Experiment: LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS18065 and BWI76_RS18070 are separated by 78 nucleotides BWI76_RS18070 and BWI76_RS18075 are separated by 6 nucleotides BWI76_RS18075 and BWI76_RS18080 are separated by 133 nucleotides BWI76_RS18080 and BWI76_RS18085 are separated by 144 nucleotides
BWI76_RS18065: BWI76_RS18065 - DNA polymerase III subunit theta, at 3,753,460 to 3,753,690
_RS18065
BWI76_RS18070: BWI76_RS18070 - DNA polymerase III subunit epsilon, at 3,753,769 to 3,754,428
_RS18070
BWI76_RS18075: BWI76_RS18075 - oligopeptidase B, at 3,754,435 to 3,756,495
_RS18075
BWI76_RS18080: BWI76_RS18080 - protein YebE, at 3,756,629 to 3,757,309
_RS18080
BWI76_RS18085: BWI76_RS18085 - hypothetical protein, at 3,757,454 to 3,757,816
_RS18085
Position (kb) 3754
3755
3756
3757 Strain fitness (log2 ratio) -1
0
1
2
3
4
5 at 3753.536 kb on + strand, within BWI76_RS18065 at 3753.590 kb on + strand at 3754.286 kb on + strand, within BWI76_RS18070 at 3754.287 kb on - strand, within BWI76_RS18070 at 3754.293 kb on - strand, within BWI76_RS18070 at 3754.385 kb on + strand at 3754.385 kb on + strand at 3754.447 kb on - strand at 3754.661 kb on - strand, within BWI76_RS18075 at 3754.942 kb on - strand, within BWI76_RS18075 at 3755.332 kb on - strand, within BWI76_RS18075 at 3755.332 kb on - strand, within BWI76_RS18075 at 3755.529 kb on + strand, within BWI76_RS18075 at 3755.856 kb on + strand, within BWI76_RS18075 at 3756.276 kb on - strand, within BWI76_RS18075 at 3756.525 kb on - strand at 3756.544 kb on + strand at 3756.562 kb on - strand at 3756.570 kb on - strand at 3756.626 kb on + strand at 3756.824 kb on + strand, within BWI76_RS18080 at 3757.080 kb on + strand, within BWI76_RS18080
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL remove 3,753,536 + BWI76_RS18065 0.33 +0.1 3,753,590 + +1.2 3,754,286 + BWI76_RS18070 0.78 -1.1 3,754,287 - BWI76_RS18070 0.78 -0.4 3,754,293 - BWI76_RS18070 0.79 +0.1 3,754,385 + +0.3 3,754,385 + +2.0 3,754,447 - -0.2 3,754,661 - BWI76_RS18075 0.11 -0.4 3,754,942 - BWI76_RS18075 0.25 +0.6 3,755,332 - BWI76_RS18075 0.44 +0.2 3,755,332 - BWI76_RS18075 0.44 +5.4 3,755,529 + BWI76_RS18075 0.53 +1.1 3,755,856 + BWI76_RS18075 0.69 +0.9 3,756,276 - BWI76_RS18075 0.89 -0.9 3,756,525 - +0.7 3,756,544 + +0.7 3,756,562 - +0.6 3,756,570 - +0.4 3,756,626 + -0.0 3,756,824 + BWI76_RS18080 0.29 +0.1 3,757,080 + BWI76_RS18080 0.66 +0.2
Or see this region's nucleotide sequence