Experiment: LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS09100 and BWI76_RS09105 are separated by 52 nucleotides BWI76_RS09105 and BWI76_RS09110 are separated by 1 nucleotides BWI76_RS09110 and BWI76_RS09115 are separated by 182 nucleotides
BWI76_RS09100: BWI76_RS09100 - glutathione ABC transporter substrate-binding protein GsiB, at 1,922,108 to 1,923,646
_RS09100
BWI76_RS09105: BWI76_RS09105 - glutathione ABC transporter permease GsiC, at 1,923,699 to 1,924,619
_RS09105
BWI76_RS09110: BWI76_RS09110 - glutathione ABC transporter permease GsiD, at 1,924,621 to 1,925,532
_RS09110
BWI76_RS09115: BWI76_RS09115 - 30S ribosomal protein S12 methylthiotransferase RimO, at 1,925,715 to 1,927,040
_RS09115
Position (kb) 1924
1925
1926 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 1923.992 kb on + strand, within BWI76_RS09105 at 1924.096 kb on - strand, within BWI76_RS09105 at 1924.452 kb on + strand, within BWI76_RS09105 at 1924.671 kb on + strand at 1924.681 kb on + strand at 1924.754 kb on + strand, within BWI76_RS09110 at 1924.779 kb on + strand, within BWI76_RS09110 at 1924.996 kb on + strand, within BWI76_RS09110 at 1925.639 kb on + strand at 1925.647 kb on - strand at 1925.647 kb on - strand at 1925.654 kb on + strand at 1926.086 kb on - strand, within BWI76_RS09115 at 1926.161 kb on + strand at 1926.194 kb on + strand, within BWI76_RS09115 at 1926.377 kb on + strand, within BWI76_RS09115
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL remove 1,923,992 + BWI76_RS09105 0.32 -0.2 1,924,096 - BWI76_RS09105 0.43 -0.0 1,924,452 + BWI76_RS09105 0.82 +0.3 1,924,671 + -0.6 1,924,681 + -1.1 1,924,754 + BWI76_RS09110 0.15 -1.1 1,924,779 + BWI76_RS09110 0.17 -1.3 1,924,996 + BWI76_RS09110 0.41 -3.7 1,925,639 + +0.3 1,925,647 - +0.4 1,925,647 - -1.1 1,925,654 + -1.2 1,926,086 - BWI76_RS09115 0.28 -0.2 1,926,161 + +1.2 1,926,194 + BWI76_RS09115 0.36 -0.0 1,926,377 + BWI76_RS09115 0.50 +0.2
Or see this region's nucleotide sequence