Experiment: LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14340 and BWI76_RS14345 are separated by 285 nucleotides BWI76_RS14345 and BWI76_RS14350 are separated by 235 nucleotides BWI76_RS14350 and BWI76_RS14355 are separated by 294 nucleotides
BWI76_RS14340: BWI76_RS14340 - ATP-binding protein, at 2,995,949 to 2,997,091
_RS14340
BWI76_RS14345: BWI76_RS14345 - hypothetical protein, at 2,997,377 to 3,000,175
_RS14345
BWI76_RS14350: BWI76_RS14350 - hypothetical protein, at 3,000,411 to 3,000,611
_RS14350
BWI76_RS14355: BWI76_RS14355 - Low calcium response locus protein T, at 3,000,906 to 3,000,980
_RS14355
Position (kb)
2997
2998
2999
3000
3001 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2996.397 kb on + strand, within BWI76_RS14340 at 2996.397 kb on + strand, within BWI76_RS14340 at 2996.438 kb on - strand, within BWI76_RS14340 at 2996.438 kb on - strand, within BWI76_RS14340 at 2996.472 kb on - strand, within BWI76_RS14340 at 2996.531 kb on + strand at 2996.592 kb on + strand, within BWI76_RS14340 at 2996.609 kb on + strand, within BWI76_RS14340 at 2996.609 kb on + strand, within BWI76_RS14340 at 2996.816 kb on - strand, within BWI76_RS14340 at 2996.819 kb on + strand, within BWI76_RS14340 at 2996.831 kb on + strand, within BWI76_RS14340 at 2996.867 kb on + strand, within BWI76_RS14340 at 2996.882 kb on + strand, within BWI76_RS14340 at 2996.921 kb on + strand, within BWI76_RS14340 at 2997.016 kb on + strand at 2997.024 kb on - strand at 2997.104 kb on + strand at 2997.362 kb on - strand at 2997.749 kb on + strand, within BWI76_RS14345 at 2997.752 kb on - strand, within BWI76_RS14345 at 2997.754 kb on + strand, within BWI76_RS14345 at 2997.772 kb on - strand, within BWI76_RS14345 at 2997.865 kb on + strand, within BWI76_RS14345 at 2997.930 kb on - strand, within BWI76_RS14345 at 2997.992 kb on + strand, within BWI76_RS14345 at 2997.992 kb on + strand, within BWI76_RS14345 at 2998.189 kb on - strand, within BWI76_RS14345 at 2998.354 kb on + strand, within BWI76_RS14345 at 2998.405 kb on - strand, within BWI76_RS14345 at 2998.582 kb on + strand, within BWI76_RS14345 at 2998.730 kb on + strand, within BWI76_RS14345 at 2998.777 kb on - strand, within BWI76_RS14345 at 2998.855 kb on + strand, within BWI76_RS14345 at 2999.124 kb on - strand, within BWI76_RS14345 at 2999.134 kb on - strand, within BWI76_RS14345 at 2999.155 kb on - strand, within BWI76_RS14345 at 2999.247 kb on + strand, within BWI76_RS14345 at 2999.372 kb on + strand, within BWI76_RS14345 at 2999.379 kb on + strand, within BWI76_RS14345 at 2999.380 kb on - strand, within BWI76_RS14345 at 2999.380 kb on - strand, within BWI76_RS14345 at 2999.384 kb on + strand, within BWI76_RS14345 at 2999.412 kb on + strand, within BWI76_RS14345 at 2999.465 kb on + strand, within BWI76_RS14345 at 2999.481 kb on + strand, within BWI76_RS14345 at 2999.994 kb on - strand at 3000.024 kb on - strand at 3000.271 kb on + strand at 3000.350 kb on - strand at 3000.376 kb on + strand at 3000.445 kb on - strand, within BWI76_RS14350 at 3000.494 kb on + strand, within BWI76_RS14350 at 3000.503 kb on + strand, within BWI76_RS14350 at 3000.536 kb on + strand, within BWI76_RS14350 at 3000.544 kb on - strand, within BWI76_RS14350 at 3000.544 kb on - strand, within BWI76_RS14350 at 3000.544 kb on - strand, within BWI76_RS14350 at 3000.544 kb on - strand, within BWI76_RS14350 at 3000.580 kb on - strand, within BWI76_RS14350 at 3000.660 kb on - strand at 3000.722 kb on - strand at 3000.758 kb on + strand at 3000.813 kb on + strand at 3000.813 kb on + strand at 3000.821 kb on - strand at 3000.844 kb on + strand at 