Experiment: LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16365 and BWI76_RS16370 are separated by 0 nucleotides BWI76_RS16370 and BWI76_RS16375 are separated by 10 nucleotides BWI76_RS16375 and BWI76_RS16380 are separated by 3 nucleotides
BWI76_RS16365: BWI76_RS16365 - electron transport complex subunit RsxC, at 3,394,754 to 3,396,814
_RS16365
BWI76_RS16370: BWI76_RS16370 - electron transport complex subunit D, at 3,396,815 to 3,397,867
_RS16370
BWI76_RS16375: BWI76_RS16375 - electron transport complex subunit G, at 3,397,878 to 3,398,498
_RS16375
BWI76_RS16380: BWI76_RS16380 - electron transport complex subunit RsxE, at 3,398,502 to 3,399,200
_RS16380
Position (kb) 3396
3397
3398 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 3396.157 kb on - strand, within BWI76_RS16365 at 3396.239 kb on + strand, within BWI76_RS16365 at 3396.242 kb on - strand, within BWI76_RS16365 at 3396.464 kb on + strand, within BWI76_RS16365 at 3396.730 kb on - strand at 3396.996 kb on + strand, within BWI76_RS16370 at 3396.996 kb on + strand, within BWI76_RS16370 at 3397.308 kb on + strand, within BWI76_RS16370 at 3397.364 kb on + strand, within BWI76_RS16370 at 3397.622 kb on + strand, within BWI76_RS16370 at 3397.991 kb on - strand, within BWI76_RS16375 at 3398.080 kb on + strand, within BWI76_RS16375 at 3398.406 kb on - strand, within BWI76_RS16375 at 3398.447 kb on - strand at 3398.628 kb on - strand, within BWI76_RS16380 at 3398.759 kb on + strand, within BWI76_RS16380
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL remove 3,396,157 - BWI76_RS16365 0.68 -0.9 3,396,239 + BWI76_RS16365 0.72 -0.3 3,396,242 - BWI76_RS16365 0.72 -2.4 3,396,464 + BWI76_RS16365 0.83 -0.4 3,396,730 - -0.3 3,396,996 + BWI76_RS16370 0.17 -2.0 3,396,996 + BWI76_RS16370 0.17 +0.2 3,397,308 + BWI76_RS16370 0.47 -0.6 3,397,364 + BWI76_RS16370 0.52 -0.5 3,397,622 + BWI76_RS16370 0.77 -1.8 3,397,991 - BWI76_RS16375 0.18 +0.5 3,398,080 + BWI76_RS16375 0.33 -1.1 3,398,406 - BWI76_RS16375 0.85 -2.9 3,398,447 - +0.2 3,398,628 - BWI76_RS16380 0.18 +2.8 3,398,759 + BWI76_RS16380 0.37 -1.4
Or see this region's nucleotide sequence