76_RS23655 and BWI76_RS23660 are separated by 47 nucleotides
BWI76_RS23650: BWI76_RS23650 - MFS transporter, at 4,935,428 to 4,936,975
_RS23650
BWI76_RS23655: BWI76_RS23655 - DeoR family transcriptional regulator, at 4,937,088 to 4,937,861
_RS23655
BWI76_RS23660: BWI76_RS23660 - pentose kinase, at 4,937,909 to 4,939,414
_RS23660
Position (kb)
4937
4938 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 4936.093 kb on + strand, within BWI76_RS23650 at 4936.101 kb on - strand, within BWI76_RS23650 at 4936.149 kb on + strand, within BWI76_RS23650 at 4936.157 kb on - strand, within BWI76_RS23650 at 4936.157 kb on - strand at 4936.157 kb on - strand, within BWI76_RS23650 at 4936.157 kb on - strand, within BWI76_RS23650 at 4936.380 kb on + strand, within BWI76_RS23650 at 4936.511 kb on - strand, within BWI76_RS23650 at 4936.582 kb on - strand, within BWI76_RS23650 at 4936.710 kb on + strand, within BWI76_RS23650 at 4936.783 kb on + strand, within BWI76_RS23650 at 4936.849 kb on + strand at 4936.882 kb on - strand at 4936.969 kb on - strand at 4936.985 kb on + strand at 4937.187 kb on - strand, within BWI76_RS23655 at 4937.187 kb on - strand, within BWI76_RS23655 at 4937.221 kb on + strand, within BWI76_RS23655 at 4937.656 kb on + strand, within BWI76_RS23655 at 4937.664 kb on - strand, within BWI76_RS23655 at 4937.713 kb on - strand, within BWI76_RS23655 at 4937.945 kb on + strand at 4937.988 kb on + strand at 4937.996 kb on - strand at 4938.292 kb on - strand, within BWI76_RS23660 at 4938.293 kb on + strand, within BWI76_RS23660 at 4938.301 kb on - strand, within BWI76_RS23660 at 4938.301 kb on - strand, within BWI76_RS23660 at 4938.529 kb on + strand, within BWI76_RS23660 at 4938.669 kb on - strand, within BWI76_RS23660 at 4938.781 kb on + strand, within BWI76_RS23660
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL remove 4,936,093 + BWI76_RS23650 0.43 -0.4 4,936,101 - BWI76_RS23650 0.43 +0.6 4,936,149 + BWI76_RS23650 0.47 -1.6 4,936,157 - BWI76_RS23650 0.47 -0.1 4,936,157 - -0.4 4,936,157 - BWI76_RS23650 0.47 +0.2 4,936,157 - BWI76_RS23650 0.47 -1.0 4,936,380 + BWI76_RS23650 0.61 +1.0 4,936,511 - BWI76_RS23650 0.70 -3.2 4,936,582 - BWI76_RS23650 0.75 +1.5 4,936,710 + BWI76_RS23650 0.83 -1.3 4,936,783 + BWI76_RS23650 0.88 -2.6 4,936,849 + +0.4 4,936,882 - -0.2 4,936,969 - -0.2 4,936,985 + +0.4 4,937,187 - BWI76_RS23655 0.13 +0.1 4,937,187 - BWI76_RS23655 0.13 -1.8 4,937,221 + BWI76_RS23655 0.17 -1.9 4,937,656 + BWI76_RS23655 0.73 -2.7 4,937,664 - BWI76_RS23655 0.74 -1.5 4,937,713 - BWI76_RS23655 0.81 -0.4 4,937,945 + +0.5 4,937,988 + +1.3 4,937,996 - -0.1 4,938,292 - BWI76_RS23660 0.25 +0.6 4,938,293 + BWI76_RS23660 0.25 -0.3 4,938,301 - BWI76_RS23660 0.26 -1.0 4,938,301 - BWI76_RS23660 0.26 +1.0 4,938,529 + BWI76_RS23660 0.41 -2.0 4,938,669 - BWI76_RS23660 0.50 +0.4 4,938,781 + BWI76_RS23660 0.58 +0.1
Or see this region's nucleotide sequence