3000.844 kb on + strand at 3000.844 kb on + strand at 3000.844 kb on + strand at 3000.852 kb on - strand at 3000.852 kb on - strand at 3000.867 kb on - strand at 3000.881 kb on - strand at 3000.887 kb on + strand at 3000.895 kb on - strand at 3000.926 kb on + strand, within BWI76_RS14355 at 3000.953 kb on + strand, within BWI76_RS14355 at 3001.015 kb on - strand at 3001.048 kb on + strand at 3001.070 kb on + strand at 3001.093 kb on + strand at 3001.095 kb on + strand at 3001.095 kb on + strand at 3001.096 kb on + strand at 3001.104 kb on - strand at 3001.106 kb on + strand at 3001.106 kb on + strand at 3001.106 kb on + strand at 3001.106 kb on + strand at 3001.114 kb on - strand at 3001.114 kb on - strand at 3001.123 kb on + strand at 3001.129 kb on + strand at 3001.135 kb on + strand at 3001.142 kb on + strand at 3001.142 kb on + strand at 3001.145 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL remove 2,996,397 + BWI76_RS14340 0.39 -2.7 2,996,397 + BWI76_RS14340 0.39 -0.9 2,996,438 - BWI76_RS14340 0.43 -0.5 2,996,438 - BWI76_RS14340 0.43 -1.6 2,996,472 - BWI76_RS14340 0.46 -2.0 2,996,531 + -0.1 2,996,592 + BWI76_RS14340 0.56 -0.9 2,996,609 + BWI76_RS14340 0.58 -1.1 2,996,609 + BWI76_RS14340 0.58 +0.2 2,996,816 - BWI76_RS14340 0.76 +0.8 2,996,819 + BWI76_RS14340 0.76 +0.9 2,996,831 + BWI76_RS14340 0.77 -0.0 2,996,867 + BWI76_RS14340 0.80 +0.7 2,996,882 + BWI76_RS14340 0.82 -1.8 2,996,921 + BWI76_RS14340 0.85 +0.3 2,997,016 + -1.1 2,997,024 - -0.8 2,997,104 + +0.3 2,997,362 - +0.3 2,997,749 + BWI76_RS14345 0.13 -0.9 2,997,752 - BWI76_RS14345 0.13 -1.1 2,997,754 + BWI76_RS14345 0.13 -1.1 2,997,772 - BWI76_RS14345 0.14 -0.7 2,997,865 + BWI76_RS14345 0.17 -1.1 2,997,930 - BWI76_RS14345 0.20 -0.6 2,997,992 + BWI76_RS14345 0.22 +0.1 2,997,992 + BWI76_RS14345 0.22 -1.9 2,998,189 - BWI76_RS14345 0.29 +0.1 2,998,354 + BWI76_RS14345 0.35 -0.6 2,998,405 - BWI76_RS14345 0.37 +0.2 2,998,582 + BWI76_RS14345 0.43 +0.8 2,998,730 + BWI76_RS14345 0.48 -0.8 2,998,777 - BWI76_RS14345 0.50 -2.6 2,998,855 + BWI76_RS14345 0.53 -1.6 2,999,124 - BWI76_RS14345 0.62 -1.5 2,999,134 - BWI76_RS14345 0.63 -0.0 2,999,155 - BWI76_RS14345 0.64 -1.4 2,999,247 + BWI76_RS14345 0.67 +0.5 2,999,372 + BWI76_RS14345 0.71 -2.5 2,999,379 + BWI76_RS14345 0.72 -1.3 2,999,380 - BWI76_RS14345 0.72 +0.5 2,999,380 - BWI76_RS14345 0.72 -0.2 2,999,384 + BWI76_RS14345 0.72 +1.2 2,999,412 + BWI76_RS14345 0.73 -3.8 2,999,465 + BWI76_RS14345 0.75 -0.0 2,999,481 + BWI76_RS14345 0.75 -0.6 2,999,994 - -1.2 3,000,024 - -1.0 3,000,271 + -1.8 3,000,350 - -0.1 3,000,376 + -2.0 3,000,445 - BWI76_RS14350 0.17 -0.6 3,000,494 + BWI76_RS14350 0.41 -0.1 3,000,503 + BWI76_RS14350 0.46 -1.6 3,000,536 + BWI76_RS14350 0.62 +0.4 3,000,544 - BWI76_RS14350 0.66 +0.5 3,000,544 - BWI76_RS14350 0.66 +0.9 3,000,544 - BWI76_RS14350 0.66 +0.2 3,000,544 - BWI76_RS14350 0.66 +0.8 3,000,580 - BWI76_RS14350 0.84 -0.6 3,000,660 - -0.4 3,000,722 - +1.2 3,000,758 + -1.9 3,000,813 + -0.8 3,000,813 + +1.2 3,000,821 - -0.7 3,000,844 + +0.1 3,000,844 + +0.8 3,000,844 + +0.2 3,000,844 + -2.7 3,000,852 - -1.4 3,000,852 - -0.0 3,000,867 - -0.3 3,000,881 - -2.1 3,000,887 + -0.8 3,000,895 - -2.0 3,000,926 + BWI76_RS14355 0.27 +0.8 3,000,953 + BWI76_RS14355 0.63 +2.8 3,001,015 - -0.4 3,001,048 + -0.0 3,001,070 + -0.9 3,001,093 + +0.2 3,001,095 + +0.5 3,001,095 + +0.8 3,001,096 + +0.7 3,001,104 - -0.4 3,001,106 + +0.2 3,001,106 + -1.8 3,001,106 + +1.2 3,001,106 + -0.6 3,001,114 - -2.0 3,001,114 - -0.7 3,001,123 + -0.4 3,001,129 + +2.5 3,001,135 + -0.7 3,001,142 + -1.1 3,001,142 + -0.6 3,001,145 - -1.4
Or see this region's nucleotide sequence