Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS09540

Experiment: LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS09530 and BWI76_RS09535 overlap by 4 nucleotidesBWI76_RS09535 and BWI76_RS09540 overlap by 4 nucleotidesBWI76_RS09540 and BWI76_RS09545 are separated by 96 nucleotides BWI76_RS09530: BWI76_RS09530 - hypothetical protein, at 1,993,567 to 1,993,890 _RS09530 BWI76_RS09535: BWI76_RS09535 - N-acetylmuramoyl-L-alanine amidase amid, at 1,993,887 to 1,994,717 _RS09535 BWI76_RS09540: BWI76_RS09540 - hypothetical protein, at 1,994,714 to 1,995,727 _RS09540 BWI76_RS09545: BWI76_RS09545 - NAD-dependent epimerase/dehydratase, at 1,995,824 to 1,997,257 _RS09545 Position (kb) 1994 1995 1996Strain fitness (log2 ratio) -1 0 1 2at 1993.846 kb on + strand, within BWI76_RS09530at 1993.990 kb on + strand, within BWI76_RS09535at 1994.103 kb on - strand, within BWI76_RS09535at 1994.244 kb on - strand, within BWI76_RS09535at 1994.512 kb on + strand, within BWI76_RS09535at 1994.582 kb on + strand, within BWI76_RS09535at 1994.736 kb on + strandat 1994.934 kb on + strand, within BWI76_RS09540at 1995.367 kb on + strand, within BWI76_RS09540at 1995.592 kb on - strand, within BWI76_RS09540at 1995.999 kb on - strand, within BWI76_RS09545at 1995.999 kb on - strand, within BWI76_RS09545at 1996.322 kb on - strand, within BWI76_RS09545at 1996.669 kb on - strand, within BWI76_RS09545

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Per-strain Table

Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL
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1,993,846 + BWI76_RS09530 0.86 +0.4
1,993,990 + BWI76_RS09535 0.12 -0.8
1,994,103 - BWI76_RS09535 0.26 +0.2
1,994,244 - BWI76_RS09535 0.43 +1.2
1,994,512 + BWI76_RS09535 0.75 -1.2
1,994,582 + BWI76_RS09535 0.84 +0.4
1,994,736 + +0.6
1,994,934 + BWI76_RS09540 0.22 +2.2
1,995,367 + BWI76_RS09540 0.64 +0.6
1,995,592 - BWI76_RS09540 0.87 +0.7
1,995,999 - BWI76_RS09545 0.12 +0.1
1,995,999 - BWI76_RS09545 0.12 +0.2
1,996,322 - BWI76_RS09545 0.35 -0.4
1,996,669 - BWI76_RS09545 0.59 -0.1

Or see this region's nucleotide sequence