Experiment: LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20570 and BWI76_RS20575 are separated by 259 nucleotides BWI76_RS20575 and BWI76_RS20580 are separated by 44 nucleotides BWI76_RS20580 and BWI76_RS20585 are separated by 46 nucleotides BWI76_RS20585 and BWI76_RS20590 are separated by 4 nucleotides BWI76_RS20590 and BWI76_RS20595 are separated by 89 nucleotides BWI76_RS20595 and BWI76_RS20600 are separated by 96 nucleotides
BWI76_RS20570: BWI76_RS20570 - glutamate--tRNA ligase, at 4,307,321 to 4,308,739
_RS20570
BWI76_RS20575: BWI76_RS20575 - tRNA-Val, at 4,308,999 to 4,309,074
_RS20575
BWI76_RS20580: BWI76_RS20580 - tRNA-Val, at 4,309,119 to 4,309,194
_RS20580
BWI76_RS20585: BWI76_RS20585 - tRNA-Val, at 4,309,241 to 4,309,316
_RS20585
BWI76_RS20590: BWI76_RS20590 - tRNA-Lys, at 4,309,321 to 4,309,396
_RS20590
BWI76_RS20595: BWI76_RS20595 - LysR family transcriptional regulator, at 4,309,486 to 4,310,370
_RS20595
BWI76_RS20600: BWI76_RS20600 - nucleoside permease, at 4,310,467 to 4,311,723
_RS20600
Position (kb) 4309
4310
4311 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 4309.435 kb on + strand at 4309.539 kb on + strand at 4309.766 kb on - strand, within BWI76_RS20595 at 4309.881 kb on - strand, within BWI76_RS20595 at 4309.887 kb on + strand at 4310.179 kb on + strand, within BWI76_RS20595 at 4310.179 kb on + strand, within BWI76_RS20595 at 4310.179 kb on + strand, within BWI76_RS20595 at 4310.179 kb on + strand, within BWI76_RS20595 at 4310.179 kb on + strand, within BWI76_RS20595 at 4310.187 kb on - strand, within BWI76_RS20595 at 4310.187 kb on - strand, within BWI76_RS20595 at 4310.272 kb on - strand, within BWI76_RS20595 at 4310.394 kb on + strand at 4310.414 kb on + strand at 4310.660 kb on + strand, within BWI76_RS20600 at 4310.775 kb on - strand, within BWI76_RS20600 at 4310.796 kb on + strand, within BWI76_RS20600 at 4310.804 kb on - strand, within BWI76_RS20600 at 4310.811 kb on - strand, within BWI76_RS20600 at 4310.842 kb on + strand, within BWI76_RS20600 at 4310.849 kb on + strand, within BWI76_RS20600 at 4311.241 kb on - strand, within BWI76_RS20600 at 4311.241 kb on - strand, within BWI76_RS20600 at 4311.255 kb on + strand, within BWI76_RS20600 at 4311.263 kb on - strand, within BWI76_RS20600
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL remove 4,309,435 + -3.1 4,309,539 + -0.9 4,309,766 - BWI76_RS20595 0.32 +0.4 4,309,881 - BWI76_RS20595 0.45 -3.1 4,309,887 + +1.3 4,310,179 + BWI76_RS20595 0.78 -1.0 4,310,179 + BWI76_RS20595 0.78 -0.7 4,310,179 + BWI76_RS20595 0.78 +1.4 4,310,179 + BWI76_RS20595 0.78 +0.1 4,310,179 + BWI76_RS20595 0.78 +0.6 4,310,187 - BWI76_RS20595 0.79 -1.1 4,310,187 - BWI76_RS20595 0.79 +1.5 4,310,272 - BWI76_RS20595 0.89 +1.1 4,310,394 + -0.4 4,310,414 + +1.3 4,310,660 + BWI76_RS20600 0.15 +0.3 4,310,775 - BWI76_RS20600 0.25 +0.1 4,310,796 + BWI76_RS20600 0.26 +0.3 4,310,804 - BWI76_RS20600 0.27 +0.4 4,310,811 - BWI76_RS20600 0.27 -0.3 4,310,842 + BWI76_RS20600 0.30 +0.8 4,310,849 + BWI76_RS20600 0.30 -1.1 4,311,241 - BWI76_RS20600 0.62 -0.5 4,311,241 - BWI76_RS20600 0.62 +0.9 4,311,255 + BWI76_RS20600 0.63 +0.6 4,311,263 - BWI76_RS20600 0.63 +0.7
Or see this region's nucleotide sequence